Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Jun;77(6):3640–3644. doi: 10.1073/pnas.77.6.3640

Subpopulations of human peripheral granulocyes and monocytes express receptors for IgA.

M W Fanger, L Shen, J Pugh, G M Bernier
PMCID: PMC349673  PMID: 6932039

Abstract

Receptors for the Fc portion of immunoglobin on human peripheral blood cells were enumerated by rosette formation with ox erythrocytes sensitized with rabbit IgG, IgA, and IgM. A large percentage of purified polymorphonuclear leukocytes and monocytes were found to express receptors for IgA. These receptors were also found to exist on a significantly greater percentage of lymphocytes than was previously observed. The receptors for IgA were specific, as verified by blocking studies using purified human immunogloblins. In addition, some polymorphonuclear leukocytes and monocytes were observed concomitantly to posses independent receptors for both IgG and IgA. These studies may indicate that IgA can cooperate with monocytes or polymorphonuclear leukocytes through receptors for IgA on these cells and perhaps mediate immune defense on mucosal surfaces. Initial studies on antibody-dependent cellular cytotoxicity suggested that IgA alone is ineffectual in supporting cytolysis by nonactivated human peripheral blood cells.

Full text

PDF
3640

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birch R. E., Bernier G. M., Fanger M. W. Rabbit lymphocytes with receptors for IgM. I. The development of receptors on lymphocytes following intravenous injection of antigen. Cell Immunol. 1979 Sep 1;46(2):348–359. doi: 10.1016/0008-8749(79)90422-2. [DOI] [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. Fanger M. W., Lydyard P. M. Receptors for IgM on human lymphocytes. III. Specificity of receptors. Clin Exp Immunol. 1979 Sep;37(3):495–501. [PMC free article] [PubMed] [Google Scholar]
  4. Greenberg A. H., Shen L., Medley G. Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. I. Phagocytic and non-phagocytic effector cell activity against erythrocyte and tumour target cells in a 51Cr release cytotoxicity assay and [125I]IUdR growth inhibition assay. Immunology. 1975 Oct;29(4):719–729. [PMC free article] [PubMed] [Google Scholar]
  5. Hayward A. R., Layward L., Lydyard P. M., Moretta L., Dagg M., Lawton A. R. Fc-receptor heterogeneity of human suppressor T cells. J Immunol. 1978 Jul;121(1):1–5. [PubMed] [Google Scholar]
  6. Ishizaka T., Ishizaka K., Bennich H., Johansson S. G. Biologic activities of aggregated immunoglobulin E. J Immunol. 1970 Apr;104(4):854–862. [PubMed] [Google Scholar]
  7. Lawrence D. A., Weigle W. O., Spiegelberg H. L. Immunoglobulins cytophilic for human lymphocytes, monocytes, and neutrophils. J Clin Invest. 1975 Feb;55(2):368–376. doi: 10.1172/JCI107940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee S. K., Singh J., Taylor R. B. Subclasses of T cells with different sensitivities to cytotoxic antibody in the presence of anesthetics. Eur J Immunol. 1975 Apr;5(4):259–262. doi: 10.1002/eji.1830050408. [DOI] [PubMed] [Google Scholar]
  9. Lowell G. H., Smith L. F., Artenstein M. S., Nash G. S., MacDermott R. P., Jr Antibody-dependent cell-mediated antibacterial activity of human mononuclear cells. I. K lymphocytes and monocytes are effective against meningococi in cooperation with human imune sera. J Exp Med. 1979 Jul 1;150(1):127–137. doi: 10.1084/jem.150.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lum L. G., Benveniste E., Blaese R. M. Functional properties of human T cells bearing Fc receptors for IgA. I. Mitogen responsiveness, mixed lymphocyte culture reactivity, and helper activity for B cell immunoglobulin production. J Immunol. 1980 Feb;124(2):702–707. [PubMed] [Google Scholar]
  11. Lum L. G., Muchmore A. V., Keren D., Decker J., Koski I., Strober W., Blaese R. M. A receptor for IgA on human T lymphocytes. J Immunol. 1979 Jan;122(1):65–69. [PubMed] [Google Scholar]
  12. Lum L. G., Muchmore A. V., O'Connor N., Strober W., Blaese R. M. Fc receptors for IgA on human B, and human non-B, non-T lymphocytes. J Immunol. 1979 Aug;123(2):714–719. [PubMed] [Google Scholar]
  13. Moretta L., Webb S. R., Grossi C. E., Lydyard P. M., Cooper M. D. Functional analysis of two human T-cell subpopulations: help and suppression of B-cell responses by T cells bearing receptors for IgM or IgG. J Exp Med. 1977 Jul 1;146(1):184–200. doi: 10.1084/jem.146.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reynolds H. Y., Kazmierowski J. A., Newball H. H. Specificity of opsonic antibodies to enhance phagocytosis of Pseudomonas aeruginosa by human alveolar macrophages. J Clin Invest. 1975 Aug;56(2):376–385. doi: 10.1172/JCI108102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reynolds H. Y., Thompson R. E. Pulmonary host defenses. I. Analysis of protein and lipids in bronchial secretions and antibody responses after vaccination with pseudomonas aeruginosa. J Immunol. 1973 Aug;111(2):358–368. [PubMed] [Google Scholar]
  16. Roberts R. B. The relationship between group A and group C meningococcal polysaccharides and serum opsonins in man. J Exp Med. 1970 Mar 1;131(3):499–513. doi: 10.1084/jem.131.3.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Setcavage T. M., Kim Y. B. Inhibition of the immune response by membrane-bound antibody. J Immunol. 1978 Nov;121(5):1706–1710. [PubMed] [Google Scholar]
  18. Spiegelberg H. L., Lawrence D. A., Henson P. Cytophilic properties of IgA to human neutrophils. Adv Exp Med Biol. 1974;45(0):67–74. doi: 10.1007/978-1-4613-4550-3_8. [DOI] [PubMed] [Google Scholar]
  19. Steigbigel R. T., Lambert L. H., Jr, Remington J. S. Phagocytic and bacterial properties of normal human monocytes. J Clin Invest. 1974 Jan;53(1):131–142. doi: 10.1172/JCI107531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strober W., Hague N. E., Lum L. G., Henkart P. A. IgA-Fc receptors on mouse lymphoid cells. J Immunol. 1978 Dec;121(6):2440–2445. [PubMed] [Google Scholar]
  21. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
  22. Van Epps D. E., Reed K., Williams R. C., Jr Suppression of human PMN bactericidal activity by human IgA paraproteins. Cell Immunol. 1978 Mar 15;36(2):363–376. doi: 10.1016/0008-8749(78)90280-0. [DOI] [PubMed] [Google Scholar]
  23. Van Epps D. E., Williams R. C., Jr Suppression of leukocyte chemotaxis by human IgA myeloma components. J Exp Med. 1976 Nov 2;144(5):1227–1242. doi: 10.1084/jem.144.5.1227. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES