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. 1980 May;77(5):2951–2954. doi: 10.1073/pnas.77.5.2951

Multiple components of blood group A and B antigens in human erythrocyte membranes and their difference between A1 and A2 status.

H Fujii, A Yoshida
PMCID: PMC349524  PMID: 6156460

Abstract

Human type O erythrocyte membranes were converted to type A1 by purified human A1-enzyme, to type A2 by purified human A2-enzyme, and to type B by purified human B-enzyme in the presence of radioactive sugar donors (i.e., UDP-N-acetylgalactosamine for A-enzyme and UDP-galactose for B-enzyme, respectively). Type A2 erythrocyte membranes were also converted to type A1 by purified A1-enzyme A1-enzyme. The labeled blood group antigens (A1, A2, and B) thus produced were analyzed by sodium dodecyl sulfate gel electrophoresis and by isoelectric focusing. It was demonstrated that blood group antigens on erythrocyte membranes are mostly (greater than 80%) glycolipid, not glycoprotein. The blood group glycolipids were presumably conjugated with other membrane materials and formed three major components in type A1 and type B erythrocyte membranes. However, two of the three components were absent in type O erythrocyte membranes converted to type A2. These results indicate the heterogeneity of blood group components in erythrocytes membranes and the qualitative difference between A1 and A2 antigens on these membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIRD G. W. G. Relationship of the blood sub-groups A1, A2 and A1B, A2B to haemagglutinins present in the seeds of Dolichos biflorus. Nature. 1952 Oct 18;170(4329):674–674. doi: 10.1038/170674a0. [DOI] [PubMed] [Google Scholar]
  2. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  3. Dejter-Juszynski M., Harpaz N., Flowers H. M., Sharon N. Blood-group ABH-specific macroglycolipids of human erythrocytes: isolation in high yield from a crude membrane glycoprotein fraction. Eur J Biochem. 1978 Feb;83(2):363–373. doi: 10.1111/j.1432-1033.1978.tb12102.x. [DOI] [PubMed] [Google Scholar]
  4. Economidou J., Hughes-Jones N. C., Gardner B. Quantitative measurements concerning A and B antigen sites. Vox Sang. 1967 May;12(5):321–328. doi: 10.1111/j.1423-0410.1967.tb03362.x. [DOI] [PubMed] [Google Scholar]
  5. Fujita S., Cleve H. Isolation and partial characterization of two minor glycoproteins from human erythrocyte membranes. Biochim Biophys Acta. 1975 Mar 13;382(2):172–180. doi: 10.1016/0005-2736(75)90175-3. [DOI] [PubMed] [Google Scholar]
  6. Gardas A. Structure of an (A-blood-group)-active glycolipid isolated from human erythrocytes. Eur J Biochem. 1978 Sep 1;89(2):471–473. doi: 10.1111/j.1432-1033.1978.tb12550.x. [DOI] [PubMed] [Google Scholar]
  7. Hakomori S., Stellner K., Watanabe K. Four antigenic variants of blood group A glycolipid: examples of highly complex, branched chain glycolipid of animal cell membrane. Biochem Biophys Res Commun. 1972 Nov 15;49(4):1061–1068. doi: 10.1016/0006-291x(72)90320-8. [DOI] [PubMed] [Google Scholar]
  8. Hakomori S., Strycharz G. D. Investigations on cellular blood-group substances. I. Isolation and chemical composition of blood-group ABH and Le-b isoantigens of sphingoglycolipid nature. Biochemistry. 1968 Apr;7(4):1279–1286. doi: 10.1021/bi00844a005. [DOI] [PubMed] [Google Scholar]
  9. Hamaguchi H., Cleve H. Solubilization of human erythrocyte membrane glycoproteins and separation of the MN glycoprotein from a glycoprotein with I, S, and A activity. Biochim Biophys Acta. 1972 Sep 29;278(2):271–280. doi: 10.1016/0005-2795(72)90232-2. [DOI] [PubMed] [Google Scholar]
  10. Hearn V. M., Smith Z. G., Watkins W. M. An a-N-acetyl-D-galactosaminyltransferase associated with the human blood-group A character. Biochem J. 1968 Sep;109(2):315–317. doi: 10.1042/bj1090315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kościelak J., Miller-Podraza H., Krauze R., Piasek A. Isolation and characterization of poly(glycosyl)ceramides (megaloglycolipids) with A, H and I blood-group activities. Eur J Biochem. 1976 Dec;71(1):9–18. doi: 10.1111/j.1432-1033.1976.tb11083.x. [DOI] [PubMed] [Google Scholar]
  12. Kościelak J., Plasek A., Górniak H., Gardas A., Gregor A. Structures of fucose-containing glycolipids with H and B blood-group activity and of sialic acid and glucosamine-containing glycolipid of human-erythrocyte membrane. Eur J Biochem. 1973 Aug 17;37(2):214–225. doi: 10.1111/j.1432-1033.1973.tb02978.x. [DOI] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Marchesi V. T., Andrews E. P. Glycoproteins: isolation from cellmembranes with lithium diiodosalicylate. Science. 1971 Dec 17;174(4015):1247–1248. doi: 10.1126/science.174.4015.1247. [DOI] [PubMed] [Google Scholar]
  15. Moreno C., Lundblad A., Kabat B. A. Immunochemical studies on blood groups. LI. A comparative study of the reaction of A 1 and A 2 blood group glycoproteins with human anti-A. J Exp Med. 1971 Aug 1;134(2):439–457. doi: 10.1084/jem.134.2.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nagai M., Davè V., Kaplan B. E., Yoshida A. Human blood group glycosyltransferases. I. Purification of n-acetylgalactosaminyltransferase. J Biol Chem. 1978 Jan 25;253(2):377–379. [PubMed] [Google Scholar]
  17. Nagai M., Davè V., Muensch H., Yoshida A. Human blood group glycosyltransferase. II. Purification of galactosyltransferase. J Biol Chem. 1978 Jan 25;253(2):380–381. [PubMed] [Google Scholar]
  18. O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
  19. Poulik M. D., Lauf P. K. Heterogeneity of water-soluble structural components of human red cell membrane. Nature. 1965 Nov 27;208(5013):874–876. doi: 10.1038/208874a0. [DOI] [PubMed] [Google Scholar]
  20. Prat J. P., Lamy J. N., Weill J. D. Coloration des lipoprotéines après électrophorèse en gel de polyacrylamide. Bull Soc Chim Biol (Paris) 1969 Dec 18;51(9):1367–1367. [PubMed] [Google Scholar]
  21. Race C., Ziderman D., Watkins W. M. An alpha-d-galactosyltransferase associated with the blood-group B character. Biochem J. 1968 May;107(5):733–735. doi: 10.1042/bj1070733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schachter H., Michaels M. A., Tilley C. A., Crookston M. C., Crookston J. H. Qualitative differences in the N-acetyl-D-galactosaminyltransferases produced by human A1 and A2 genes. Proc Natl Acad Sci U S A. 1973 Jan;70(1):220–224. doi: 10.1073/pnas.70.1.220. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schenkel-Brunner H., Tuppy H. Enzymatic conversion of human O into A erythrocytes and of B into AB erythrocytes. Nature. 1969 Sep 20;223(5212):1272–1273. doi: 10.1038/2231272a0. [DOI] [PubMed] [Google Scholar]
  24. Schwyzer M., Hill R. L. Porcine A blood group-specific N-acetylgalactosaminyltransferase. J Biol Chem. 1977 Apr 10;252(7):2346–2355. [PubMed] [Google Scholar]
  25. Stellner K., Watanabe K., Hakomori S. Isolation and characterization of glycosphingolipids with blood group H specificity from membranes of human erythrocytes. Biochemistry. 1973 Feb;12(4):656–661. doi: 10.1021/bi00728a014. [DOI] [PubMed] [Google Scholar]
  26. Takasaki S., Kobata A. Chemical characterization and distribution of ABO blood group active glycoprotein in human erythrocyte membrane. J Biol Chem. 1976 Jun 25;251(12):3610–3615. [PubMed] [Google Scholar]
  27. Takasaki S., Yamashita K., Kobata A. The sugar chain structures of ABO blood group active glycoproteins obtained from human erythrocyte membrane. J Biol Chem. 1978 Sep 10;253(17):6086–6091. [PubMed] [Google Scholar]
  28. Tishler P. V., Epstein C. J. A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry. Anal Biochem. 1968 Jan;22(1):89–98. doi: 10.1016/0003-2697(68)90262-5. [DOI] [PubMed] [Google Scholar]
  29. Watanabe K., Hakomori S. I. Status of blood group carbohydrate chains in ontogenesis and in oncogenesis. J Exp Med. 1976 Sep 1;144(3):644–653. doi: 10.1084/jem.144.3.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. YAMAKAWA T., IIDA T. Immunochemical study on the red blood cells. I. Globoside, as the agglutinogen of the ABO system on erythrocytes. Jpn J Exp Med. 1953 Aug;23(4):327–331. [PubMed] [Google Scholar]

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