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. 1980 Aug;77(8):4788–4792. doi: 10.1073/pnas.77.8.4788

The cbpA gene: role of the 26,000-dalton carbohydrate-binding protein in intercellular cohesion of developing Dictyostelium discoideum cells.

T M Shinnick, R A Lerner
PMCID: PMC349932  PMID: 6933527

Abstract

The loss of the lectin-like activity of a 26,000-dalton carbohydrate-binding protein (CBP-26) results in the loss of aggregation competence and cell-cell cohesiveness in developing cells of Dictyostelium discoideum. The lesion responsible for this phenotype behaves like a mutation in the structural gene for CBP-26, maps in linkage group II, and has been designated cbpA1. In aggregation-competent revertants, the degree of aggregation competence and cell-cell cohesiveness is directly related to the specific activity of CBP-26. Thus, CBP-26 appears to play an essential role in cell aggregation through the cell-cell cohesion process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beug H., Katz F. E., Gerisch G. Dynamics of antigenic membrane sites relating to cell aggregation in Dictyostelium discoideum. J Cell Biol. 1973 Mar;56(3):647–658. doi: 10.1083/jcb.56.3.647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang C. M., Reitherman R. W., Rosen S. D., Barondes S. H. Cell surface location of discoidin, a developmentally regulated carbohydrate-binding protein from Dictyostelium discoideum. Exp Cell Res. 1975 Oct 1;95(1):136–142. doi: 10.1016/0014-4827(75)90618-7. [DOI] [PubMed] [Google Scholar]
  3. Free S. J., Schimke R. T. The structural gene for alpha-mannosidase-1 in Dictyostellium discoideum. Genetics. 1976 Oct;84(2):159–174. doi: 10.1093/genetics/84.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Geltosky J. E., Weseman J., Bakke A., Lerner R. A. Identification of a cell surface glycoprotein involved in cell aggregation in D. discoideum. Cell. 1979 Oct;18(2):391–398. doi: 10.1016/0092-8674(79)90058-8. [DOI] [PubMed] [Google Scholar]
  5. Katz E. R., Sussman M. Parasexual recombination in Dictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants (clones-temperature sensitive-ploidy-fruiting bodies-spore-slime mold). Proc Natl Acad Sci U S A. 1972 Feb;69(2):495–498. doi: 10.1073/pnas.69.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Loomis W. F., Jr Temperature-sensitive mutants of Dictyostelium discoideum. J Bacteriol. 1969 Jul;99(1):65–69. doi: 10.1128/jb.99.1.65-69.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ray J., Shinnick T., Lerner R. A mutation altering the function of a carbohydrate binding protein blocks cell-cell cohesion in developing Dictyostelium discoideum. Nature. 1979 May 17;279(5710):215–221. doi: 10.1038/279215a0. [DOI] [PubMed] [Google Scholar]
  8. Reitherman R. W., Rosen S. D., Frasier W. A., Barondes S. H. Cell surface species-specific high affinity receptors for discoidin: developmental regulation in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3541–3545. doi: 10.1073/pnas.72.9.3541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosen S. D., Kafka J. A., Simpson D. L., Barondes S. H. Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2554–2557. doi: 10.1073/pnas.70.9.2554. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rosen S. D., Reitherman R. W., Barondes S. H. Distinct lectin activities from six species of cellular slime molds. Exp Cell Res. 1975 Oct 1;95(1):159–166. doi: 10.1016/0014-4827(75)90621-7. [DOI] [PubMed] [Google Scholar]
  11. SUSSMAN M. Synergistic and antagonistic interactions between morphogenetically deficient variants of the slime mould Dictyostelium discoideum. J Gen Microbiol. 1954 Feb;10(1):110–120. doi: 10.1099/00221287-10-1-110. [DOI] [PubMed] [Google Scholar]
  12. Siu C. H., Lerner R. A., Ma G., Firtel R. A., Loomis W. F. Developmentally regulated proteins of the plasma membrane of Dictyostelium discoideum. The carbohydrate-binding protein. J Mol Biol. 1976 Jan 15;100(2):157–178. doi: 10.1016/s0022-2836(76)80146-5. [DOI] [PubMed] [Google Scholar]
  13. Williams K. L., Kessin R. H., Newell P. C. Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. J Gen Microbiol. 1974 Sep;84(1):59–69. doi: 10.1099/00221287-84-1-59. [DOI] [PubMed] [Google Scholar]

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