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. 1980 Sep;77(9):5432–5436. doi: 10.1073/pnas.77.9.5432

Development and characterization of allospecific long-term human cytolytic T-cell lines.

C S Reiss, M E Hemler, V H Englehard, J W Mier, J L Strominger, S J Burakoff
PMCID: PMC350073  PMID: 6968913

Abstract

Two long-term human cytolytic T lymphocyte (CTL) lines (VE and JR), whose cytolytic activity was dependent upon both irradiated JY cells (the stimulating alloantigen) and T-cell growth factor, were established. These lines were monitored in culture for 6-8 months. Both lines were specific for HLA-A or B antigens or both and the JR line was allospecific for HLA-B7. These CTL lines killed specific target cells at an effector-to-target ratio of 0.4 (VE) or 0.08 (JR). All of the cells, which grow in suspension, rosetted with sheep erythrocytes and reacted with an antiserum specific for human T cells. The CTL line VE was used to raise rabbit antisera that immunoprecipitated two specific polypeptides (78,000 and 33,000 daltons) from labeled membranes of these CTL lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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