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. 1980 Jun;77(6):3720–3724. doi: 10.1073/pnas.77.6.3720

Inhibition of AKR leukemogenesis by SMX-1, a dualtropic murine leukemia virus.

E Stockert, P V O'Donnell, Y Obata, L J Old
PMCID: PMC349690  PMID: 6251478

Abstract

Intrathymic injection of SMX-1, a dualtropic murine leukemia virus (MuLV) originally derived from Moloney murine leukemia virus stocks, protects AKR mice from developing MuLV-accelerated leukemia and spontaneous leukemia. Thymuses of SMX-1-injected mice show no change in weight, morphology, or thymocyte size, and quantitative expression of Thy-1 and Lyt-2 differentiation antigens is identical to control mice. The amplified thymic expression of MuLV-related antigens that occurs spontaneously in 6-month-old preleukemic AKR mice or that can be induced in young AKR mice by leukemogenic AKR dualtropic MuLV is prevented by SMX-1. It appears unlikely that the protective effect of SMX-1 is explicable in terms of cross-immunogenicity with transforming MuLV or transformed cells. As SMX-1 persists for long periods after intrathymic injection and does not alter levels of thymic ecotropic MuLV, SMX-1 may interfere with the generation, spread, or leukemogenicity of dualtropic MuLV that form de novo in AKR thymus during the late preleukemic phase. SMX-1 provides a way to probe the events leading to cell transformation in AKR mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes R. D., Tuffrey M., Ford C. E. Suppression of lymphoma development in tetraparental AKR mouse chimaeras derived from ovum fusion. Nat New Biol. 1973 Aug 29;244(139):282–284. doi: 10.1038/newbio244282a0. [DOI] [PubMed] [Google Scholar]
  2. Bekesi J. G., Roboz J. P., Zimmerman E., Holland J. F. Treatment of spontaneous leukemia in AKR mice with chemotherapy, immunotherapy, or interferon. Cancer Res. 1976 Feb;36(2 Pt 2):631–639. [PubMed] [Google Scholar]
  3. Boyse E. A., Old L. J., Iritani C. A. The technique of obtaining thymocytes from living mice. Transplantation. 1971 Jul;12(1):93–95. doi: 10.1097/00007890-197107000-00019. [DOI] [PubMed] [Google Scholar]
  4. Cloyd M. W., Hartley J. W., Rowe W. P. Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses. J Exp Med. 1980 Mar 1;151(3):542–552. doi: 10.1084/jem.151.3.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Declève A., Niwa O., Hilgers J., Kaplan H. S. An improved murine leukemia virus immunofluorescence assay. Virology. 1974 Feb;57(2):491–502. doi: 10.1016/0042-6822(74)90188-3. [DOI] [PubMed] [Google Scholar]
  6. Elder J. H., Gautsch J. W., Jensen F. C., Lerner R. A., Hartley J. W., Rowe W. P. Biochemical evidence that MCF murine leukemia viruses are envelope (env) gene recombinants. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4676–4680. doi: 10.1073/pnas.74.10.4676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fish D. C., Gilden R. V., Bare R. M., Trimmer R. M., Huebner R. J. Prevention of spontaneous leukemia in AKR mice by passive immunization and type specificity of this protection. J Natl Cancer Inst. 1979 Apr;62(4):943–945. [PubMed] [Google Scholar]
  8. GROSS L. "Spontaneous" leukemia developing in C3H mice following inoculation in infancy, with AK-leukemic extracts, or AK-embrvos. Proc Soc Exp Biol Med. 1951 Jan;76(1):27–32. [PubMed] [Google Scholar]
  9. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. A new class of murine leukemia virus associated with development of spontaneous lymphomas. Proc Natl Acad Sci U S A. 1977 Feb;74(2):789–792. doi: 10.1073/pnas.74.2.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kawashima K., Ikeda H., Hartley J. W., Stockert E., Rowe W. P., Old L. J. Changes in expression of murine leukemia virus antigens and production of xenotropic virus in the late preleukemic period in AKR mice. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4680–4684. doi: 10.1073/pnas.73.12.4680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kawashima K., Ikeda H., Stockert E., Takahashi T., Old L. J. Age-related changes in cell surface antigens of preleukemic AKR thymocytes. J Exp Med. 1976 Jul 1;144(1):193–208. doi: 10.1084/jem.144.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levy J. A. Xenotropic type C viruses. Curr Top Microbiol Immunol. 1978;79:111–213. doi: 10.1007/978-3-642-66853-1_4. [DOI] [PubMed] [Google Scholar]
  13. Nowinski R. C., Hays E. F. Oncogenicity of AKR endogenous leukemia viruses. J Virol. 1978 Jul;27(1):13–18. doi: 10.1128/jvi.27.1.13-18.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. O'Donnell P. V., Stockert E. Induction of GIX antigen and gross cell surface antigen after infection by ecotropic and xenotropic murine leukemia viruses in vitro. J Virol. 1976 Dec;20(3):545–554. doi: 10.1128/jvi.20.3.545-554.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. OLD L. J., BOYSE E. A., STOCKERT E. TYPING OF MOUSE LEUKAEMIAS BY SEROLOGICAL METHODS. Nature. 1964 Feb 22;201:777–779. doi: 10.1038/201777a0. [DOI] [PubMed] [Google Scholar]
  16. Peebles P. T. An in vitro focus-induction assay for xenotropic murine leukemia virus, feline leukemia virus C, and the feline--primate viruses RD-114/CCC/M-7. Virology. 1975 Sep;67(1):288–291. doi: 10.1016/0042-6822(75)90427-4. [DOI] [PubMed] [Google Scholar]
  17. Pollard M., Truitt R. L. Allogeneic bone marrow chimerism in germfree mice. 1. Prevention of spontaneous leukemia in AKR mice. Proc Soc Exp Biol Med. 1973 Nov;144(2):659–665. doi: 10.3181/00379727-144-37657. [DOI] [PubMed] [Google Scholar]
  18. Rommelaere J., Faller D. V., Hopkins N. Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses. Proc Natl Acad Sci U S A. 1978 Jan;75(1):495–499. doi: 10.1073/pnas.75.1.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rowe W. P. Genetic factors in the natural history of murine leukemia virus infection: G. H. A. Clowes Memorial Lecture. Cancer Res. 1973 Dec;33(12):3061–3068. [PubMed] [Google Scholar]
  20. Rowe W. P. Leukemia virus genomes in the chromosomal DNA of the mouse. Harvey Lect. 1978;71:173–192. [PubMed] [Google Scholar]
  21. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  22. Schwarz H., Fischinger P. J., Ihle J. N., Thiel H. J., Weiland F., Bolognesi D. P., Schäfer W. Properties of mouse leukemia viruses. XVI. Suppression of spontaneous fetal leukemias in AKR mice by treatment with broadly reacting antibody against the viral glycoprotein gp 71. Virology. 1979 Feb;93(1):159–174. doi: 10.1016/0042-6822(79)90284-8. [DOI] [PubMed] [Google Scholar]
  23. Stephenson J. R., Reynolds R. K., Aaronson S. A. Isolation of temperature-sensitive mutants of murine leukemia virus. Virology. 1972 Jun;48(3):749–756. doi: 10.1016/0042-6822(72)90158-4. [DOI] [PubMed] [Google Scholar]
  24. Stockert E., DeLeo A. B., O'Donnell P. V., Obata Y., Old L. J. G(AKSL2): a new cell surface antigen of the mouse related to the dualtropic mink cell focus-inducing class of murine leukemia virus detected by naturally occurring antibody. J Exp Med. 1979 Jan 1;149(1):200–215. doi: 10.1084/jem.149.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stockert E., Old L. J., Boyse E. A. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome. J Exp Med. 1971 Jun 1;133(6):1334–1355. doi: 10.1084/jem.133.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]

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