Table 2.

Dosage and immunization schedule of female BALB/c mice immunized with bivalent formulation of HPV16 and HPV18 VLPs expressed in Pichia pastoris. Last column shows the geometric mean titers of 35th and 190th day serum antibody in immunized mice. Range of antibody titers in each group is mentioned in parenthesis.

Group	Treatment	Dosage (μg)	No of mice	Immunization days	Bleeding days	GMT Serum antibody (range)
						Against 16 VLP		Against 18 VLP
						35th day	190th day	35th day	190th day
1	Bivalent (HPV16 and 18 VLP) vaccine	HPV16 VLP – 2 μg and HPV18 VLP – 1 μg	7	0, 14 and 28	0, 14, 28, 35 and 190	5796 (800–25,600)	317 (100–800)	2625 (1600–25,600)	400 (100–1600)
2	Placebo	Aluminium Hydroxide	7	0, 14 and 28	0, 14, 28, 35 and 190	0	0	0	0