Abstract
Strain CM5 of Haemophilus pleuropneumoniae was toxic for porcine lung macrophages in minimum doses of 10(6) colony-forming units per ml and for peripheral blood monocytes at 10(7) colony-forming units per ml. The organism was not toxic for porcine testicular cells in a concentration of 10(8) colony-forming units per ml. Filtered sterile culture supernatant diluted 10(0), 10(-1), and 10(-2) was toxic for porcine pulmonary lavage cells. In dilutions of 10(0) and 10(-1), culture supernatant was toxic for peripheral blood monocytes, and at 10(0) it was toxic for testicular cells. Toxicity associated with bacterial cells was sensitive to heating (60 degrees, 60 min), whereas that of the culture supernatant was heat stable (100 degrees C, 15 min). Swine convalescent serum neutralized supernatant toxicity in dilutions of greater than or equal to 1:320. The findings promote the understanding of the pathogenesis of pleuropneumonia in swine caused by H. pleuropneumoniae.
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- Davis W. B., Barsoum I. S., Ramwell P. W., Yeager H., Jr Human alveolar macrophages: effects of endotoxin in vitro. Infect Immun. 1980 Dec;30(3):753–758. doi: 10.1128/iai.30.3.753-758.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Markham R. J., Wilkie B. N. Interaction between Pasteurella haemolytica and bovine alveolar macrophages: cytotoxic effect on macrophages and impaired phagocytosis. Am J Vet Res. 1980 Jan;41(1):18–22. [PubMed] [Google Scholar]
- SHOPE R. E. PORCINE CONTAGIOUS PLEUROPNEUMONIA. I. EXPERIMENTAL TRANSMISSION, ETIOLOGY, AND PATHOLOGY. J Exp Med. 1964 Mar 1;119:357–368. doi: 10.1084/jem.119.3.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilkie B. N., Caoili F., Jacobs R. Bovine lymphocytes: erythrocyte rosettes in normal, lymphomatous and corticosteroid-treated cattle. Can J Comp Med. 1979 Jan;43(1):22–28. [PMC free article] [PubMed] [Google Scholar]