Figure 2. Venn diagram illustrating overlapping deregulated genes between cancer cell lines and cholangiocarcinomas. Using microarray analyses, a total of 654 genes were upregulated in CCA cell lines after epigenetic drug treatment (red circle, “cell lines - present study”). Sixty (white numbers in bold; Table S2) of these were simultaneously downregulated in at least one previously published data set of tumor compared with non-malignant controls (Miller et al.²⁵ and Obama et al.²⁶). ICC, intrahepatic cholangiocarcinoma; ECC, extrahepatic cholangiocarcinoma.