Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Oct;77(10):5711–5715. doi: 10.1073/pnas.77.10.5711

Adrenal glucocorticoid permissive regulation of muscle glycogenolysis: action on protein phosphatase(s) and its inhibitor(s).

G A Green, M Chenoweth, A Dunn
PMCID: PMC350139  PMID: 6255461

Abstract

Adrenal glucocorticoids exert a permissive action on the glycogen phosphorylase cascade. Epinephrine activation of muscle phosphorylase and phosphorylase b kinase is depressed in adrenalectomized rats. Phosphorylase phosphatase activity is increased by steroid lack, and normal epinephrine inhibition of the enzyme does not occur. Phosphorylase b kinase phosphatase activity is also increased; epinephrine, however, does not inhibit activity in muscle from normal or adrenalectomized rats. Protein phosphatase inhibitor activity is depressed in boiled dialyzed preparations made from adrenalectomized rat muscle. Cortisol resplacement therapy restores protein phosphatase inhibitor activity, decreases increased protein phosphatase activity, and restores normal epinephrine-induced activation of phosphorylase b kinase and phosphorylase and epinephrine inhibition of phosphorylase phosphatase.

Full text

PDF
5711

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Antoniw J. F., Cohen P. Separation of two phosphorylase kinase phosphatases from rabbit skeletal muscle. Eur J Biochem. 1976 Sep;68(1):45–54. doi: 10.1111/j.1432-1033.1976.tb10763.x. [DOI] [PubMed] [Google Scholar]
  2. Brandt H., Killilea S. D., Lee E. Y. Activation of phosphorylase phosphatase by a novel procedure: evidence for a regulatory mechanism involving the release of a catalytic subunit from enxyme-inhibitor complex(es) of higher molecular weight. Biochem Biophys Res Commun. 1974 Nov 27;61(2):598–604. doi: 10.1016/0006-291x(74)90999-1. [DOI] [PubMed] [Google Scholar]
  3. Brandt H., Lee E. Y., Killilea S. D. A protein inhibitor of rabbit liver phosphorylase phosphatase. Biochem Biophys Res Commun. 1975 Apr 21;63(4):950–956. doi: 10.1016/0006-291x(75)90661-0. [DOI] [PubMed] [Google Scholar]
  4. Chan T. M., Steiner K. E., Exton J. H. Effects of adrenalectomy on hormone action on hepatic glucose metabolism. Impaired glucagon activation of glycogen phosphorylase in hepatocytes from adrenalectomized rats. J Biol Chem. 1979 Nov 25;254(22):11374–11378. [PubMed] [Google Scholar]
  5. Chelala C. A., Torres H. N. Regulation of skeletal muscle phosphorylase phosphatase activity. II. Interconversions. Biochim Biophys Acta. 1970 Mar 18;198(3):504–513. doi: 10.1016/0005-2744(70)90128-2. [DOI] [PubMed] [Google Scholar]
  6. Cohen P., Antoniw J. F. The control of phosphorylase kinase phosphatase by "second site phosphorylation"; a new form of enzyme regulation. FEBS Lett. 1973 Aug 1;34(1):43–47. doi: 10.1016/0014-5793(73)80699-4. [DOI] [PubMed] [Google Scholar]
  7. Exton J. H., Friedmann N., Wong E. H., Brineaux J. P., Corbin J. D., Park C. R. Interaction of glucocorticoids with glucagon and epinephrine in the control of gluconeogenesis and glycogenolysis in liver and of lipolysis in adipose tissue. J Biol Chem. 1972 Jun 10;247(11):3579–3588. [PubMed] [Google Scholar]
  8. Gilboe D. P., Larson K. L., Nuttall F. Q. Radioactive method for the assay of glycogen phosphorylases. Anal Biochem. 1972 May;47(1):20–27. doi: 10.1016/0003-2697(72)90274-6. [DOI] [PubMed] [Google Scholar]
  9. Huang F. L., Glinsmann W. H. Inactivation of rabbit muscle phosphorylase phosphatase by cyclic AMP-dependent kinas. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3004–3008. doi: 10.1073/pnas.72.8.3004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Laloux M., Hers H. G. Native and latent forms of skeletal muscle phosphorylase phosphatase. FEBS Lett. 1979 Sep 15;105(2):239–243. doi: 10.1016/0014-5793(79)80620-1. [DOI] [PubMed] [Google Scholar]
  12. Miller T. B., Exton J. H., Park C. R. A block in epinephrine-induced glycogenolysis in hearts from adrenalectomized rats. J Biol Chem. 1971 Jun 10;246(11):3672–3678. [PubMed] [Google Scholar]
  13. Nimmo G. A., Cohen P. The regulation of glycogen metabolism. Phosphorylation of inhibitor-1 from rabbit skeletal muscle, and its interaction with protein phosphatases-III and -II. Eur J Biochem. 1978 Jun 15;87(2):353–365. doi: 10.1111/j.1432-1033.1978.tb12384.x. [DOI] [PubMed] [Google Scholar]
  14. Nimmo G. A., Cohen P. The regulation of glycogen metabolism. Purification and characterisation of protein phosphatase inhibitor-1 from rabbit skeletal muscle. Eur J Biochem. 1978 Jun 15;87(2):341–351. doi: 10.1111/j.1432-1033.1978.tb12383.x. [DOI] [PubMed] [Google Scholar]
  15. Schaeffer L. D., Chenoweth M., Dunn A. Adrenal corticosteroid involvement in the control of liver glycogen phosphorylase activity. Biochim Biophys Acta. 1969 Nov 18;192(2):292–303. doi: 10.1016/0304-4165(69)90368-7. [DOI] [PubMed] [Google Scholar]
  16. Schaeffer L. D., Chenoweth M., Dunn A. Adrenal corticosteroid involvement in the control of phosphorylase in muscle. Biochim Biophys Acta. 1969 Nov 18;192(2):304–309. doi: 10.1016/0304-4165(69)90369-9. [DOI] [PubMed] [Google Scholar]
  17. Tao S. H., Huang F. L., Lynch A., Glinsmann W. H. Control of rat skeletal-muscle phosphorylase phosphatase activity by adrenaline. Biochem J. 1978 Oct 15;176(1):347–350. doi: 10.1042/bj1760347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WOLLENBERGER A., RISTAU O., SCHOFFA G. [A simple technic for extremely rapid freezing of large pieces of tissue]. Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:399–412. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES