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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Dec;77(12):7297–7301. doi: 10.1073/pnas.77.12.7297

Cell cycle and the differential expression of HLA-A,B and HLA-DR antigens on human B lymphoid cells.

S Sarkar, M C Glassy, S Ferrone, O W Jones
PMCID: PMC350489  PMID: 6971443

Abstract

Monoclonal antibodies specific to HLA antigens and the fluorescence-activated cell sorter were used to analyze the changes in the density of human histocompatibility antigens HLA-A,B and HLA-DR on the surface of synchronously growing WI-L2 cells (a human B cell line) progressing through the cell cycle. The WI-L2 cells were synchronized by density-dependent arrest in G1, and samples from G0, G1, late S and late G2 phases were used to determine the frequency distribution of cell volume, DNA content, and the relative amounts of cell surface HLA antigens; the observed density changes were calculated from these values. The HLA-A,B density remained nearly constant throughout the cell cycle, whereas the HLA-DR density increased sharply at the G2-M stage. These results suggest a cell cycle-dependent differential control of the expression of these two sets of histocompatibility antigens on B cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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