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. 1980 Jun;77(6):3322–3326. doi: 10.1073/pnas.77.6.3322

Replication of duplex DNA of phage phi X174 reconstituted with purified enzymes.

K Arai, N Arai, J Shlomai, A Kornberg
PMCID: PMC349607  PMID: 6447874

Abstract

Replication of the covalently closed duplex replicative form (RF) of phage phi X174 DNA has been achieved by coupling two known enzyme systems: (i) synthesis of viral strand circles (SS) from RF, and (ii) conversion of SS to nearly complete RF (RF II). In this coupled system, activated RF (gene A . RF II complex) was a more efficient template and generated as many as 10 RF II molecules per RF input, at a rate commensurate with SS synthesis. The 11 proteins required for the two component systems were all needed in the coupled RF duplication system; no new factors were required. Single-stranded DNA binding protein was needed for RF duplication at only 4% the level needed in its stoichiometric participation in SS synthesis. In addition to RF II, more complex replicative forms appeared late in the reaction, and their possible origin is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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