Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Oct;77(10):5972–5974. doi: 10.1073/pnas.77.10.5972

Human leukocyte interferon: structural and biological relatedness to adrenocorticotropic hormone and endorphins.

J E Blalock, E M Smith
PMCID: PMC350194  PMID: 6160589

Abstract

Anti-alpha-corticotropin [anti-ACTH alpha (1-13)](also alpha-melanotropin) and anti-gamma-endorphin antisera neutralized human leukocyte interferon activity but not fibroblast interferon activity. Human leukocyte interferon was not neutralized by anti-human lutenizing hormone (lutropin) or follicle-stimulating hormone (follitropin) antisra. Conversely, antisera to human leukocyte interferon neutralized ACTH activity. The neturalization of human leukocyte interferon by anti-human leukocyte interferon serum was partially blocked by ACTH. These studies show strong antigenic relatedness among human leukocyte interferon, ACTH, and endorphins, implying that there are underlying structural similarities. Structural relatedness is shown by pepsin cleavage of ACTH activity from human leukocyte interferon. The implication for the natural functions of human leukocyte interferon are discussed.

Full text

PDF
5972

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUONASSISI V., SATO G., COHEN A. I. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1184–1190. doi: 10.1073/pnas.48.7.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blalock J. E., Georgiades J. A., Langford M. P., Johnson H. M. Purified human immune interferon has more potent anticellular activity than fibroblast or leukocyte interferon. Cell Immunol. 1980 Feb;49(2):390–394. doi: 10.1016/0008-8749(80)90041-6. [DOI] [PubMed] [Google Scholar]
  3. Blalock J. E., Langford M. P., Georgiades J., Stanton G. J. Nonsensitized lymphocytes produce leukocyte interferon when cultured with foreign cells. Cell Immunol. 1979 Mar 1;43(1):197–201. doi: 10.1016/0008-8749(79)90163-1. [DOI] [PubMed] [Google Scholar]
  4. Blalock J. E., Stanton J. D. Common pathways of interferon and hormonal action. Nature. 1980 Jan 24;283(5745):406–408. doi: 10.1038/283406a0. [DOI] [PubMed] [Google Scholar]
  5. Campbell J. B., Grunberger T., Kochman M. A., White S. L. A microplaque reduction assay for human and mouse interferon. Can J Microbiol. 1975 Aug;21(8):1247–1253. doi: 10.1139/m75-186. [DOI] [PubMed] [Google Scholar]
  6. Chany C., Rousset S., Bourgeade M. F., Mathieu D., Grégoire A. Role of receptors and the cytoskeleton in reverse transformation and steroidogenesis induced by interferon. Ann N Y Acad Sci. 1980;350:254–265. doi: 10.1111/j.1749-6632.1980.tb20626.x. [DOI] [PubMed] [Google Scholar]
  7. Donta S. T., Smith D. M. Stimulation of steroidogenesis in tissue culture by enterotoxigenic Escherichia coli and its neutralization by specific antiserum. Infect Immun. 1974 Mar;9(3):500–505. doi: 10.1128/iai.9.3.500-505.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gresser I., Tovey M. G. Antitumor effects of interferon. Biochim Biophys Acta. 1978 Oct 27;516(2):231–247. doi: 10.1016/0304-419x(78)90009-4. [DOI] [PubMed] [Google Scholar]
  9. ISAACS A., LINDENMANN J. Virus interference. I. The interferon. Proc R Soc Lond B Biol Sci. 1957 Sep 12;147(927):258–267. doi: 10.1098/rspb.1957.0048. [DOI] [PubMed] [Google Scholar]
  10. Knight E., Jr, Hunkapiller M. W., Korant B. D., Hardy R. W., Hood L. E. Human fibroblast interferon: amino acid analysis and amino terminal amino acid sequence. Science. 1980 Feb 1;207(4430):525–526. doi: 10.1126/science.7352259. [DOI] [PubMed] [Google Scholar]
  11. Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  13. Zoon K. C., Smith M. E., Bridgen P. J., Anfinsen C. B., Hunkapiller M. W., Hood L. E. Amino terminal sequence of the major component of human lymphoblastoid interferon. Science. 1980 Feb 1;207(4430):527–528. doi: 10.1126/science.7352260. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES