Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1980 Dec;77(12):7479–7483. doi: 10.1073/pnas.77.12.7479

Dopamine and depolarizing agents regulate the state of phosphorylation of protein I in the mammalian superior cervical sympathetic ganglion.

E J Nestler, P Greengard
PMCID: PMC350528  PMID: 6164062

Abstract

The regulation of the state of phosphorylation of protein I, a specific neuronal protein that appears to be associated predominantly with synaptic vesicles, has been studied in intact sections of bovine superior cervical ganglion. For this purpose, a technique was developed that made possible the quantitation of the state of phosphorylation of as little as 5 fmol of protein I. Incubation of ganglion sections in the presence of dopamine, 8-bromo-cyclic AMP, or depolarizing agents (i.e., high K+ concentration or veratridine) increased the state of phosphorylation of protein I relative to that of control ganglion sections. Other results indicated that the effect of dopamine is probably mediated via the activation of a cyclic AMP-dependent protein kinase and that the effect of high K+ concentration is probably mediated via the activation of a calcium-dependent protein kinase.

Full text

PDF
7479

Images in this article

7481Image
on p.7481
7481Image
on p.7481

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom F. E., Ueda T., Battenberg E., Greengard P. Immunocytochemical localization, in synapses, of protein I, an endogenous substrate for protein kinases in mammalian brain. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5982–5986. doi: 10.1073/pnas.76.11.5982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De Camilli P., Ueda T., Bloom F. E., Battenberg E., Greengard P. Widespread distribution of protein I in the central and peripheral nervous systems. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5977–5981. doi: 10.1073/pnas.76.11.5977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dun N., Nishi S. Effects of dopamine on the superior cervical ganglion of the rabbit. J Physiol. 1974 May;239(1):155–164. doi: 10.1113/jphysiol.1974.sp010560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eränkö O. Small intensely fluorescent (SIF) cells and nervous transmission in sympathetic ganglia. Annu Rev Pharmacol Toxicol. 1978;18:417–430. doi: 10.1146/annurev.pa.18.040178.002221. [DOI] [PubMed] [Google Scholar]
  5. Forn J., Greengard P. Depolarizing agents and cyclic nucleotides regulate the phosphorylation of specific neuronal proteins in rat cerebral cortex slices. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5195–5199. doi: 10.1073/pnas.75.10.5195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Greengard P., Kebabian J. W. Role of cyclic AMP in synaptic transmission in the mammalian peripheral nervous system. Fed Proc. 1974 Apr;33(4):1059–1067. [PubMed] [Google Scholar]
  7. Greengard P. Phosphorylated proteins as physiological effectors. Science. 1978 Jan 13;199(4325):146–152. doi: 10.1126/science.22932. [DOI] [PubMed] [Google Scholar]
  8. Huttner W. B., Greengard P. Multiple phosphorylation sites in protein I and their differential regulation by cyclic AMP and calcium. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5402–5406. doi: 10.1073/pnas.76.10.5402. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ivarie R. D., Jones P. P. A rapid sensitive assay for specific protein synthesis in cells and in cell-free translations: use of Staphylococcus aureus as an adsorbent for immune complexes. Anal Biochem. 1979 Aug;97(1):24–35. doi: 10.1016/0003-2697(79)90322-1. [DOI] [PubMed] [Google Scholar]
  10. Kebabian J. W., Steiner A. L., Greengard P. Muscarinic cholinergic regulation of cyclic guanosine 3,5-monophosphate in autonomic ganglia: possible role in synaptic transmission. J Pharmacol Exp Ther. 1975 May;193(2):474–488. [PubMed] [Google Scholar]
  11. Krueger B. K., Forn J., Greengard P. Depolarization-induced phosphorylation of specific proteins, mediated by calcium ion influx, in rat brain synaptosomes. J Biol Chem. 1977 Apr 25;252(8):2764–2773. [PubMed] [Google Scholar]
  12. Libet B. Generation of slow inhibitory and excitatory postsynaptic potentials. Fed Proc. 1970 Nov-Dec;29(6):1945–1956. [PubMed] [Google Scholar]
  13. Miyamota M. D., Breckenridge B. M. A cyclic adenosine monophosphate link in the catecholamine enhancement of transmitter release at the neuromuscular junction. J Gen Physiol. 1974 May;63(5):609–624. doi: 10.1085/jgp.63.5.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rosenthal J. Post-tetanic potentiation at the neuromuscular junction of the frog. J Physiol. 1969 Jul;203(1):121–133. doi: 10.1113/jphysiol.1969.sp008854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sieghart W., Forn J., Greengard P. Ca2+ and cyclic AMP regulate phosphorylation of same two membrane-associated proteins specific to nerve tissue. Proc Natl Acad Sci U S A. 1979 May;76(5):2475–2479. doi: 10.1073/pnas.76.5.2475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ueda T., Greengard P. Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. I. Solubilization, purification, and some properties of an endogenous phosphoprotein. J Biol Chem. 1977 Jul 25;252(14):5155–5163. [PubMed] [Google Scholar]
  17. Ueda T., Greengard P., Berzins K., Cohen R. S., Blomberg F., Grab D. J., Siekevitz P. Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase. J Cell Biol. 1979 Nov;83(2 Pt 1):308–319. doi: 10.1083/jcb.83.2.308. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES