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. 1980 Aug;77(8):4662–4665. doi: 10.1073/pnas.77.8.4662

Polycistronic vesicular stomatitis virus RNA transcripts.

R C Herman, M Schubert, J D Keene, R A Lazzarini
PMCID: PMC349905  PMID: 6254036

Abstract

A procedure to enrich for the sequences present at the junction between the linked messages in the polycistronic RNAs symthesized in vitro by vesicular stomatitis virus (VSV) is described. Analyses of these sequences show that they contain a precise transcript of both the intercistronic dinucleotide and the pentanucleotide 5'--C-U-G-U-U--3', common to the 5'-end of all VSV cistrons, covalently linked to the 3'-side of the intervening poly(A). The data strongly suggest that the VSV transcriptase polyadenylylates the mRNAs and can then resume direct and precise transcription of the genome-without reinitiation and without skipping nucleotides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banerjee A. D., Abraham G., Colonno R. J. Vesicular stomatitis virus: mode of transcription. J Gen Virol. 1977 Jan;34(1):1–8. doi: 10.1099/0022-1317-34-1-1. [DOI] [PubMed] [Google Scholar]
  2. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  3. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Herman R. C., Adler S., Lazzarini R. A., Colonno R. J., Banerjee A. K., Westphal H. Intervening polyadenylate sequences in RNA transcripts of vesicular stomatitis virus. Cell. 1978 Oct;15(2):587–596. doi: 10.1016/0092-8674(78)90027-2. [DOI] [PubMed] [Google Scholar]
  5. Keene J. D., Schubert M., Lazzarini R. A. Intervening sequence between the leader region and the nucleopcapsid gene of vesicular stomatitis virus RNA. J Virol. 1980 Feb;33(2):789–794. doi: 10.1128/jvi.33.2.789-794.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Keller W., Crouch R. Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360–3364. doi: 10.1073/pnas.69.11.3360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lazzarini R. A., Weber G. H., Johnson L. D., Stamminger G. M. Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle. J Mol Biol. 1975 Sep 25;97(3):289–307. doi: 10.1016/s0022-2836(75)80042-8. [DOI] [PubMed] [Google Scholar]
  8. Marshall S., Gillespie D. Poly U tracts absent from viral RNA. Nat New Biol. 1972 Nov 8;240(97):43–45. doi: 10.1038/newbio240043a0. [DOI] [PubMed] [Google Scholar]
  9. McGeoch D. J. Structure of the gene N:gene NS intercistronic junction in the genome of vesicular stomatitis virus. Cell. 1979 Jul;17(3):673–681. doi: 10.1016/0092-8674(79)90274-5. [DOI] [PubMed] [Google Scholar]
  10. McGeoch D. J., Turnbull N. T. Analysis of the 3'-terminal nucleotide sequence of vesicular stomatitis virus N protein mRNA. Nucleic Acids Res. 1978 Nov;5(11):4007–4024. doi: 10.1093/nar/5.11.4007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy M. F., Lazzarini R. A. Synthesis of viral mRNA and polyadenylate by a ribonucleoprotein complex from extracts of VSV-infected cells. Cell. 1974 Sep;3(1):77–84. doi: 10.1016/0092-8674(74)90043-9. [DOI] [PubMed] [Google Scholar]
  12. Petric M., Prevec L. Vesicular stomatitis virus--a new interfering particle, intracellular structures, and virus-specific RNA. Virology. 1970 Aug;41(4):615–630. doi: 10.1016/0042-6822(70)90427-7. [DOI] [PubMed] [Google Scholar]
  13. Rhodes D. P., Banerjee A. K. 5'-terminal sequence of vesicular stomatitis virus mRNA's synthesized in vitro. J Virol. 1975 Jan;17(1):33–42. doi: 10.1128/jvi.17.1.33-42.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rose J. K. Complete intergenic and flanking gene sequences from the genome of vesicular stomatitis virus. Cell. 1980 Feb;19(2):415–421. doi: 10.1016/0092-8674(80)90515-2. [DOI] [PubMed] [Google Scholar]
  15. Rose J. K., Iverson L. Nucleotide sequences from the 3'-ends of vesicular stomatitis virus mRNA's as determined from cloned DNA. J Virol. 1979 Nov;32(2):404–411. doi: 10.1128/jvi.32.2.404-411.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rose J. K., Lodish H. F., Brock M. L. Giant heterogeneous polyadenylic acid on vesicular stomatitis virus mRNA synthesized in vitro in the presence of S-adenosylhomocysteine. J Virol. 1977 Feb;21(2):683–693. doi: 10.1128/jvi.21.2.683-693.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rowlands D. J. Sequences of vesicular stomatitis virus RNA in the region coding for leader RNA, N protein mRNA, and their junction. Proc Natl Acad Sci U S A. 1979 Oct;76(10):4793–4797. doi: 10.1073/pnas.76.10.4793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sanger F., Brownlee G. G., Barrell B. G. A two-dimensional fractionation procedure for radioactive nucleotides. J Mol Biol. 1965 Sep;13(2):373–398. doi: 10.1016/s0022-2836(65)80104-8. [DOI] [PubMed] [Google Scholar]
  19. Schubert M., Keene J. D., Herman R. C., Lazzarini R. A. Site on the vesicular stomatitis virus genome specifying polyadenylation and the end of the L gene mRNA. J Virol. 1980 May;34(2):550–559. doi: 10.1128/jvi.34.2.550-559.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schubert M., Keene J. D., Lazzarini R. A. A specific internal RNA polymerase recognition site of VSV RNA is involved in the generation of DI particles. Cell. 1979 Nov;18(3):749–757. doi: 10.1016/0092-8674(79)90128-4. [DOI] [PubMed] [Google Scholar]
  21. Schubert M., Keene J. D., Lazzarini R. A., Emerson S. U. The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell. 1978 Sep;15(1):103–112. doi: 10.1016/0092-8674(78)90086-7. [DOI] [PubMed] [Google Scholar]
  22. Testa D., Banerjee A. K. In vitro synthesis of a possible precursor RNA by purified vesicular stomatitis virus. Biochem Biophys Res Commun. 1978 May 30;82(2):655–664. doi: 10.1016/0006-291x(78)90925-7. [DOI] [PubMed] [Google Scholar]

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