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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Oct;77(10):5654–5658. doi: 10.1073/pnas.77.10.5654

Parathyroid hormone alters collagen synthesis and procollagen mRNA levels in fetal rat calvaria.

B E Kream, D W Rowe, S C Gworek, L G Raisz
PMCID: PMC350127  PMID: 6934501

Abstract

Parathyroid hormone decreased the incorporation of [3H]proline into collagenase-digestible protein in cultured 21-day fetal rat calvaria but had little effect on the labeling of noncollagen protein. After 24 hr of culture, there was a 50% reduction in collaghen synthesis and a 40% decrease in the level of functional procollagen mRNA as measured in a reticulocyte lystate translation assay. The effect of parathyroid hormone on both parameters was detectable after 6 hr of treatment. In these cultures, there was also a substantial degradation or release of newly synthesized collagen from the calvaria, but parathyroid hormone had little effect on the release of collagen into the medium. These results suggest that parathyroid hormone inhibits collagen synthesis primarily by decreasing the steady-state level of procollagen mRNA.

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Selected References

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  1. Bienkowski R. S., Cowan M. J., McDonald J. A., Crystal R. G. Degradation of newly synthesized collagen. J Biol Chem. 1978 Jun 25;253(12):4356–4363. [PubMed] [Google Scholar]
  2. Bingham P. J., Brazell I. A., Owen M. The effect of parathyroid extract on cellular activity and plasma calcium levels in vivo. J Endocrinol. 1969 Nov;45(3):387–400. doi: 10.1677/joe.0.0450387. [DOI] [PubMed] [Google Scholar]
  3. Bingham P. J., Raisz L. G. Bone growth in organ culture: effects of phosphate and other nutrients on bone and cartilage. Calcif Tissue Res. 1974;14(1):31–48. doi: 10.1007/BF02060281. [DOI] [PubMed] [Google Scholar]
  4. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  5. Dietrich J. W., Canalis E. M., Maina D. M., Raisz L. G. Hormonal control of bone collagen synthesis in vitro: effects of parathyroid hormone and calcitonin. Endocrinology. 1976 Apr;98(4):943–949. doi: 10.1210/endo-98-4-943. [DOI] [PubMed] [Google Scholar]
  6. FLANAGAN B., NICHOL S. G., Jr PARATHYROID INHIBITION OF BONE COLLAGEN SYNTHESIS. Endocrinology. 1964 Feb;74:180–186. doi: 10.1210/endo-74-2-180. [DOI] [PubMed] [Google Scholar]
  7. Fujimoto W. Y., Teague J., Williams R. H. Fibroblast monolayer cultures in scintillation counting vials: metabolic and growth experiments using radioisotopes and a microfluoremetric DNA assay. In Vitro. 1977 Apr;13(4):237–244. doi: 10.1007/BF02615081. [DOI] [PubMed] [Google Scholar]
  8. Guyette W. A., Matusik R. J., Rosen J. M. Prolactin-mediated transcriptional and post-transcriptional control of casein gene expression. Cell. 1979 Aug;17(4):1013–1023. doi: 10.1016/0092-8674(79)90340-4. [DOI] [PubMed] [Google Scholar]
  9. JOHNSTON C. C., Jr, DEISS W. P., Jr, MINER E. B. Bone matrix biosynthesis in vitro. II. Effects of parathyroid hormone. J Biol Chem. 1962 Nov;237:3560–3565. [PubMed] [Google Scholar]
  10. Jones S. J., Boyde A. Experimental study of changes in osteoblastic shape induced by calcitonin and parathyroid extract in an organ culture system. Cell Tissue Res. 1976 Jul 6;169(4):499–465. doi: 10.1007/BF00218146. [DOI] [PubMed] [Google Scholar]
  11. Kalu D. N., Doyle F. H., Pennock J., Foster G. V. Parathyroid hormone and experimental osteosclerosis. Lancet. 1970 Jun 27;1(7661):1363–1366. doi: 10.1016/s0140-6736(70)91271-7. [DOI] [PubMed] [Google Scholar]
  12. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  13. Matusik R. J., Rosen J. M. Prolactin induction of casein mRNA in organ culture. A model system for studying peptide hormone regulation of gene expression. J Biol Chem. 1978 Apr 10;253(7):2343–2347. [PubMed] [Google Scholar]
  14. Moen R. C., Rowe D. W., Palmiter R. D. Regulation of procollagen synthesis during the development of chick embryo calvaria. Correlation with procollagen mRNA content. J Biol Chem. 1979 May 10;254(9):3526–3530. [PubMed] [Google Scholar]
  15. Palmiter R. D. Ovalbumin messenger ribonucleic acid translation. Comparable rates of polypeptide initiation and elongation on ovalbumin and globin messenger ribonucleic acid in a rabbit reticulocyte lysate. J Biol Chem. 1973 Mar 25;248(6):2095–2106. [PubMed] [Google Scholar]
  16. Peterkofsky B., Diegelmann R. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971 Mar 16;10(6):988–994. doi: 10.1021/bi00782a009. [DOI] [PubMed] [Google Scholar]
  17. Peterkofsky B. The effect of ascorbic acid on collagen polypeptide synthesis and proline hydroxylation during the growth of cultured fibroblasts. Arch Biochem Biophys. 1972 Sep;152(1):318–328. doi: 10.1016/0003-9861(72)90221-4. [DOI] [PubMed] [Google Scholar]
  18. RAISZ L. G. BONE RESORPTION IN TISSUE CULTURE. FACTORS INFLUENCING THE RESPONSE TO PARATHYROID HORMONE. J Clin Invest. 1965 Jan;44:103–116. doi: 10.1172/JCI105117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Raisz L. G., Canalis E. M., Dietrich J. W., Kream B. E., Gworek S. C. Hormonal regulation of bone formation. Recent Prog Horm Res. 1978;34:335–356. doi: 10.1016/b978-0-12-571134-0.50013-2. [DOI] [PubMed] [Google Scholar]
  20. Reeve J., Hesp R., Williams D., Hulme P., Klenerman L., Zanelli J. M., Darby A. J., Tregear G. W., Parsons J. A. Anabolic effect of low doses of a fragment of human parathyroid hormone on the skeleton in postmenopausal osteoporosis. Lancet. 1976 May 15;1(7968):1035–1038. doi: 10.1016/s0140-6736(76)92216-9. [DOI] [PubMed] [Google Scholar]
  21. Rowe D. W., Moen R. C., Davidson J. M., Byers P. H., Bornstein P., Palmiter R. D. Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesis. Biochemistry. 1978 May 2;17(9):1581–1590. doi: 10.1021/bi00602a001. [DOI] [PubMed] [Google Scholar]
  22. Wong G. L., Luben R. A., Cohn D. V. 1,25-dihydroxycholecalciferol and parathormone: effects on isolated osteoclast-like and osteoblast-like cells. Science. 1977 Aug 12;197(4304):663–665. doi: 10.1126/science.195343. [DOI] [PubMed] [Google Scholar]

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