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. 1981 Aug;33(2):625–628. doi: 10.1128/iai.33.2.625-628.1981

Relationship between plasmid content and auxotype in Neisseria gonorrhoeae isolates.

J R Dillon, M Pauzé
PMCID: PMC350746  PMID: 6792083

Abstract

One hundred and forty strains of Neisseria gonorrhoeae, representing 12 different auxotype groups, were examined for differences in plasmid content. Most auxotype groups harbored a phenotypically cryptic 2,6-megadalton plasmid; a few groups also carried a 24.5-megadalton plasmid which has been previously characterized as a transfer plasmid. However, isolates of the proline-, citrulline-, and uracil-requiring (PCU-) auxotype were consistently free of plasmids. The correlation between auxotype and plasmid content is especially significant since, in Canada, PCU- isolates have the second highest prevalence of all auxotypes.

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Selected References

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  1. Davies J. K., Normark S. A relationship between plasmid structure, structural lability, and sensitivity to site-specific endonucleases in Neisseria gonorrhoeae. Mol Gen Genet. 1980 Jan;177(2):251–260. doi: 10.1007/BF00267436. [DOI] [PubMed] [Google Scholar]
  2. Dillon J. R., Duck P., Thomas D. Y. Molecular and phenotypic characterization of penicillinase-producing Neisseria gonorrhoeae from Canadian sources. Antimicrob Agents Chemother. 1981 Jun;19(6):952–957. doi: 10.1128/aac.19.6.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Engelkirk P. G., Schoenhard D. E. Physical evidence of a plasmid in Neisseria gonorrhoeae. J Infect Dis. 1973 Feb;127(2):197–200. doi: 10.1093/infdis/127.2.197. [DOI] [PubMed] [Google Scholar]
  4. Fitzgerald T. J., Miller J. N., Sykes J. A. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975 May;11(5):1141–1146. doi: 10.1128/iai.11.5.1141-1146.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Foster R. S., Foster G. C. Electrophoretic comparison of endonuclease-digested plasmids from Neisseria gonorrhoeae. J Bacteriol. 1976 Jun;126(3):1297–1304. doi: 10.1128/jb.126.3.1297-1304.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hendry A. T., Stewart I. O. Auxanographic grouping and typing of Neisseria gonorrhoeae. Can J Microbiol. 1979 Apr;25(4):512–521. doi: 10.1139/m79-075. [DOI] [PubMed] [Google Scholar]
  7. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mayer L. W., Holmes K. K., Falkow S. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae. Infect Immun. 1974 Oct;10(4):712–717. doi: 10.1128/iai.10.4.712-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Norlander L., Davies J., Normark S. Genetic exchange mechanisms in Neisseria gonorrhoeae. J Bacteriol. 1979 Jun;138(3):756–761. doi: 10.1128/jb.138.3.756-761.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roberts M., Falkow S. Conjugal transfer of R plasmids in Neisseria gonorrhoeae. Nature. 1977 Apr 14;266(5603):630–631. doi: 10.1038/266630a0. [DOI] [PubMed] [Google Scholar]
  11. Roberts M., Piot P., Falkow S. The ecology of gonococcal plasmids. J Gen Microbiol. 1979 Oct;114(2):491–494. doi: 10.1099/00221287-114-2-491. [DOI] [PubMed] [Google Scholar]
  12. Shepard H. M., Polisky B. Measurement of Plasmid copy number. Methods Enzymol. 1979;68:503–513. doi: 10.1016/0076-6879(79)68039-4. [DOI] [PubMed] [Google Scholar]
  13. Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol. 1979;68:152–176. doi: 10.1016/0076-6879(79)68011-4. [DOI] [PubMed] [Google Scholar]
  14. Sox T. E., Mohammed W., Blackman E., Biswas G., Sparling P. F. Conjugative plasmids in Neisseria gonorrhoeae. J Bacteriol. 1978 Apr;134(1):278–286. doi: 10.1128/jb.134.1.278-286.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stiffler P. W., Lerner S. A., Bohnhoff M., Morello J. A. Plasmid deoxyribonucleic acid in clinical isolates of Neisseria gonorrhoeae. J Bacteriol. 1975 Jun;122(3):1293–1300. doi: 10.1128/jb.122.3.1293-1300.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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