Abstract
The kinetics of sodium-induced aggregation of sonicated phosphatidylserine vesicles has been studied as a function of sodium concentration and temperature. The concentration threshold for aggregation induced by monovalent sodium has been found to be 550 mM sodium by stopped-flow rapid-mixing techniques. This aggregation is completely reversible to changes in sodium ion concentration and to changes in temperature. The aggregation rate decreases with increasing temperature, indicating that the backward reaction rate increases more rapidly with temperature than does the forward rate.
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- Day E. P., Ho J. T., Kunze R. K., Jr, Sun S. T. Dynamic light scattering study of calcium-induced fusion in phospholipid vesicles. Biochim Biophys Acta. 1977 Nov 1;470(3):503–508. doi: 10.1016/0005-2736(77)90142-0. [DOI] [PubMed] [Google Scholar]
- Lansman J., Haynes D. H. Kinetics of a Ca-2+-triggered membrane aggregation reaction of phospholipid membranes. Biochim Biophys Acta. 1975 Jul 3;394(3):335–347. doi: 10.1016/0005-2736(75)90288-6. [DOI] [PubMed] [Google Scholar]
- Liao M. J., Prestegard J. H. Fusion of phosphatidic acid-phosphatidylcholine mixed lipid vesicles. Biochim Biophys Acta. 1979 Jan 19;550(2):157–173. doi: 10.1016/0005-2736(79)90204-9. [DOI] [PubMed] [Google Scholar]
- Papahadjopoulos D., Miller N. Phospholipid model membranes. I. Structural characteristics of hydrated liquid crystals. Biochim Biophys Acta. 1967 Sep 9;135(4):624–638. doi: 10.1016/0005-2736(67)90094-6. [DOI] [PubMed] [Google Scholar]
- Papahadjopoulos D., Vail W. J., Jacobson K., Poste G. Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles. Biochim Biophys Acta. 1975 Jul 3;394(3):483–491. doi: 10.1016/0005-2736(75)90299-0. [DOI] [PubMed] [Google Scholar]
- Papahadjopoulos D., Vail W. J., Newton C., Nir S., Jacobson K., Poste G., Lazo R. Studies on membrane fusion. III. The role of calcium-induced phase changes. Biochim Biophys Acta. 1977 Mar 17;465(3):579–598. doi: 10.1016/0005-2736(77)90275-9. [DOI] [PubMed] [Google Scholar]
- Poste G., Allison A. C. Membrane fusion. Biochim Biophys Acta. 1973 Dec 28;300(4):421–465. doi: 10.1016/0304-4157(73)90015-4. [DOI] [PubMed] [Google Scholar]
- Sun S. T., Day E. P., Ho J. T. Temperature dependence of calcium-induced fusion of sonicated phosphatidylserine vesicles. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4325–4328. doi: 10.1073/pnas.75.9.4325. [DOI] [PMC free article] [PubMed] [Google Scholar]