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. 1980 Jul;77(7):3801–3805. doi: 10.1073/pnas.77.7.3801

Interaction between beta-adrenergic receptors and guanine nucleotide sites in turkey erythrocyte membranes.

G Vauquelin, S Bottari, C Andre, B Jacobsson, A D Strosberg
PMCID: PMC349714  PMID: 6253990

Abstract

beta 1-Adrenergic receptors from turkey erythrocyte membranes have been identified by specific binding of the radiolabeled antagonist (-)-[3H]dihydroalprenolol. These receptors are inactivated by the alkylating agent N-ethylmaleimide when occupied by beta-adrenergic agonists but not when occupied by antagonists or when unoccupied. A time-dependent decrease of the number of receptor sites is observed. Inactivation affects 45-60% of the sites, regardless of the agonist or N-ethylmaleimide concentration. The guanine nucleotides GTP and 5'-guanylyl imidodiphosphate effectively protect the receptors against agonist-mediated inactivation by N-ethylmaleimide. Protection by ATP necessitates a 100-fold higher concentration; 10 mM NaF is ineffective. The guanine nucleotide effect is reversible and occurs via interaction with N-ethylmaleimide-insensitive sites. These observations establish that guanine nucleotide sites interact with and caused structural modification of the agonist-occupied beta-adrenergic receptors in turkey erythrocyte membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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