Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Jul;77(7):4079–4083. doi: 10.1073/pnas.77.7.4079

Uniparental propagation of mitochondrial DNA in mouse-human cell hybrids.

L De Francesco, G Attardi, C M Croce
PMCID: PMC349773  PMID: 6254011

Abstract

The retention of the two parental mitochondrial DNAs has been investigated in a large number of mouse-human cell hybrids segregating either mouse or human chromosomes, by using a highly sensitive and specific method for detection of the DNA; the results have been correlated with the karyotype and isozyme marker pattern in the same hybrid lines. In all the hybrids examined, a consistent pattern was observed for the type of mitochondrial DNA retained: the mitochondrial DNA of the parent whose chromosomes were segregated from the nucleus was undetectable or present in marginal amounts. This was true also of hybrids containing a complete set of the segregating chromosomes in the total or a large fraction of the cell population.

Full text

PDF
4079

Images in this article

4080Image
on p.4080
4081Image
on p.4081
4081Image
on p.4081
4082Image
on p.4082
4082Image
on p.4082
4082Image
on p.4082
4083Image
on p.4083

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi B., Attardi G. Persistence of thymidine kinase activity in mitochondria of a thymidine kinase-deficient derivative of mouse L cells. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2874–2878. doi: 10.1073/pnas.69.10.2874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Attardi B., Cravioto B., Attardi G. Membrane-bound ribosomes in HeLa cells. I. Their proportion to total cell ribosomes and their association with messenger RNA. J Mol Biol. 1969 Aug 28;44(1):47–70. doi: 10.1016/0022-2836(69)90404-5. [DOI] [PubMed] [Google Scholar]
  3. Chern C. J., Croce C. M. Assignment of the structural gene for human beta glucuronidase to chromosome 7 and tetrameric association of subunits in the enzyme molecule. Am J Hum Genet. 1976 Jul;28(4):350–356. [PMC free article] [PubMed] [Google Scholar]
  4. Clayton D. A., Teplitz R. L., Nabholz M., Dovey H., Bodmer W. Mitochondrial DNA of human-mouse cell hybrids. Nature. 1971 Dec 31;234(5331):560–562. doi: 10.1038/234560a0. [DOI] [PubMed] [Google Scholar]
  5. Coon H. G., Horak I., Dawid I. B. Propagation of both parental mitochondrial DNAs in rat-human and mouse-human hybrid cells. J Mol Biol. 1973 Dec 15;81(3):285–298. doi: 10.1016/0022-2836(73)90142-3. [DOI] [PubMed] [Google Scholar]
  6. Croce C. M., Kieba I., Koprowski H. Unidirectional loss of human chromosomes in rat-human hybrids. Exp Cell Res. 1973 Jun;79(2):461–463. doi: 10.1016/0014-4827(73)90470-9. [DOI] [PubMed] [Google Scholar]
  7. Croce C. M. Loss of mouse chromosomes in somatic cell hybrids between HT-1080 human fibrosarcoma cells and mouse peritioneal macrophages. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3248–3252. doi: 10.1073/pnas.73.9.3248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Croce C. M., Talavera A., Basilico C., Miller O. J. Suppression of production of mouse 28S ribosomal RNA in mouse-human hybrids segregating mouse chromosomes. Proc Natl Acad Sci U S A. 1977 Feb;74(2):694–697. doi: 10.1073/pnas.74.2.694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eliceiri G. L., Green H. Ribosomal RNA synthesis in human-mouse hybrid cells. J Mol Biol. 1969 Apr;41(2):253–260. doi: 10.1016/0022-2836(69)90390-8. [DOI] [PubMed] [Google Scholar]
  10. Eliceiri G. L. Synthesis of mitochondrial RNA in hamster-mouse hybrid cells. Nat New Biol. 1973 Feb 21;241(112):233–234. doi: 10.1038/newbio241233a0. [DOI] [PubMed] [Google Scholar]
  11. Huebner K., Shander M., Croce C. M. Suppression of replication of SV40 and polyoma virus in mouse-human hybrids. Cell. 1977 May;11(1):25–33. doi: 10.1016/0092-8674(77)90314-2. [DOI] [PubMed] [Google Scholar]
  12. Jha K. K., Ozer H. L. Expression of transformation in cell hybrids. I. Isolation and application of density-inhibited Balb/3T3 cells deficient in hypoxanthine phosphoribosyltransferase and resistant to ouabain. Somatic Cell Genet. 1976 May;2(3):215–223. doi: 10.1007/BF01538960. [DOI] [PubMed] [Google Scholar]
  13. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  14. Marshall C. J. Synthesis of ribosomal RNA in synkaryons and heterokaryons formed between human and rodent cells. J Cell Sci. 1975 Mar;17(3):307–325. doi: 10.1242/jcs.17.3.307. [DOI] [PubMed] [Google Scholar]
  15. Miller O. J., Miller D. A., Dev V. G., Tantravahi R., Croce C. M. Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4531–4535. doi: 10.1073/pnas.73.12.4531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. O'Brien D., Linnenbach A., Croce C. M. Assignment of the gene for lactic dehydrogenase A to mouse chromosome 7 using mouse-human hybrids. Cytogenet Cell Genet. 1978;21(1-2):72–76. doi: 10.1159/000130879. [DOI] [PubMed] [Google Scholar]
  17. Ojala D., Attardi G. A detailed physical map of HeLa cell mitochondria DNA and its alignment with the positions of known genetic markers. Plasmid. 1977 Nov;1(1):78–105. doi: 10.1016/0147-619x(77)90010-5. [DOI] [PubMed] [Google Scholar]
  18. Parker R. C., Watson R. M. Restriction endonuclease cleavage maps of rat and mouse mitochondrial DNAs. Nucleic Acids Res. 1977;4(5):1291–1299. doi: 10.1093/nar/4.5.1291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Perry R. P., Kelley D. E., Schibler U., Huebner K., Croce C. M. Selective suppression of the transcription of ribosomal genes in mouse-human hybrid cells. J Cell Physiol. 1979 Mar;98(3):553–559. doi: 10.1002/jcp.1040980313. [DOI] [PubMed] [Google Scholar]
  20. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  21. Seabright M. A rapid banding technique for human chromosomes. Lancet. 1971 Oct 30;2(7731):971–972. doi: 10.1016/s0140-6736(71)90287-x. [DOI] [PubMed] [Google Scholar]
  22. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  23. Storrie B., Attardi G. Expression of the mitochondrial genome in HeLa cells. 13. Effect of selective inhibition of cytoplasmic or mitochondrial protein synthesis on mitochondrial nucleic acid synthesis. J Mol Biol. 1972 Nov 14;71(2):177–199. doi: 10.1016/0022-2836(72)90345-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES