Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Oct;77(10):5769–5773. doi: 10.1073/pnas.77.10.5769

Variations in properties of virus released from morphologically different cell lines transformed in vitro by Friend leukemia virus.

D Tsuei, B G Pogo, C Friend
PMCID: PMC350152  PMID: 6160580

Abstract

The properties of the virus synthesized by each of three morphologically different cell lines originating from DBA/2J fetal liver cells transformed by the anemic strain of Friend leukemia virus in vitro were analyzed. The cells of line G-1 are malignant in syngeneic DBA/2 mice, grow in suspension, and are erythroid in origin. Cells of lines G-2 and G-3 are adherent, are epithelial in appearance, and produce no tumors in DBA/2J mice. Higher reverse transcriptase activity was detected in the culture fluid of lines G-2 and G-3, although virus from G-1 cells was more leukemogenic. Differences were also found in the virion density and size of the viral genome. RNA from the virions produced by G-2 and G-3 cells sedimented at 75 S in a sucrose gradient; virion RNA from G-1 cells sedmiented at 60 S. However, when subjected to polyacrylamide gel electrophoresis, all three virus strains showed identical RNA subunits with an estimated molecular weight of 2.6 X 10(6). Analysis of virion proteins by slab gel electrophoresis showed differences in envelope protein (gp71) components but not in the major core protein (p30). The properties of these viruses are stable and remain unchanged after passage in 3T3 cells.

Full text

PDF
5769

Images in this article

5772Image
on p.5772

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berkower I., Leis J., Hurwitz J. Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid in ribonucleic acid-deoxyribonucleic acid hybrid structures. J Biol Chem. 1973 Sep 10;248(17):5914–5921. [PubMed] [Google Scholar]
  2. Bolognesi D. P., Montelaro R. C., Frank H., Schäfer W. Assembly of type C oncornaviruses: a model. Science. 1978 Jan 13;199(4325):183–186. doi: 10.1126/science.202022. [DOI] [PubMed] [Google Scholar]
  3. Cheung K. S., Smith R. E., Stone M. P., Joklik W. K. Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles. Virology. 1972 Dec;50(3):851–864. doi: 10.1016/0042-6822(72)90439-4. [DOI] [PubMed] [Google Scholar]
  4. Dube S., Kung H. J., Bender W., Davidson N., Ostertag W. Size, subunit composition, and secondary structure of the Friend virus genome. J Virol. 1976 Oct;20(1):264–272. doi: 10.1128/jvi.20.1.264-272.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eckner R. J., Hettrick K. L. Defective Friend spleen focus-forming virus: interfering properties and isolation free from standard leukemia-inducing helper virus. J Virol. 1977 Oct;24(1):383–396. doi: 10.1128/jvi.24.1.383-396.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Golde D. W., Bersch N., Friend C., Tsuei D., Marovitz W. Transformation of DBA/2 mouse fetal liver cells infected in vitro by the anemic strain of Friend leukemia virus. Proc Natl Acad Sci U S A. 1979 Feb;76(2):962–966. doi: 10.1073/pnas.76.2.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Golde D. W., Faille A., Sullivan A., Friend C. Granulocytic stem cells in Friend leukemia. Cancer Res. 1976 Jan;36(1):115–119. [PubMed] [Google Scholar]
  8. Green M., Rokutanda M., Fujinaga K., Ray R. K., Rokutanda H., Gurgo C. Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses. Proc Natl Acad Sci U S A. 1970 Sep;67(1):385–393. doi: 10.1073/pnas.67.1.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. King A. M. High molecular weight RNAs from Rous sarcoma virus and Moloney murine leukemia virus contain two subunits. J Biol Chem. 1976 Jan 10;251(1):141–149. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Maisel J., Klement V., Lai M. M., Ostertag W., Duesberg P. Ribonucleic acid components of murine sarcoma and leukemia viruses. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3536–3540. doi: 10.1073/pnas.70.12.3536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oliff A. I., Hager G. L., Chang E. H., Scolnick E. M., Chan H. W., Lowy D. R. Transfection of molecularly cloned Friend murine leukemia virus DNA yields a highly leukemogenic helper-independent type C virus. J Virol. 1980 Jan;33(1):475–486. doi: 10.1128/jvi.33.1.475-486.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ostertag W., Melderis H., Steinheider G., Kluge N., Dube S. Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nat New Biol. 1972 Oct 25;239(95):231–234. doi: 10.1038/newbio239231a0. [DOI] [PubMed] [Google Scholar]
  14. Ostertag W., Pragnell I. B. Changes in genome composition of the Friend virus complex in erythroleukemia cells during the course of differentiation induced by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3278–3282. doi: 10.1073/pnas.75.7.3278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Robertson H. D., Webster R. E., Zinder N. D. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82–91. [PubMed] [Google Scholar]
  16. Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
  17. Scher W., Friend C. Breakage of DNA and alterations in folded genomes by inducers of differentiation in Friend erythroleukemic cells. Cancer Res. 1978 Mar;38(3):841–849. [PubMed] [Google Scholar]
  18. Scher W., Tsuei D., Sassa S., Price P., Gabelman N., Friend C. Inhibition of dimethyl sulfoxide-stimulated Friend cell erythrodifferentiation by hydrocortisone and other steroids. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3851–3855. doi: 10.1073/pnas.75.8.3851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schmukler M., Jewett P. B., Levy C. C. The effects of polyamines on a residue-specific human plasma ribonuclease. J Biol Chem. 1975 Mar 25;250(6):2206–2212. [PubMed] [Google Scholar]
  20. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  21. Steeves R. A., Eckner R. J., Bennett M., Mirand E. A., Trudel P. J. Isolation and characterization of a lymphatic leukemia virus in the Friend virus complex. J Natl Cancer Inst. 1971 Jun;46(6):1209–1217. [PubMed] [Google Scholar]
  22. Tambourin P. E., Wendling F. Target cell for oncogenic action of polycythaemia-inducing Friend virus. Nature. 1975 Jul 24;256(5515):320–322. doi: 10.1038/256320a0. [DOI] [PubMed] [Google Scholar]
  23. Toyoshima K., Vogt P. K. Enhancement and inhibition of avian sarcoma viruses by polycations and polyanions. Virology. 1969 Jul;38(3):414–426. doi: 10.1016/0042-6822(69)90154-8. [DOI] [PubMed] [Google Scholar]
  24. Tsuei D., Haubenstock H., Revoltella R., Friend C. Virus production and hemoglobin synthesis in variant lines of dimethyl sulfoxide-treated Friend erythroleukemia cells. J Virol. 1979 Jul;31(1):178–183. doi: 10.1128/jvi.31.1.178-183.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES