Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Apr;32(1):98–104. doi: 10.1128/iai.32.1.98-104.1981

Characterization of a bactericidal lipid developing within staphylococcal abscesses.

E S Dye, F A Kapral
PMCID: PMC350593  PMID: 7216498

Abstract

Extraction of staphylococcal abscesses by the Folch procedure revealed that all of the staphylocidal activity was present in the lipid fraction. Further separation of the lipids indicated that the bactericidal activity resided in the free fatty acid pool. Lipids similarly extracted from mesenteric or epididymal fat tissue, either before of after activation, did not possess comparable activity. Myristic, palmitic, palmitoleic, linoleic, and oleic acids, as well as lysolecithin, also failed to exhibit the properties of the fatty acid fraction obtained from abscess homogenates. These findings suggest the staphylocidal fatty acid is not a common host lipid.

Full text

PDF
98

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Butcher G. W., King G., Dyke K. G. Sensitivity of Staphylococcus aureus to unsaturated fatty acids. J Gen Microbiol. 1976 Jun;94(2):290–296. doi: 10.1099/00221287-94-2-290. [DOI] [PubMed] [Google Scholar]
  3. DOLE V. P., MEINERTZ H. Microdetermination of long-chain fatty acids in plasma and tissues. J Biol Chem. 1960 Sep;235:2595–2599. [PubMed] [Google Scholar]
  4. Dye E. S., Kapral F. A. Partial characterization of a bactericidal system in staphylococcal abscesses. Infect Immun. 1980 Oct;30(1):198–203. doi: 10.1128/iai.30.1.198-203.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EISLER D. M., VONMETZ E. ANTI-PASTEURELLA PESTIS FACTOR IN THE ORGANS OF NORMAL MICE AND GUINEA PIGS. I. BIOLOGIC CHARACTERISTICS. J Immunol. 1963 Aug;91:287–294. [PubMed] [Google Scholar]
  6. Eisler D. M., Hill B., Von Metz E. K., Chang W. J., Heckly R. J. Anti-Pasteurella pestis factor from mice and guinea pigs. II. Some chemical and physical characteristics. J Immunol. 1967 Mar;98(3):576–583. [PubMed] [Google Scholar]
  7. Eisler D. M., Von Metz E. K. Anti-Pasteurella pestis factor. 3. Effects of fatty acids on Pasteurella pestis. J Bacteriol. 1968 May;95(5):1767–1773. doi: 10.1128/jb.95.5.1767-1773.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Galbraith H., Miller T. B. Effect of long chain fatty acids on bacterial respiration and amino acid uptake. J Appl Bacteriol. 1973 Dec;36(4):659–675. doi: 10.1111/j.1365-2672.1973.tb04151.x. [DOI] [PubMed] [Google Scholar]
  9. Galbraith H., Miller T. B. Effect of metal cations and pH on the antibacterial activity and uptake of long chain fatty acids. J Appl Bacteriol. 1973 Dec;36(4):635–646. doi: 10.1111/j.1365-2672.1973.tb04149.x. [DOI] [PubMed] [Google Scholar]
  10. Galbraith H., Miller T. B. Physicochemical effects of long chain fatty acids on bacterial cells and their protoplasts. J Appl Bacteriol. 1973 Dec;36(4):647–658. doi: 10.1111/j.1365-2672.1973.tb04150.x. [DOI] [PubMed] [Google Scholar]
  11. Gutteridge J. M., Lamport P., Dormandy T. L. Autoxidation as a cause of antibacterial activity in unsaturated fatty acids. J Med Microbiol. 1974 Aug;7(3):387–389. doi: 10.1099/00222615-7-3-387. [DOI] [PubMed] [Google Scholar]
  12. Kabara J. J., Vrable R. Antimicrobial lipids: natural and synthetic fatty acids and monoglycerides. Lipids. 1977 Sep;12(9):753–759. doi: 10.1007/BF02570908. [DOI] [PubMed] [Google Scholar]
  13. Kapral F. A., Godwin J. R., Dye E. S. Formation of intraperitoneal abscesses by Staphylococcus aureus. Infect Immun. 1980 Oct;30(1):204–211. doi: 10.1128/iai.30.1.204-211.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kochan I., Golden C. A. Antimycobacterial effect of lysates prepared from immunologically activated macrophages. Infect Immun. 1973 Sep;8(3):388–394. doi: 10.1128/iai.8.3.388-394.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kochan I., Golden C. A. Immunological nature of antimycobacterial phenomenon in macrophages. Infect Immun. 1974 Feb;9(2):249–254. doi: 10.1128/iai.9.2.249-254.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kochan I., Pellis N. R., Pfohl D. G. Effects of normal and activated cell fractions on the growth of tubercle bacilli. Infect Immun. 1972 Aug;6(2):142–148. doi: 10.1128/iai.6.2.142-148.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Miller R. D., Brown K. E., Morse S. A. Inhibitory action of fatty acids on the growth of Neisseria gonorrhoeae. Infect Immun. 1977 Aug;17(2):303–312. doi: 10.1128/iai.17.2.303-312.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sheu C. W., Freese E. Lipopolysaccharide layer protection of gram-negative bacteria against inhibition by long-chain fatty acids. J Bacteriol. 1973 Sep;115(3):869–875. doi: 10.1128/jb.115.3.869-875.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stossel T. P., Mason R. J., Smith A. L. Lipid peroxidation by human blood phagocytes. J Clin Invest. 1974 Sep;54(3):638–645. doi: 10.1172/JCI107801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Strauss R. R., Paul B. B., Jacobs A. A., Sbarra A. J. Role of the Phagocyte in Host-Parasite Interactions XXVII. Myeloperoxidase-H(2)O(2)-Cl-Mediated Aldehyde Formation and Its Relationship to Antimicrobial Activity. Infect Immun. 1971 Apr;3(4):595–602. doi: 10.1128/iai.3.4.595-602.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES