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. 1980 Jun;77(6):3230–3233. doi: 10.1073/pnas.77.6.3230

Characterization of retinoyl beta-glucuronide as a minor metabolite of retinoic acid in bile.

M H Zile, H K Schnoes, H F DeLuca
PMCID: PMC349588  PMID: 6932017

Abstract

Several metabolites detected in the bile of rats given radioactive retinoic acid were separated by liquid/gel partition chromatography and purified by high-pressure liquid chromatography. One of these metabolites was found to be sensitive to beta-D-glucuronidase, yielding both 13-cis- and all-trans-retinoic acid. It had the characteristic ultraviolet absorption spectrum of retinoic acid esters. Trimethylsilyl ether and acetyl derivatives of the methylated metabolite were prepared and examined by mass spectrometry. The resulting mass spectra established the structure to be retinoyl beta-glucuronide. Retinoyl glucuronide was rapidly excreted into the bile: the excretion was complete by 12 hr after the administration of retinoic acid. At this time the metabolite represented 12% of bile radioactivity (10% of dose). These observations confirm the existence of retinoyl glucuronide but demonstrate that it represents only one of several retinoic acid metabolites in bile.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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