Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Sep;77(9):5453–5457. doi: 10.1073/pnas.77.9.5453

Transfer of Epstein-Barr virus receptors to receptor-negative cells permits virus penetration and antigen expression.

D J Volsky, I M Shapiro, G Klein
PMCID: PMC350078  PMID: 6254082

Abstract

Epstein-Barr virus (EBV) receptors were implanted into the membranes of receptor-negative cells, using Sendai virus envelopes as vehicles. The presence of the receptors in the target cell membrane was demonstrated by monitoring the fate of radioiodinated donor membranes. Receptors could be detected for at least 36 hr after implantation. [3H]Thymidine-labeled EBV bound efficiently to receptor-implanted cells but not to control cells. Binding was inhibited by an excess of nonlabeled virus. Of the [3H]thymidine-labeled EBV DNA, 50-75% was found inside the receptor-implanted, EBV-exposed cells 24 hr after the infection. The viral genome was functionally active in B lymphocyte-derived cell lines of human, murine, and baboon origin; in T lymphocyte-derived lines of human and murine origin; in mouse fibroblasts; and in freshly explanted mouse lymphocytes, as shown by the expression of EBV-determined nuclear, early, and viral capsid antigens.

Full text

PDF
5453

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Einhorn L., Steinitz M., Yefenof E., Ernberg I., Bakacs T., Klein G. Epstein-Barr virus (EBV) receptors, complement receptors, and EBV infectibility of different lymphocyte fractions of human peripheral blood. II. Epstein-Barr virus studies. Cell Immunol. 1978 Jan;35(1):43–58. doi: 10.1016/0008-8749(78)90125-9. [DOI] [PubMed] [Google Scholar]
  2. Falk L., Lindahl T., Bjursell G., Klein G. Herpesvirus papio: state and properties of intracellular viral DNA in baboon lymphoblastoid cell lines. Int J Cancer. 1979 Jul 15;24(1):75–79. doi: 10.1002/ijc.2910240113. [DOI] [PubMed] [Google Scholar]
  3. Graessmann A., Wolf H., Bornkamm G. W. Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA. Proc Natl Acad Sci U S A. 1980 Jan;77(1):433–436. doi: 10.1073/pnas.77.1.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Greaves M. F., Brown G. Epstein-Barr virus binding sites on lymphocyte subpopulations and the origin of lymphoblasts in cultured lymphoic cell lines and in the blood of patients with infectious mononucleosis. Clin Immunol Immunopathol. 1975 Mar;3(4):514–524. doi: 10.1016/0090-1229(75)90076-8. [DOI] [PubMed] [Google Scholar]
  5. Hinuma Y., Grace J. T., Jr Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc Soc Exp Biol Med. 1967 Jan;124(1):107–111. doi: 10.3181/00379727-124-31677. [DOI] [PubMed] [Google Scholar]
  6. Jondal M., Klein G., Oldstone M. B., Bokish V., Yefenof E. Surface markers on human B and T lymphocytes. VIII. Association between complement and Epstein-Barr virus receptors on human lymphoid cells. Scand J Immunol. 1976;5(4):401–410. doi: 10.1111/j.1365-3083.1976.tb00294.x. [DOI] [PubMed] [Google Scholar]
  7. Jondal M., Klein G. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J Exp Med. 1973 Dec 1;138(6):1365–1378. doi: 10.1084/jem.138.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Klein G., Dombos L., Gothoskar B. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int J Cancer. 1972 Jul 15;10(1):44–57. doi: 10.1002/ijc.2910100108. [DOI] [PubMed] [Google Scholar]
  9. Klein G. The Epstein-Barr virus and neoplasia. N Engl J Med. 1975 Dec 25;293(26):1353–1357. doi: 10.1056/NEJM197512252932607. [DOI] [PubMed] [Google Scholar]
  10. Klein G., Yefenof E., Falk K., Westman A. Relationship between Epstein-Barr virus (EBV)-production and the loss of the EBV receptor/complement receptor complex in a series of sublines derived from the same original Burkitt's lymphoma. Int J Cancer. 1978 May 15;21(5):552–560. doi: 10.1002/ijc.2910210504. [DOI] [PubMed] [Google Scholar]
  11. Klein G., Zeuthen J., Eriksson I., Terasaki P., Bernoco M., Rosén A., Masucci G., Povey S., Ber R. Hybridization of a myeloid leukemia-derived human cell line (K562) with a human Burkitt's lymphoma line (P3HR-1). J Natl Cancer Inst. 1980 Apr;64(4):725–738. [PubMed] [Google Scholar]
  12. Lopes J., Nachbar M., Zucker-Franklin D., Silber R. Lymphocyte plasma membranes: analysis of proteins and glycoproteins by SDS-gel electrophoresis. Blood. 1973 Jan;41(1):131–140. [PubMed] [Google Scholar]
  13. Menezes J., Leibold W., Klein G. Biological differences between Epstein-Barr virus (EBV) strains with regard to lymphocyte transforming ability, superinfection and antigen induction. Exp Cell Res. 1975 May;92(2):478–484. doi: 10.1016/0014-4827(75)90404-8. [DOI] [PubMed] [Google Scholar]
  14. Menezes J., Seigneurin J. M., Patel P., Bourkas A., Lenoir G. Presence of Epstein-Barr virus receptors, but absence of virus penetration, in cells of an Epstein-Barr virus genome-negative human lymphoblastoid T line (Molt 4). J Virol. 1977 Jun;22(3):816–821. doi: 10.1128/jvi.22.3.816-821.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller G., Lipman M. Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc Natl Acad Sci U S A. 1973 Jan;70(1):190–194. doi: 10.1073/pnas.70.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  17. Volsky D. J., Cabantchik Z. I., Beigel M., Loyter A. Implantation of the isolated human erythrocyte anion channel into plasma membranes of Friend erythroleukemic cells by use of Sendai virus envelopes. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5440–5444. doi: 10.1073/pnas.76.11.5440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Volsky D. J., Loyter A. An efficient method for reassembly of fusogenic Sendai virus envelopes after solubilization of intact virions with Triton X-100. FEBS Lett. 1978 Aug 15;92(2):190–194. doi: 10.1016/0014-5793(78)80751-0. [DOI] [PubMed] [Google Scholar]
  19. Willinger M., Frankel F. R. Fate of surface proteins of rabbit polymorphonuclear leukocytes during phagocytosis. I. Identification of surface proteins. J Cell Biol. 1979 Jul;82(1):32–44. doi: 10.1083/jcb.82.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yefenof E., Bakacs T., Einhorn L., Ernberg I., Klein G. Epstein-Barr virus (EBV) receptors, complement receptors, and EBV infectibility of different lymphocyte fractions of human peripheral blood. I. Complement receptor distribution and complement binding by separated lymphocyte subpopulations. Cell Immunol. 1978 Jan;35(1):34–42. doi: 10.1016/0008-8749(78)90124-7. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES