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. 1980 Oct;77(10):5789–5793. doi: 10.1073/pnas.77.10.5789

Untranslated mRNA for a chorion protein of Drosophila melanogaster accumulates transiently at the onset of specific gene amplification.

G Thireos, R Griffin-Shea, F C Kafatos
PMCID: PMC350156  PMID: 6777775

Abstract

In the cytoplasm of follicular cells of female fruitflies, a messenger RNA for one of the chorion proteins accumulates prematurely (i.e, before in vivo synthesis of the protein) but is not associated with polysomes. Subsequently, this mRNA is rapidly degraded and, thus, is not stored for later use. At a later stage in choriogenesis, the same mRNA reappears, accumulates, associates with polysomes, and is translated into a chorion protein. The transient, premature accumulation of the mRNA occurs in concert with the onset of amplification of its specific gene, a finding suggestive of a functional coupling between the two events.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton T. H., Lodish H. F. Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Dev Biol. 1977 Oct 1;60(1):180–206. doi: 10.1016/0012-1606(77)90118-x. [DOI] [PubMed] [Google Scholar]
  2. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Artavanis-Tsakonas S., Schedl P., Tschudi C., Pirrotta V., Steward R., Gehring W. J. The 5S genes of Drosophila melanogaster. Cell. 1977 Dec;12(4):1057–1067. doi: 10.1016/0092-8674(77)90169-6. [DOI] [PubMed] [Google Scholar]
  4. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  5. Beadle G. W., Ephrussi B. The Differentiation of Eye Pigments in Drosophila as Studied by Transplantation. Genetics. 1936 May;21(3):225–247. doi: 10.1093/genetics/21.3.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Griffin-Shea R., Thireos G., Kafatos F. C., Petri W. H., Villa-Komaroff L. Chorion cDNA clones of D. melanogaster and their use in studies of sequence homology and chromosomal location of chorion genes. Cell. 1980 Apr;19(4):915–922. doi: 10.1016/0092-8674(80)90083-5. [DOI] [PubMed] [Google Scholar]
  7. Lindquist S. Varying patterns of protein synthesis in Drosophila during heat shock: implications for regulation. Dev Biol. 1980 Jun 15;77(2):463–479. doi: 10.1016/0012-1606(80)90488-1. [DOI] [PubMed] [Google Scholar]
  8. Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
  9. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Palmiter R. D., Moore P. B., Mulvihill E. R. A significant lag in the induction of ovalbumin messenger RNA by steroid hormones: a receptor translocation hypothesis. Cell. 1976 Aug;8(4):557–572. doi: 10.1016/0092-8674(76)90224-5. [DOI] [PubMed] [Google Scholar]
  11. Petri W. H., Wyman A. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. III. The eggshell proteins of Drosophila melanogaster and their program of synthesis. Dev Biol. 1976 Mar;49(1):185–199. doi: 10.1016/0012-1606(76)90266-9. [DOI] [PubMed] [Google Scholar]
  12. Robb J. A. Maintenance of imaginal discs of Drosophila melanogaster in chemically defined media. J Cell Biol. 1969 Jun;41(3):876–885. doi: 10.1083/jcb.41.3.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Roop D. R., Nordstrom J. L., Tsai S. Y., Tsai M. J., O'Malley B. W. Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors. Cell. 1978 Oct;15(2):671–685. doi: 10.1016/0092-8674(78)90035-1. [DOI] [PubMed] [Google Scholar]
  14. Spradling A. C., Digan M. E., Mahowald A. P., Scott M., Craig E. A. Two clusters of genes for major chorion proteins of Drosophila melanogaster. Cell. 1980 Apr;19(4):905–914. doi: 10.1016/0092-8674(80)90082-3. [DOI] [PubMed] [Google Scholar]
  15. Spradling A. C., Mahowald A. P. Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096–1100. doi: 10.1073/pnas.77.2.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Suzuki Y., Suzuki E. Quantitative measurements of fibroin messenger RNA synthesis in the posterior silk gland of normal and mutant Bombyx mori. J Mol Biol. 1974 Sep 15;88(2):393–407. doi: 10.1016/0022-2836(74)90490-2. [DOI] [PubMed] [Google Scholar]
  17. Thireos G., Kafatos F. C. Cell-free translation of silkmoth chorion mRNAs: identification of protein precursors and characterization of cloned DNAs by hybrid-selected translation. Dev Biol. 1980 Jul;78(1):36–46. doi: 10.1016/0012-1606(80)90316-4. [DOI] [PubMed] [Google Scholar]
  18. Thireos G., Shea R. G., Kafatos F. C. Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6279–6283. doi: 10.1073/pnas.76.12.6279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Waring G. L., Mahowald A. P. Identification and time of synthesis of chorion proteins in Drosophila melanogaster. Cell. 1979 Mar;16(3):599–607. doi: 10.1016/0092-8674(79)90033-3. [DOI] [PubMed] [Google Scholar]

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