Abstract
We have obtained the electron paramagnetic resonance spectrum of Cu2+ bound in a tetragonal single crystal of hen egg-white lysozyme. A part of this spectrum has been shown to originate from Cu2+ ions bound at the site designated as B by Teichberg et al. [Teichberg, V.I., Sharon, N., Moult, J., Smilansky, A. & Yonath, A. (1974) J. Mol. Biol. 87, 357-368]. The values of the spin hamiltonian parameters that describe this part of the spectrum are reported. The implications of these values with respect to the chemical nature and configuration of ligands are discussed. The other features of the spectrum are also described.
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Selected References
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- Blake C. C., Koenig D. F., Mair G. A., North A. C., Phillips D. C., Sarma V. R. Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution. Nature. 1965 May 22;206(4986):757–761. doi: 10.1038/206757a0. [DOI] [PubMed] [Google Scholar]
- Diamond R. Real-space refinement of the structure of hen egg-white lysozyme. J Mol Biol. 1974 Jan 25;82(3):371–391. doi: 10.1016/0022-2836(74)90598-1. [DOI] [PubMed] [Google Scholar]
- Douzou P., Hoa G. H., Petsko G. A. Protein crystallography at sub-zero temperatures: lysozyme-substrate complexes in cooled mixed solvents. J Mol Biol. 1975 Aug 15;96(3):367–380. doi: 10.1016/0022-2836(75)90166-7. [DOI] [PubMed] [Google Scholar]
- Haas D. J. Preliminary studies on the denaturation of cross-linked lysozyme crystals. Biophys J. 1968 May;8(5):549–555. doi: 10.1016/S0006-3495(68)86507-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hui-Bon-Hoa G., Douzou P. Ionic strength and protonic activity of supercooled solutions used in experiments with enzyme systems. J Biol Chem. 1973 Jul 10;248(13):4649–4654. [PubMed] [Google Scholar]
- Petsko G. A. Protein crystallography at sub-zero temperatures: cryo-protective mother liquors for protein crystals. J Mol Biol. 1975 Aug 15;96(3):381–392. doi: 10.1016/0022-2836(75)90167-9. [DOI] [PubMed] [Google Scholar]
- Teichberg V. I., Sharon N., Moult J., Smilansky A., Yonath A. Binding of divalent copper ions to aspartic acid residue 52 in hen egg-white lysozyme. J Mol Biol. 1974 Aug 5;87(2):357–368. doi: 10.1016/0022-2836(74)90155-7. [DOI] [PubMed] [Google Scholar]
- Yonath A., Sielecki A., Moult J., Podjarny A., Traub W. Crystallographic studies of protein denaturation and renaturation. 1. Effects of denaturants on volume and X-ray pattern of cross-linked triclinic lysozyme crystals. Biochemistry. 1977 Apr 5;16(7):1413–1417. doi: 10.1021/bi00626a027. [DOI] [PubMed] [Google Scholar]
