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. 1980 Dec;77(12):7242–7246. doi: 10.1073/pnas.77.12.7242

Vanadate inhibition of auxin-enhanced H+ secretion and elongation in pea epicotyls and oat coleoptiles

Mark Jacobs 1,*, Lincoln Taiz 1
PMCID: PMC350478  PMID: 16592936

Abstract

In experiments carried out to investigate the acid secretion theory of auxin action, we utilized sodium orthovanadate, an agent found to be a selective inhibitor of a plasma membrane-associated H+-pumping ATPase in Neurospora [Bowman, B. J. & Slayman, C. W. (1979) J. Biol. Chem. 245, 2928-2934]. At 1 mM, vanadate inhibited auxin-enhanced medium acidification by pea epicotyl segments within 5 min, whether added 0.5 or 2.5 hr after auxin. Inhibition of acidification was total after 10-15 min but could be reversed within 10 min after vanadate removal. When given as a 40-min pretreatment, vanadate completely prevented any auxin-enhanced acidification. Vanadate inhibition of medium acidification by oat coleoptile segments was also total and reversible, but both inhibition and reversal occurred after longer lag times than in pea. Inhibitory effects of vanadate on elongation in pea and oat tissue closely paralleled its effects on acidification, and the inhibitory effect of vanadate on elongation could be reversed by an acidic buffer. Vanadate did not inhibit respiration or protein synthesis in pea epicotyl segments, although it strongly inhibited L-[14C]leucine uptake. These results indicate the importance of cell wall acidification for short- and long-term auxin-enhanced growth and suggest the participation in wall acidification of a plasma membrane-associated ATPase acting as an H+ pump.

Keywords: cell wall acidification, ATPase

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman B. J., Slayman C. W. The effects of vanadate on the plasma membrane ATPase of Neurospora crassa. J Biol Chem. 1979 Apr 25;254(8):2928–2934. [PubMed] [Google Scholar]
  2. Cleland R. E. Kinetics of Hormone-induced H Excretion. Plant Physiol. 1976 Aug;58(2):210–213. doi: 10.1104/pp.58.2.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cleland R. E., Prins H. B., Harper J. R., Higinbotham N. Rapid Hormone-induced Hyperpolarization of the Oat Coleoptile Transmembrane Potential. Plant Physiol. 1977 Mar;59(3):395–397. doi: 10.1104/pp.59.3.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cleland R. Auxin-induced hydrogen ion excretion from Avena coleoptiles. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3092–3093. doi: 10.1073/pnas.70.11.3092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DeMaster E. G., Mitchell A. A comparison of arsenate and vanadate as inhibitors or uncouplers of mitochondrial and glycolytic energy metabolism. Biochemistry. 1973 Sep 11;12(19):3616–3621. doi: 10.1021/bi00743a007. [DOI] [PubMed] [Google Scholar]
  6. Etherton B. Effect of Indole-3-acetic Acid on Membrane Potentials of Oat Coleoptile Cells. Plant Physiol. 1970 Apr;45(4):527–528. doi: 10.1104/pp.45.4.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Etherton B. Evidence for amino Acid-h co-transport in oat coleoptiles. Plant Physiol. 1978 Jun;61(6):933–937. doi: 10.1104/pp.61.6.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jacobs M., Ray P. M. Promotion of Xyloglucan Metabolism by Acid pH. Plant Physiol. 1975 Sep;56(3):373–376. doi: 10.1104/pp.56.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacobs M., Ray P. M. Rapid Auxin-induced Decrease in Free Space pH and Its Relationship to Auxin-induced Growth in Maize and Pea. Plant Physiol. 1976 Aug;58(2):203–209. doi: 10.1104/pp.58.2.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Josephson L., Cantley L. C., Jr Isolation of a potent (Na-K)ATPase inhibitor from striated muscle. Biochemistry. 1977 Oct 18;16(21):4572–4578. doi: 10.1021/bi00640a006. [DOI] [PubMed] [Google Scholar]
  11. O'Neal S. G., Rhoads D. B., Racker E. Vanadate inhibition of sarcoplasmic reticulum Ca2+-ATPase and other ATPases. Biochem Biophys Res Commun. 1979 Aug 13;89(3):845–850. doi: 10.1016/0006-291x(79)91855-2. [DOI] [PubMed] [Google Scholar]
  12. Rayle D. L., Cleland R. Enhancement of wall loosening and elongation by Acid solutions. Plant Physiol. 1970 Aug;46(2):250–253. doi: 10.1104/pp.46.2.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vanderhoef L. N., Lu T. Y., Williams C. A. Comparison of Auxin-induced and Acid-induced Elongation in Soybean Hypocotyl. Plant Physiol. 1977 May;59(5):1004–1007. doi: 10.1104/pp.59.5.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]

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