Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Aug;77(8):4454–4458. doi: 10.1073/pnas.77.8.4454

Genomic changes associated with antigenic variation of visna virus durig persistent infection.

J E Clements, F S Pedersen, O Narayan, W A Haseltine
PMCID: PMC349862  PMID: 6254026

Abstract

Visna virus undergoes antigenic change during persistent infection of sheep. Antigenic variants of visna virus were compared by using the genomic RNA and analyzing the large RNase T1-resistant oligonucleotides. Mutants isolated from a persistently infected sheep contained a small number of changes in their oligonucleotide patterns when compared with parental virus. To determine whether the changes in the nucleotide structure were clustered in one region of the genome, we determined the order of the oligonucleotides of the parental and mutant RNAs along the genome with respect to the 3' polyadenylylated end. All but one difference between the parental strain and the antigenic mutant used for mapping were located within 2 kilobases from the 3' terminus. Nucleotide sequence analyses showed that several of the oligonucleotides that differed in the parental and mutant RNAs could be accounted for by single base changes.

Full text

PDF
4454

Images in this article

4455Image
on p.4455
4457Image
on p.4457

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beemon K. L., Faras A. J., Hasse A. T., Duesberg P. H., Maisel J. E. Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses. J Virol. 1976 Feb;17(2):525–537. doi: 10.1128/jvi.17.2.525-537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clements J. E., Narayan O., Griffin D. E., Johnson R. T. The synthesis and structure of visna virus DNA. Virology. 1979 Mar;93(2):377–386. doi: 10.1016/0042-6822(79)90242-3. [DOI] [PubMed] [Google Scholar]
  3. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerhard W., Webster R. G. Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 (HON1) influenza virus with monoclonal antibodies. J Exp Med. 1978 Aug 1;148(2):383–392. doi: 10.1084/jem.148.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Haase A. T. The slow infection caused by visna virus. Curr Top Microbiol Immunol. 1975;72:101–156. doi: 10.1007/978-3-642-66289-8_4. [DOI] [PubMed] [Google Scholar]
  6. Ihle J. N., Lazar B. Natural immunity in mice to the envelope glycoprotein of endogenous ecotropic type C viruses: neutralization of virus infectivity. J Virol. 1977 Mar;21(3):974–980. doi: 10.1128/jvi.21.3.974-980.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Laver W. G., Gerhard W., Webster R. G., Frankel M. E., Air G. M. Antigenic drift in type A influenza virus: peptide mapping and antigenic analysis of A/PR/8/34 (HON1) variants selected with monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1425–1429. doi: 10.1073/pnas.76.3.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Narayan O., Griffin D. E., Chase J. Antigenic shift of visna virus in persistently infected sheep. Science. 1977 Jul 22;197(4301):376–378. doi: 10.1126/science.195339. [DOI] [PubMed] [Google Scholar]
  9. Narayan O., Griffin D. E., Clements J. E. Virus mutation during 'slow infection': temporal development and characterization of mutants of visna virus recovered from sheep. J Gen Virol. 1978 Nov;41(2):343–352. doi: 10.1099/0022-1317-41-2-343. [DOI] [PubMed] [Google Scholar]
  10. Pedersen F. S., Haseltine W. A. A micromethod for detailed characterization of high molecular weight RNA. Methods Enzymol. 1980;65(1):680–687. doi: 10.1016/s0076-6879(80)65066-6. [DOI] [PubMed] [Google Scholar]
  11. Pedersen F. S., Haseltine W. A. Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high-molecular-weight RNA. J Virol. 1980 Jan;33(1):349–365. doi: 10.1128/jvi.33.1.349-365.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SIGURDSSON B., PALSSON P. A. Visna of sheep; a slow, demyelinating infection. Br J Exp Pathol. 1958 Oct;39(5):519–528. [PMC free article] [PubMed] [Google Scholar]
  13. Scott J. V., Stowring L., Haase A. T., Narayan O., Vigne R. Antigenic variation in visna virus. Cell. 1979 Oct;18(2):321–327. doi: 10.1016/0092-8674(79)90051-5. [DOI] [PubMed] [Google Scholar]
  14. Steeves R. A., Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein. J Virol. 1974 Jul;14(1):187–189. doi: 10.1128/jvi.14.1.187-189.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vigne R., Brahic M., Filippi P., Tamalet J. Complexity and polyadenylic acid content of visna virus 60-70S RNA. J Virol. 1977 Jan;21(1):386–395. doi: 10.1128/jvi.21.1.386-395.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vigne R., Filippi P., Brahic M., Tamalet J. Absence of circularly permuted and largely redundant sequences in the genome of visna virus. J Virol. 1978 Nov;28(2):543–550. doi: 10.1128/jvi.28.2.543-550.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES