Abstract
The interaction of the synthetic serum thymic factor (FTS, "facteur thymique sérique") with human T lymphocytes from two established T cell lines (1301 and HSB2) was studied by using [3H]FTS. At 30 degrees C, in the presence of 10 nM labeled FTS, a steady state was observed within 120 min. Bound labeled FTS was dissociated by simultaneous addition of an excess of unlabeled FTS (dissociation constant Kd = 3 nM). The binding of [3H]FTS was reduced to 10-20% when unlabeled FTS was added at 20 microgram/ml (20 micro M), but not when unrelated peptides or FTS analogues differing from FTS by only one amino acid residue were added. No FTS binding was detectable on B and null cell lines or on two T cell lines other than 1301 and HSB2. These data indicate that only certain T cell subsets possess receptors with high affinity and specificity for FTS.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bach J. F., Dardenne M., Goldstein A. L., Guha A., White A. Appearance of T-cell markers in bone marrow rosette-forming cells after incubation with thymosin, a thymic hormone. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2734–2738. doi: 10.1073/pnas.68.11.2734. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bach J. F., Dardenne M. Studies on thymus products. II. Demonstration and characterization of a circulating thymic hormone. Immunology. 1973 Sep;25(3):353–366. [PMC free article] [PubMed] [Google Scholar]
- Dardenne M., Pleau J. M., Man N. K., Bach J. F. Structural study of circulating thymic factor: a peptide isolated from pig serum. I. Isolation and purification. J Biol Chem. 1977 Nov 25;252(22):8040–8044. [PubMed] [Google Scholar]
- De la Llosa P., Marche P., Morgat J. L., De la Llosa-Hermier M. P. A new procedure for labelling luteinizing hormone with tritium. FEBS Lett. 1974 Sep 1;45(1):162–165. doi: 10.1016/0014-5793(74)80836-7. [DOI] [PubMed] [Google Scholar]
- Lesniak M. A., Gorden P., Roth J., Gavin J. R., 3rd Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J Biol Chem. 1974 Mar 25;249(6):1661–1667. [PubMed] [Google Scholar]
- Pleau J. M., Dardenne M., Blouquit Y., Bach J. F. Structural study of circulating thymic factor: a peptide isolated from pig serum. II. Amino acid sequence. J Biol Chem. 1977 Nov 25;252(22):8045–8047. [PubMed] [Google Scholar]
- Preud'homme J. L., Gonnot M., Fellous M., Seligmann M. Receptors for IgM on human lymphoblastoid B and T cell lines. Scand J Immunol. 1979;10(3):207–211. doi: 10.1111/j.1365-3083.1979.tb01341.x. [DOI] [PubMed] [Google Scholar]
- Roth J. Peptide hormone binding to receptors: a review of direct studies in vitro. Metabolism. 1973 Aug;22(8):1059–1073. doi: 10.1016/0026-0495(73)90225-4. [DOI] [PubMed] [Google Scholar]