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. Author manuscript; available in PMC: 2013 Nov 12.

Published in final edited form as: Biomacromolecules. 2012 Oct 11;13(11):3841–3849. doi: 10.1021/bm301294n

Specific siRNA-mediated knockdown of GAPDH in MSCs at a variety of siRNA concentrations and charge ratios delivered via polymer diblock or DharmaFECT carriers. (A) MSCs were treated with siRNA at different concentrations with charge ratio of 4:1 (diblock copolymer carrier) or by manufacturer’s recommendations (DharmaFECT) and after 48 hours analyzed for GAPDH gene expression using RT-PCR with β-actin as the housekeeping gene (see Table 1 for primer sequences used). (B) MSCs were treated with GAPDH siRNA at 37.5 nM with charge ratios of 1:1–8:1 using the diblock copolymer delivery system. Data are from three independent experiments conducted in triplicate relative to gene expression of untreated cells (No Treatment) with error bars representing standard deviation. Statistical significance was evaluated at a level of p<0.05 using one-way analysis of variance. All pairwise comparisons were found significant unless noted by NS (not significant).

Specific siRNA-mediated knockdown of GAPDH in MSCs at a variety of siRNA concentrations and charge ratios delivered via polymer diblock or DharmaFECT carriers. (A) MSCs were treated with siRNA at different concentrations with charge ratio of 4:1 (diblock copolymer carrier) or by manufacturer’s recommendations (DharmaFECT) and after 48 hours analyzed for GAPDH gene expression using RT-PCR with β-actin as the housekeeping gene (see Table 1 for primer sequences used). (B) MSCs were treated with GAPDH siRNA at 37.5 nM with charge ratios of 1:1–8:1 using the diblock copolymer delivery system. Data are from three independent experiments conducted in triplicate relative to gene expression of untreated cells (No Treatment) with error bars representing standard deviation. Statistical significance was evaluated at a level of p<0.05 using one-way analysis of variance. All pairwise comparisons were found significant unless noted by NS (not significant).