Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 May;77(5):2656–2660. doi: 10.1073/pnas.77.5.2656

Physical mapping of primary and secondary origins of bacteriophage T7 DNA replication.

F Tamanoi, H Saito, C C Richardson
PMCID: PMC349461  PMID: 6930658

Abstract

Deletion mutants of bacteriophage T7 have been used to identify and to map, by electron microscopy, the origins of T7 DNA replication. The primary origin of phage T7 DNA replication lies within a 100-base-pair region located 14.75-15.0% of the distance from the genetic left end of the DNA molecule. T7 phage whose DNA contains a deletion of this region initiate replication at secondary origins, the predominant one of which is located at a distance approximately 4% from the left end of the molecule.

Full text

PDF
2656

Images in this article

2657Image
on p.2657
2659Image
on p.2659

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burck K. B., Scraba D. G., Miller R. C., Jr Electron microscopic analysis of partially replicated bacteriophage T7 DNA. J Virol. 1979 Nov;32(2):606–613. doi: 10.1128/jvi.32.2.606-613.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Campbell J. L., Richardson C. C., Studier F. W. Genetic recombination and complementation between bacteriophage T7 and cloned fragments of T7 DNA. Proc Natl Acad Sci U S A. 1978 May;75(5):2276–2280. doi: 10.1073/pnas.75.5.2276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Campbell J. L., Tamanoi F., Richardson C. C., Studier F. W. Cloning of the T7 genome in Escherichia coli: use of recombination between cloned sequences and bacteriophage T7 to identify genes involved in recombination and a clone containing the origin of T7 DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):441–448. doi: 10.1101/sqb.1979.043.01.050. [DOI] [PubMed] [Google Scholar]
  4. Dressler D., Wolfson J., Magazin M. Initiation and reinitiation of DNA synthesis during replication of bacteriophage T7. Proc Natl Acad Sci U S A. 1972 Apr;69(4):998–1002. doi: 10.1073/pnas.69.4.998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Halpern M. E., Mattson T., Kozinski A. W. Origins of phage T4 DNA replication as revealed by hybridization to cloned genes. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6137–6141. doi: 10.1073/pnas.76.12.6137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hausmann R. Bacteriophage T7 genetics. Curr Top Microbiol Immunol. 1976;75:77–110. doi: 10.1007/978-3-642-66530-1_3. [DOI] [PubMed] [Google Scholar]
  7. Hillenbrand G., Morelli G., Lanka E., Scherzinger E. Bacteriophage T7 DNA primase: a multifunctional enzyme involved in DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):449–459. doi: 10.1101/sqb.1979.043.01.051. [DOI] [PubMed] [Google Scholar]
  8. Howe C. C., Buckley P. J., Carlson K. M., Kozinski A. W. Multiple and specific initiation of T4 DNA replication. J Virol. 1973 Jul;12(1):130–148. doi: 10.1128/jvi.12.1.130-148.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kolodner R., Tewari K. K. Presence of displacement loops in the covalently closed circular chloroplast deoxyribonucleic acid from higher plants. J Biol Chem. 1975 Nov 25;250(22):8840–8847. [PubMed] [Google Scholar]
  10. LeClerc J. E., Richardson C. C. Gene 2 protein of bacteriophage T7: purification and requirement for packaging of T7 DNA in vitro. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4852–4856. doi: 10.1073/pnas.76.10.4852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moore D. D., Denniston-Thompson K., Kruger K. E., Furth M. E., Williams B. G., Daniels D. L., Blattner F. R. Dissection and comparative anatomy of the origins of replication of lambdoid phages. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):155–163. doi: 10.1101/sqb.1979.043.01.022. [DOI] [PubMed] [Google Scholar]
  12. Oakley J. L., Coleman J. E. Structure of a promoter for T7 RNA polymerase. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4266–4270. doi: 10.1073/pnas.74.10.4266. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Panayotatos N., Wells R. D. Cloning and localization of the in vitro functional origin of replication of bacteriophage T7 DNA. J Biol Chem. 1979 Jun 25;254(12):5555–5561. [PubMed] [Google Scholar]
  14. Panayotatos N., Wells R. D. Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence. Nature. 1979 Jul 5;280(5717):35–39. doi: 10.1038/280035a0. [DOI] [PubMed] [Google Scholar]
  15. Richardson C. C., Romano L. J., Kolodner R., LeClerc J. E., Tamanoi F., Engler M. J., Dean F. B., Richardson D. S. Replication of bacteriophage T7 DNA by purified proteins. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):427–440. doi: 10.1101/sqb.1979.043.01.049. [DOI] [PubMed] [Google Scholar]
  16. Robertson H. D., Dickson E., Dunn J. J. A nucleotide sequence from a ribonuclease III processing site in bacteriophage T7 RNA. Proc Natl Acad Sci U S A. 1977 Mar;74(3):822–826. doi: 10.1073/pnas.74.3.822. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Romano L. J., Richardson C. C. Characterization of the ribonucleic acid primers and the deoxyribonucleic acid product synthesized by the DNA polymerase and gene 4 protein of bacteriophage T7. J Biol Chem. 1979 Oct 25;254(20):10483–10489. [PubMed] [Google Scholar]
  18. Romano L. J., Richardson C. C. Requirements for synthesis of ribonucleic acid primers during lagging strand synthesis by the DNA polymerase and gene 4 protein of bacteriophage T7. J Biol Chem. 1979 Oct 25;254(20):10476–10482. [PubMed] [Google Scholar]
  19. Simon M. N., Studier F. W. Physical mapping of the early region of bacteriophage T7 DNA. J Mol Biol. 1973 Sep 15;79(2):249–265. doi: 10.1016/0022-2836(73)90004-1. [DOI] [PubMed] [Google Scholar]
  20. Studier F. W., Rosenberg A. H., Simon M. N., Dunn J. J. Genetic and physical mapping in the early region of bacteriophage T7 DNA. J Mol Biol. 1979 Dec 25;135(4):917–937. doi: 10.1016/0022-2836(79)90520-5. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. The genetics and physiology of bacteriophage T7. Virology. 1969 Nov;39(3):562–574. doi: 10.1016/0042-6822(69)90104-4. [DOI] [PubMed] [Google Scholar]
  22. Tomizawa J., Selzer G. Initiation of DNA synthesis in Escherichia coli. Annu Rev Biochem. 1979;48:999–1034. doi: 10.1146/annurev.bi.48.070179.005031. [DOI] [PubMed] [Google Scholar]
  23. Wolfson J., Dressler D., Magazin M. Bacteriophage T7 DNA replication: a linear replicating intermediate (gradient centrifugation-electron microscopy-E. coli-DNA partial denaturation). Proc Natl Acad Sci U S A. 1972 Feb;69(2):499–504. doi: 10.1073/pnas.69.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES