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. 1980 Aug;77(8):4529–4533. doi: 10.1073/pnas.77.8.4529

Ouabain-binding-site photoaffinity probes that label both subunits of Na+,K+-ATPase.

C Hall, A Ruoho
PMCID: PMC349877  PMID: 6254030

Abstract

4"'-Diazomalonyldigitoxin and its isomer, 3"'-diazomalonyldigitoxin, have been synthesized at high specific radioactivity and used as photolabels for the Na,K-ATPase (ATP phosphohydrolase, EC 3.6.1.3) purified from Electrophorus electricus. Photoaffinity labeling experiments using both type I and type II complexes of enzyme with both photolabels showed ouabain-protectable labeling of the alpha as well as the beta subunit. These data suggest that, in the purified eel enzyme, the alpha and beta subunits are in intimate contact, at least in the region of the third digitoxose of the "sugar-specific" binding site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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