Figure 4. Antibody-dependent cytokine release induced by MB314 or MB311. Antibody dependent cytokine release was measured in parallel to the antibody-dependent cell lysis assay. SKBR-3 target cells and effector cells (PBMCs from a healthy volunteer donor) at an E:T ratio of 25:1 were incubated with different concentrations of MB311 or MB314, respectively, for 3 h. Supernatants were analyzed for release of cytokines using xMAP multiplex technology. Release of TNFα (A, B), MCP-1 (C, D), IL-6 (E, F) and IFNγ (G, H) are shown. Mean and SD of triplicates are shown. Competition assay: Target and effector cells were incubated with the highest concentrations of MB314 or MB311 (400 ng/ml and 4 mg/ml, respectively) in the absence or presence of excess of human IgG.