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. 1980 Jul;77(7):4003–4006. doi: 10.1073/pnas.77.7.4003

Molecular cloning of human interferon cDNA.

T Taniguchi, Y Fujii-Kuriyama, M Muramatsu
PMCID: PMC349756  PMID: 6159625

Abstract

A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979) Proc. Jpn. Acad. 55, Ser. B, 464-469]. This conclusion was confirmed by a hybridization-translation assay, using rabbit globin mRNA and its cDNA-containing plasmid as a control. Plasmid TpIF319 was used as probe and another recombinant plasmid, TpIF319-13, whose cDNA insert consists of about 800 base pairs, was isolated. Nucleotide sequence analysis of the cDNA revealed that the DNA in fact codes for human fibroblast interferon.

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Selected References

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