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Infection and Immunity logoLink to Infection and Immunity
. 1981 Sep;33(3):775–778. doi: 10.1128/iai.33.3.775-778.1981

Increased content of microbicidal cationic peptides in rabbit alveolar macrophages elicited by complete Freund adjuvant.

R I Lehrer, D Szklarek, M E Selsted, J Fleischmann
PMCID: PMC350777  PMID: 7287180

Abstract

We measured the microbicidal peptides MCP-1 and MCP-2 in rabbit alveolar macrophages (AM), comparing rabbits pretreated with complete Freund adjuvant with untreated control animals. Levels of MCP-1 increased from 4.7 +/- 0.6 microgram/10(7) resident AM to 13.2 +/- 0.1 microgram/10(7) complete Freund adjuvant-elicited AM. MCP-2 levels rose from 1.8 +/- 0.1 microgram/10(7) resident AM to 7.3 +/- 0.4 microgram/10(7) complete Freund adjuvant-elicited AM. The activities of five lysosomal hydrolases (beta-D-glucuronidase, beta-D-galactosidase, acid phosphatase, N-acetyl-beta-D-galactosaminidase, and N-acetyl-beta-D-glucosaminidase) were 44 to 96% higher in complete Freund adjuvant-elicited AM, and lysozyme activity was three- to fourfold higher. As MCP-1 and MCP-2 are major constituents of rabbit AM and exhibit potent antibacterial and antifungal properties, they may contribute to the expression of microbicidal activity in both resident and activated states.

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Selected References

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  1. Badwey J. A., Karnovsky M. L. Active oxygen species and the functions of phagocytic leukocytes. Annu Rev Biochem. 1980;49:695–726. doi: 10.1146/annurev.bi.49.070180.003403. [DOI] [PubMed] [Google Scholar]
  2. Baggiolini M., Hirsch J. G., De Duve C. Further biochemical and morphological studies of granule fractions from rabbit heterophil leukocytes. J Cell Biol. 1970 Jun;45(3):586–597. doi: 10.1083/jcb.45.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Butterworth J., Scott F., McCrae W. M., Bain A. D. Lysosomal enzymes of cultured fibroblasts of cystic fibrosis patients. Clin Chim Acta. 1972 Aug;40(1):139–142. doi: 10.1016/0009-8981(72)90261-6. [DOI] [PubMed] [Google Scholar]
  4. COHN Z. A., WIENER E. THE PARTICULATE HYDROLASES OF MACROPHAGES. I. COMPARATIVE ENZYMOLOGY, ISOLATION, AND PROPERTIES. J Exp Med. 1963 Dec 1;118:991–1008. doi: 10.1084/jem.118.6.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. COHN Z. A., WIENER E. THE PARTICULATE HYDROLASES OF MACROPHAGES. II. BIOCHEMICAL AND MORPHOLOGICAL RESPONSE TO PARTICLE INGESTION. J Exp Med. 1963 Dec 1;118:1009–1020. doi: 10.1084/jem.118.6.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carroll S. F., Martinez R. J. Purification and properties of rabbit alveolar macrophage lysozyme. Infect Immun. 1979 May;24(2):460–467. doi: 10.1128/iai.24.2.460-467.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edwards J. E., Jr, Lehrer R. I., Stiehm E. R., Fischer T. J., Young L. S. Severe candidal infections: clinical perspective, immune defense mechanisms, and current concepts of therapy. Ann Intern Med. 1978 Jul;89(1):91–106. doi: 10.7326/0003-4819-89-1-91. [DOI] [PubMed] [Google Scholar]
  8. Franson R. C., Waite M. Lysosomal phospholipase A 1 and A 2 of normal and bacillus calmette guerin-induced alveolar macrophages. J Cell Biol. 1973 Mar;56(3):621–627. doi: 10.1083/jcb.56.3.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green G. M., Jakab G. J., Low R. B., Davis G. S. Defense mechanisms of the respiratory membrane. Am Rev Respir Dis. 1977 Mar;115(3):479–514. doi: 10.1164/arrd.1977.115.3.479. [DOI] [PubMed] [Google Scholar]
  10. Hocking W. G., Golde D. W. The pulmonary-alveolar macrophage (first of two parts). N Engl J Med. 1979 Sep 13;301(11):580–587. doi: 10.1056/NEJM197909133011104. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Leake E. S., Myrvik Q. N. Changes in morphology and in lysozyme content of free alveolar cells after the intravenous injection of killed BCG in oil. J Reticuloendothel Soc. 1968 Feb;5(1):33–53. [PubMed] [Google Scholar]
  13. Lehrer R. I., Ferrari L. G., Patterson-Delafield J., Sorrell T. Fungicidal activity of rabbit alveolar and peritoneal macrophages against Candida albicans. Infect Immun. 1980 Jun;28(3):1001–1008. doi: 10.1128/iai.28.3.1001-1008.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Patterson-Delafield J., Lehrer R. I. Preparation of rabbit alveolar macrophages in high purity and yield. J Immunol Methods. 1980;38(3-4):291–294. doi: 10.1016/0022-1759(80)90277-x. [DOI] [PubMed] [Google Scholar]
  15. Patterson-Delafield J., Martinez R. J., Lehrer R. I. Microbicidal cationic proteins in rabbit alveolar macrophages: a potential host defense mechanism. Infect Immun. 1980 Oct;30(1):180–192. doi: 10.1128/iai.30.1.180-192.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Patterson-Delafield J., Szklarek D., Martinez R. J., Lehrer R. I. Microbicidal cationic proteins of rabbit alveolar macrophages: amino acid composition and functional attributes. Infect Immun. 1981 Feb;31(2):723–731. doi: 10.1128/iai.31.2.723-731.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robinson D., Willcox P. 4-Methylumbelliferyl phosphate as a substrate for lysosomal acid phosphatase. Biochim Biophys Acta. 1969 Sep 30;191(1):183–186. doi: 10.1016/0005-2744(69)90334-9. [DOI] [PubMed] [Google Scholar]
  18. Schill W. B., Schumacher G. F. Radial diffusion in gel for micro determination of enzymes. I. Muramidase, alpha-amylase, DNase 1, RNase A, acid phosphatase, and alkaline phosphatase. Anal Biochem. 1972 Apr;46(2):502–533. doi: 10.1016/0003-2697(72)90324-7. [DOI] [PubMed] [Google Scholar]

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