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. 1980 Sep;77(9):5105–5109. doi: 10.1073/pnas.77.9.5105

Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands.

M D Challberg, S V Desiderio, T J Kelly Jr
PMCID: PMC350005  PMID: 6933548

Abstract

The 5' terminus of each nascent daughter strand of adenovirus DNA replicated in vitro is covalently linked to a protein with an apparent Mr of 80,000. This protein may represent a precursor to the 55,000-dalton protein known to be linked to the 5' ends of mature adenovirus DNA strands. Partial proteolysis experiments indicate that the 80,000-dalton and 55,000-dalton proteins are structurally related. Furthermore, both proteins are attached to DNA by the same linkage: a phosphodiester bond between the beta-OH of a serine residue in the protein and the 5'-OH of the terminal deoxycytidine residue of the DNA. The role of the 80,000-dalton protein in adenovirus DNA replication is not yet clear, although one reasonable possibility is that it serves as the primer for daughter strand synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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