Abstract
The synthesis and evaluation of a new detergent that is a zwitterionic derivative of cholic acid is presented. This detergent combines the useful properties of both the sulfobetaine-type detergents and the bile salt anions. The new detergent proved to be effective at solubilizing membrane proteins in a nondenatured state.
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Selected References
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- Coleman R., Holdsworth G., Finean J. B. Detergent extraction of erythrocyte ghosts. Comparison of residues after cholate and Triton X-100 treatments. Biochim Biophys Acta. 1976 Jun 4;436(1):38–44. doi: 10.1016/0005-2736(76)90217-0. [DOI] [PubMed] [Google Scholar]
- Gonenne A., Ernst R. Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants. Anal Biochem. 1978 Jun 15;87(1):28–38. doi: 10.1016/0003-2697(78)90565-1. [DOI] [PubMed] [Google Scholar]
- Helenius A., McCaslin D. R., Fries E., Tanford C. Properties of detergents. Methods Enzymol. 1979;56:734–749. doi: 10.1016/0076-6879(79)56066-2. [DOI] [PubMed] [Google Scholar]
- Helenius A., Simons K. Solubilization of membranes by detergents. Biochim Biophys Acta. 1975 Mar 25;415(1):29–79. doi: 10.1016/0304-4157(75)90016-7. [DOI] [PubMed] [Google Scholar]
- Hjelmeland L. M., Nebert D. W., Chrambach A. Electrofocusing of integral membrane proteins in mixtures of zwitterionic and nonionic detergents. Anal Biochem. 1979 May;95(1):201–208. doi: 10.1016/0003-2697(79)90206-9. [DOI] [PubMed] [Google Scholar]
- Simonds W. F., Koski G., Streaty R. A., Hjelmeland L. M., Klee W. A. Solubilization of active opiate receptors. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4623–4627. doi: 10.1073/pnas.77.8.4623. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]
- Tanford C., Reynolds J. A. Characterization of membrane proteins in detergent solutions. Biochim Biophys Acta. 1976 Oct 26;457(2):133–170. doi: 10.1016/0304-4157(76)90009-5. [DOI] [PubMed] [Google Scholar]
- Vyvoda O. S., Coleman R., Holdsworth G. Effects of different bile salts upon the composition and morphology of a liver plasma membrane preparation. Deoxycholate is more membrane damaging than cholate and its conjugates. Biochim Biophys Acta. 1977 Feb 14;465(1):68–76. doi: 10.1016/0005-2736(77)90356-x. [DOI] [PubMed] [Google Scholar]