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. 1980 Nov;77(11):6334–6338. doi: 10.1073/pnas.77.11.6334

Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase.

T Y Li, A J Kraker, C F Shaw 3rd, D H Petering
PMCID: PMC350278  PMID: 6779278

Abstract

The reactions of Zn-, Zn,Cd-, and Cd-thioneins with EDTA and apo-carbonic anhydrase have been studied. The ligand substitution reaction of zinc with EDTA is multiphasic, having both associative and dissociative components in the rate expression. The cadmium sites are about 2 orders of magnitude less reactive. In contrast, apo-carbonic anhydrase abstracts zinc from Zn-thionein and Zn,Cd-thionein in second-order processes that are 2-3 orders of magnitude more rapid than those involving EDTA and approach the rate for unligated Zn2+ with the apo-protein. In comparison with other zinc proteins, Zn-thionein contains unusually reactive metal sites, suggesting that this protein may be a physiological zinc transfer protein, able to donate zinc to zinc-requiring apo macromolecules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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