Abstract
Two-dimensional gel electrophoresis has allowed the detection of proteins characteristic of inner cell mass and trophectoderm in mouse blastocyst. Certain of the proteins characterizing trophectoderm copurify with intermediate filaments from trophectoderm and a trophoblastoma cell line. A monoclonal antibody prepared against proteins of these intermediate filaments labels a filament network in trophectoderm but not in inner cell mass cells.
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- Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Copp A. J. Interaction between inner cell mass and trophectoderm of the mouse blastocyst. I. A study of cellular proliferation. J Embryol Exp Morphol. 1978 Dec;48:109–125. [PubMed] [Google Scholar]
- Dewey M. J., Filler R., Mintz B. Protein patterns of developmentally totipotent mouse teratocarcinoma cells and normal early embryo cells. Dev Biol. 1978 Jul;65(1):171–182. doi: 10.1016/0012-1606(78)90188-4. [DOI] [PubMed] [Google Scholar]
- Ducibella T., Ukena T., Karnovsky M., Anderson E. Changes in cell surface and cortical cytoplasmic organization during early embryogenesis in the preimplantation mouse embryo. J Cell Biol. 1977 Jul;74(1):153–167. doi: 10.1083/jcb.74.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franke W. W., Schmid E., Weber K., Osborn M. HeLa cells contain intermediate-sized filaments of the prekeratin type. Exp Cell Res. 1979 Jan;118(1):95–109. doi: 10.1016/0014-4827(79)90587-1. [DOI] [PubMed] [Google Scholar]
- GRAHAM R. C., Jr, LUNDHOLM U., KARNOVSKY M. J. CYTOCHEMICAL DEMONSTRATION OF PEROXIDASE ACTIVITY WITH 3-AMINO-9-ETHYLCARBAZOLE. J Histochem Cytochem. 1965 Feb;13:150–152. doi: 10.1177/13.2.150. [DOI] [PubMed] [Google Scholar]
- Garrels J. I. Two dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines. J Biol Chem. 1979 Aug 25;254(16):7961–7977. [PubMed] [Google Scholar]
- Handyside A. H., Johnson M. H. Temporal and spatial patterns of the synthesis of tissue-specific polypeptides in the preimplantation mouse embryo. J Embryol Exp Morphol. 1978 Apr;44:191–199. [PubMed] [Google Scholar]
- Howe C. C., Solter D. Cytoplasmic and nuclear protein synthesis in preimplantation mouse embryos. J Embryol Exp Morphol. 1979 Aug;52:209–225. [PubMed] [Google Scholar]
- Kemler R., Babinet C., Eisen H., Jacob F. Surface antigen in early differentiation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4449–4452. doi: 10.1073/pnas.74.10.4449. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
- Lazarides E. Intermediate filaments as mechanical integrators of cellular space. Nature. 1980 Jan 17;283(5744):249–256. doi: 10.1038/283249a0. [DOI] [PubMed] [Google Scholar]
- Lehman J. M., Speers W. C., Swartzendruber D. E., Pierce G. B. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma. J Cell Physiol. 1974 Aug;84(1):13–27. doi: 10.1002/jcp.1040840103. [DOI] [PubMed] [Google Scholar]
- Levinson J., Goodfellow P., vadeboncoeur M., McDevitt H. Identification of stage-specific polypeptides synthesized during murine preimplantation development. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3332–3336. doi: 10.1073/pnas.75.7.3332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin G. R., Evans M. J. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1441–1445. doi: 10.1073/pnas.72.4.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin G. R., Evans M. J. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1441–1445. doi: 10.1073/pnas.72.4.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nicolas J. F., Avner P., Gaillard J., Guenet J. L., Jakob H., Jacob F. Cell lines derived from teratocarcinomas. Cancer Res. 1976 Nov;36(11 Pt 2):4224–4231. [PubMed] [Google Scholar]
- Van Blerkom J., Barton S. C., Johnson M. H. Molecular differentiation in the preimplantation mouse embryo. Nature. 1976 Jan 29;259(5541):319–321. doi: 10.1038/259319a0. [DOI] [PubMed] [Google Scholar]