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. 1980 Dec;77(12):7333–7337. doi: 10.1073/pnas.77.12.7333

Replication of plasmids from Staphylococcus aureus in Escherichia coli.

A Goze, S D Ehrlich
PMCID: PMC350497  PMID: 7012836

Abstract

Plasmid pBR322 derives from plasmid ColE1 and does not replicate in Escherichia coli strains lacking DNA polymerase I. Hybrids between pBR322 and a plasmid isolated from Staphylococcus aureus, pC194, replicate in such E. coli strains, provided that the pC194 replication region is intact. Inactivation of the pBR322 replication region does not interfere with the replication of hybrids in E. coli. Hybrids between pBR322 and two other plasmids from S. aureus, pT127 and pUB112, and replicate at the restrictive temperature in E. coli having thermosensitive DNA polymerase I. Similar hybrids involving pC221 and pHV400, plasmids from S. aureus and Bacillus subtilis, respectively, do not replicate under such conditions. These results show that some plasmids from a Gram-positive bacterium, S. aureus, can replicate in a Gram-negative one, E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Backman K., Betlach M., Boyer H. W., Yanofsky S. Genetic and physical studies on the replication of ColE1-type plasmids. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):69–76. doi: 10.1101/sqb.1979.043.01.012. [DOI] [PubMed] [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  4. CHABBERT Y. A., BAUDENS J. G., GERBAUD G. R. VARIATIONS SOUS L'INFLUENCE DE L'ACRIFLAVINE ET TRANSDUCTION DE LA R'ESISTANCE A LA KANAMYCINE ET AU CHLORAMPH'ENICOL CHEZ LES STAPHYLOCOQUES. Ann Inst Pasteur (Paris) 1964 Nov;107:678–690. [PubMed] [Google Scholar]
  5. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colman A., Byers M. J., Primrose S. B., Lyons A. Rapid purification of plasmid DNAs by hydroxyapatite chromatography. Eur J Biochem. 1978 Nov 2;91(1):303–310. doi: 10.1111/j.1432-1033.1978.tb20966.x. [DOI] [PubMed] [Google Scholar]
  7. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  8. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  9. Domaradskii I. V., Levadnaia T. B., Sitnikov B. S., Rassadin A. S., Denisova T. S. Transformatsiia Bacillus subtilis izolirovannoi DNK plazmid R. Dokl Akad Nauk SSSR. 1976 Feb 21;226(6):1443–1445. [PubMed] [Google Scholar]
  10. Donoghue D. J., Sharp P. A. Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins. J Bacteriol. 1978 Mar;133(3):1287–1294. doi: 10.1128/jb.133.3.1287-1294.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ehrlich S. D., Bursztyn-Pettegrew H., Stroynowski I., Lederberg J. Expression of the thymidylate synthetase gene of the Bacillus subtilis bacteriophage Phi-3-T in Escherichia coli. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4145–4149. doi: 10.1073/pnas.73.11.4145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ehrlich S. D. DNA cloning in Bacillus subtilis. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433–1436. doi: 10.1073/pnas.75.3.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ehrlich S. D. Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1680–1682. doi: 10.1073/pnas.74.4.1680. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Harris-Warrick R. M., Elkana Y., Ehrlich S. D., Lederberg J. Electrophoretic separation of Bacillus subtilis genes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207–2211. doi: 10.1073/pnas.72.6.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Iordanescu S., Surdeanu M., Della Latta P., Novick R. Incompatibility and molecular relationships between small Staphylococcal plasmids carrying the same resistance marker. Plasmid. 1978 Sep;1(4):468–479. doi: 10.1016/0147-619x(78)90005-7. [DOI] [PubMed] [Google Scholar]
  16. Iordănescu S. Incompatibility-deficient derivatives of a small staphylococcal plasmid. Plasmid. 1979 Apr;2(2):207–215. doi: 10.1016/0147-619x(79)90039-8. [DOI] [PubMed] [Google Scholar]
  17. Iordănescu S. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids. J Bacteriol. 1975 Nov;124(2):597–601. doi: 10.1128/jb.124.2.597-601.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kahn M. L., Figurski D., Ito L., Helinski D. R. Essential regions for replication of a stringent and a relaxed plasmid in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):99–103. doi: 10.1101/sqb.1979.043.01.015. [DOI] [PubMed] [Google Scholar]
  19. Kahn M., Helinski D. R. Construction of a novel plasmid-phage hybrid: use of the hybrid to demonstrate ColE1 DNA replication in vivo in the absence of a ColE1-specified protein. Proc Natl Acad Sci U S A. 1978 May;75(5):2200–2204. doi: 10.1073/pnas.75.5.2200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  21. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973 Jun;114(3):1116–1124. doi: 10.1128/jb.114.3.1116-1124.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Naumov L. S., Numerov V. K., Bozhko A. N., Lushnikov A. A., Tiurim V. S. Geterologichnaia transformatsiia u Bac. subtilis. Soobshchenie 1. Peredacha DNK plazmidy R1drd19. Zh Mikrobiol Epidemiol Immunobiol. 1977 May;(5):33–37. [PubMed] [Google Scholar]
  23. Niaudet B., Ehrlich S. D. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400. Plasmid. 1979 Jan;2(1):48–58. doi: 10.1016/0147-619x(79)90005-2. [DOI] [PubMed] [Google Scholar]
  24. Novick R. Plasmid-protein relaxation complexes in Staphylococcus aureus. J Bacteriol. 1976 Sep;127(3):1177–1187. doi: 10.1128/jb.127.3.1177-1187.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ohmori H., Tomizawa J. Nucleotide sequence of the region required for maintenance of colicin E1 plasmid. Mol Gen Genet. 1979 Oct 3;176(2):161–170. doi: 10.1007/BF00273210. [DOI] [PubMed] [Google Scholar]
  26. Oka A., Nomura N., Morita M., Sugisaki H., Sugimoto K., Takanami M. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity. Mol Gen Genet. 1979 May 4;172(2):151–159. doi: 10.1007/BF00268276. [DOI] [PubMed] [Google Scholar]
  27. Reanney D. Extrachromosomal elements as possible agents of adaptation and development. Bacteriol Rev. 1976 Sep;40(3):552–590. doi: 10.1128/br.40.3.552-590.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Ruby C., Novick R. P. Plasmid interactions in Staphylococcus aureus: nonadditivity of compatible plasmid DNA pools. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5031–5035. doi: 10.1073/pnas.72.12.5031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Sgaramella V., Ehrlich S. D. Use of the T4 polynucleotide ligase in the joining of flush-ended DNA segments generated by restriction endonucleases. Eur J Biochem. 1978 May 16;86(2):531–537. doi: 10.1111/j.1432-1033.1978.tb12336.x. [DOI] [PubMed] [Google Scholar]
  30. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  31. Taylor D. P., Cohen S. N. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1. J Bacteriol. 1979 Jan;137(1):92–104. doi: 10.1128/jb.137.1.92-104.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Tomizawa J. I., Ohmori H., Bird R. E. Origin of replication of colicin E1 plasmid DNA. Proc Natl Acad Sci U S A. 1977 May;74(5):1865–1869. doi: 10.1073/pnas.74.5.1865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wilson C. R., Baldwin N. J. Characterization and construction of molecular cloning vehicles within Staphylococcus aureus. J Bacteriol. 1978 Oct;136(1):402–413. doi: 10.1128/jb.136.1.402-413.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Yasuda S., Hirota Y. Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458–5462. doi: 10.1073/pnas.74.12.5458. [DOI] [PMC free article] [PubMed] [Google Scholar]

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