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. 2012 Oct 22;1(10):e31. doi: 10.1038/oncsis.2012.31

Figure 5.

Figure 5

WNT3/FZD7-mediated signaling promotes invasion and anchorage-independent growth. (a, b) Analysis of invasion using a matrigel-coated transwell assay in HepaRG (a) and OUMS-29 (b). Left: Representative photographs of stained cells ( × 100). Right: Quantification of invasive cells using Stereologer. (c) Results of soft agar colony formation assay with OUMS-29-derived clones. Top: Representative microscopic photographs of colony size and number ( × 40). Bottom: The number of colonies was calculated from macroscopic photographs of the wells. *P<0.01 and **P<0.05 compared with the negative controls OU-EV or RG-EV.