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. 1981 Sep;33(3):827–833. doi: 10.1128/iai.33.3.827-833.1981

Specific receptor binding of staphylococcal enterotoxins by murine splenic lymphocytes.

S Buxser, P F Bonventre, D L Archer
PMCID: PMC350786  PMID: 7287185

Abstract

We describe a reliable assay to measure the specific binding of 125I-labeled staphylococcal enterotoxin A (SEA) by murine spleen cells. Toxin binding by lymphocytes was specific in that it was inhibited by unlabeled SEA but not by unrelated proteins. The biological activity of SEA (T-lymphocyte mitogenesis) correlated with toxin binding to splenic lymphocytes. In the presence of high concentrations of [125I]SEA, specific binding increased rapidly and approached saturation after 2 h. Toxin binding was sensitive to temperature and pH and was directly proportional to the concentration of spleen cells in the incubation mixture. We estimated that there was a single class of toxin-binding sites, which had an apparent equilibrium dissociation constant (Kd) of 8 x 10(-7) M and numbered 3,600 sites per cell. SEA and the antigenically distinct compounds staphylococcal enterotoxins B and E in excess competitively inhibited binding of [125I]SEA to mouse spleen cells. Our data suggest a common class of binding sites for the three staphylococcal enterotoxins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bornens M., Karsenti E., Avrameas S. Cooperative binding of concanavalin A to thymocytes at 4 degrees C and micro-redistribution of concanavalin A receptors. Eur J Biochem. 1976 May 17;65(1):61–69. doi: 10.1111/j.1432-1033.1976.tb10389.x. [DOI] [PubMed] [Google Scholar]
  2. Buxser S., Bonventre P. F. Staphylococcal enterotoxins fail to disrupt membrane integrity or synthetic functions of Henle 407 intestinal cells. Infect Immun. 1981 Mar;31(3):929–934. doi: 10.1128/iai.31.3.929-934.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cuatrecasas P. Membrane receptors. Annu Rev Biochem. 1974;43(0):169–214. doi: 10.1146/annurev.bi.43.070174.001125. [DOI] [PubMed] [Google Scholar]
  4. Ferguson A. Intraepithelial lymphocytes of the small intestine. Gut. 1977 Nov;18(11):921–937. doi: 10.1136/gut.18.11.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gartler S. M. Apparent Hela cell contamination of human heteroploid cell lines. Nature. 1968 Feb 24;217(5130):750–751. doi: 10.1038/217750a0. [DOI] [PubMed] [Google Scholar]
  6. Guy-Grand D., Griscelli C., Vassalli P. The mouse gut T lymphocyte, a novel type of T cell. Nature, origin, and traffic in mice in normal and graft-versus-host conditions. J Exp Med. 1978 Dec 1;148(6):1661–1677. doi: 10.1084/jem.148.6.1661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hook W. A., Dougherty S. F., Oppenheim J. J. Release of histamine from hamster mast cells by concanavalin A and phytohemagglutinin. Infect Immun. 1974 May;9(5):903–908. doi: 10.1128/iai.9.5.903-908.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kaplan J. Staphylococcal enterotoxin B induced release of macrophage migration inhibition factor from normal lymphocytes. Cell Immunol. 1972 Feb;3(2):245–252. doi: 10.1016/0008-8749(72)90163-3. [DOI] [PubMed] [Google Scholar]
  9. Kauffman P. E., Johnson H. M. Stability of 125I-labeled staphylococcal enterotoxins in solid-phase radioimmunoassay. Appl Microbiol. 1975 Jun;29(6):776–779. doi: 10.1128/am.29.6.776-779.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Langford M. P., Stanton G. J., Johnson H. M. Biological effects of staphylococcal enterotoxin A on human peripheral lymphocytes. Infect Immun. 1978 Oct;22(1):62–68. doi: 10.1128/iai.22.1.62-68.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Novogrodsky A., Katchalski E. Lymphocyte transformation induced by concanavalin A and its reversion by methyl-alpha-D-mannopyranoside. Biochim Biophys Acta. 1971 Jan 28;228(2):579–583. doi: 10.1016/0005-2787(71)90064-5. [DOI] [PubMed] [Google Scholar]
  12. Peavy D. L., Adler W. H., Smith R. T. The mitogenic effects of endotoxin and staphylococcal enterotoxin B on mouse spleen cells and human peripheral lymphocytes. J Immunol. 1970 Dec;105(6):1453–1458. [PubMed] [Google Scholar]
  13. Pinto M., Torten M., Birnbaum S. C. Suppression of the in vivo humoral and cellular immune response by staphylococcal enterotoxin B (SEB). Transplantation. 1978 Jun;25(6):320–323. doi: 10.1097/00007890-197806000-00008. [DOI] [PubMed] [Google Scholar]
  14. Prujansky A., Ravid A., Sharon N. Cooperativity of lectin binding to lymphocytes, and its relevance to mitogenic stimulation. Biochim Biophys Acta. 1978 Mar 21;508(1):137–146. doi: 10.1016/0005-2736(78)90195-5. [DOI] [PubMed] [Google Scholar]
  15. Schaeffer W. I., Gabliks J., Calitis R. Interaction of staphylococcal enterotoxin B with cell cultures of human embryonic intestine. J Bacteriol. 1966 Jan;91(1):21–26. doi: 10.1128/jb.91.1.21-26.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schantz E. J., Roessler W. G., Wagman J., Spero L., Dunnery D. A., Bergdoll M. S. Purification of staphylococcal enterotoxin B. Biochemistry. 1965 Jun;4(6):1011–1016. doi: 10.1021/bi00882a005. [DOI] [PubMed] [Google Scholar]
  17. Schantz E. J., Roessler W. G., Woodburn M. J., Lynch J. M., Jacoby H. M., Silverman S. J., Gorman J. C., Spero L. Purification and some chemical and physical properties of staphylococcal enterotoxin A. Biochemistry. 1972 Feb 1;11(3):360–366. doi: 10.1021/bi00753a009. [DOI] [PubMed] [Google Scholar]
  18. Smith B. G., Johnson H. M. The effect of staphylococcal enterotoxins on the primary in vitro immune response. J Immunol. 1975 Aug;115(2):575–578. [PubMed] [Google Scholar]
  19. Sullivan T. J., Greene W. C., Parker C. W. Concanavalin A-induced histamine release from normal rat mast cells. J Immunol. 1975 Jul;115(1):278–282. [PubMed] [Google Scholar]
  20. Taranta A. Lymphocyte mitogens of staphylococcal origin. Ann N Y Acad Sci. 1974 Jul 31;236(0):362–375. doi: 10.1111/j.1749-6632.1974.tb41503.x. [DOI] [PubMed] [Google Scholar]
  21. Warren J. R., Leatherman D. L., Metzger J. F. Evidence for cell-receptor activity in lymphocyte stimulation by staphylococcal enterotoxin. J Immunol. 1975 Jul;115(1):49–53. [PubMed] [Google Scholar]

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