Abstract
Introduction
The optimal stage for dipeptidyl peptidase-4 (DPP-4) inhibitor therapy in the course of type 2 diabetes mellitus (T2DM) is still under discussion, with often a perception that treatment with these agents may be less beneficial with increasing disease progression, due to loss of beta-cell function, and with increasing insulin resistance (IR), where beta-cell function is less prominent. This work, therefore, aimed to assess the impact of such factors on the efficacy of the DPP-4 inhibitor, vildagliptin, in add-on therapy to metformin.
Methods
A pooled analysis of 24-week efficacy data of vildagliptin 50 mg twice daily (b.i.d.) (n = 2,478) from four add-on to metformin studies was performed. Analyses for changes in hemoglobin A1c (HbA1c) were stratified according to baseline IR stage (homeostasis model assessment [Homa IR] <5, ≥5), body mass index (BMI) (<27, ≥27 to <30, ≥30 kg/m2), T2DM duration (0 to <1, ≥1 to <5, ≥5 years), and duration of metformin use (0 to <1, ≥1 to <5, ≥5 years). Data from patients treated with sulfonylureas (SUs) (n = 2,010) in the pooled studies are provided as reference.
Results
Patients in the vildagliptin and SU groups had mean age, HbA1c, BMI, Homa IR, duration of T2DM and metformin use of 58 years, 7.7%, 32 kg/m2, 4.3, 5.9 years and 3.0 years, respectively. Reductions from baseline in HbA1c with vildagliptin were very similar across Homa IR (mean 2.8 and 8.6), BMI (mean 24.9, 28.5, and 35.3 kg/m2), T2DM duration (mean 0.6, 2.9, and 9.7 years), and duration of metformin use (mean 0.6, 2.6, and 7.9 years) categories, showing significant drops in HbA1c of approximately −0.7% (baseline 7.7%). The results in patients receiving SUs were comparable to those seen in the vildagliptin group.
Conclusion
Vildagliptin add-on therapy to metformin was efficacious independent of IR stage and BMI, as well as disease duration and duration of prior metformin use, indicating that, contrary to a not uncommon perception, more obese patients and patients with long-standing T2DM can benefit from treatment with the DPP-4 inhibitor, vildagliptin.
Keywords: Body mass index, Dipeptidyl peptidase-4, Disease duration, Efficacy, Hemoglobin A1c, Insulin resistance, Type 2 diabetes, Vildagliptin
Introduction
Type 2 diabetes mellitus (T2DM) is a progressive disease, with increasing insulin resistance (IR) (tied to obesity and/or adiposity) and deteriorating beta-cell function being key pathophysiologic defects in the development and worsening of hyperglycemia.
Based on the understanding of the pathogenesis of T2DM, several distinct pharmacological therapies have been developed. Within this armamentarium, dipeptidyl peptidase-4 (DPP-4) inhibitors are the most recent addition to the oral treatment options available in clinical practice for the treatment of T2DM.
The DPP-4 enzyme rapidly inactivates the incretin hormones, glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP) [1], which play a crucial role in glucose homeostasis [2, 3]. DPP-4 inhibitors bind to the catalytic site of DPP-4, resulting in prolonged inhibition of the enzyme, thus, raising levels of intact GLP-1 and GIP [4]. The resulting improvement in islet dysfunction is a key mechanism underlying the glucose-lowering mechanism of DPP-4 inhibitors [4]. There is also a potential beta-cell preservation effect, as judged from rodent models, in which an increase in beta-cell mass through stimulation of neogenesis and proliferation, as well as an inhibition of apoptosis, has been seen with GLP-1/DPP-4 inhibitors [5, 6].
While DPP-4 inhibitor therapy has been studied in a variety of clinical study settings [7–10], the optimal stage for their use in the course of T2DM is still under debate. Based on the known effects on beta-cell function, there is frequently a perception that treatment with these agents may be less efficacious with increasing disease progression, due to loss of beta-cell function, and with increasing IR, where beta-cell function is proportionally less important.
The present paper, therefore, aimed to evaluate the impact of such factors (i.e., T2DM duration and duration of previous therapy, and IR and body mass index [BMI]) on the efficacy of the DPP-4 inhibitor, vildagliptin, using a pooled database of studies in add-on therapy to metformin. Vildagliptin is a DPP-4 inhibitor that has been extensively studied in a large clinical program, with proven efficacy and a well-established safety profile [8, 11–14]. The present paper reports the results of these new pooled subgroup analyses with vildagliptin.
Methods
Populations
The efficacy analyses included all randomized, double-blind, controlled, parallel-group add-on to metformin studies with vildagliptin 50 mg twice daily (b.i.d.) (the highest approved and most commonly used dose of the drug). A total of four studies were pooled (termed ‘pooled add-on to metformin population’). The efficacy population comprised all patients who were included in the respective primary efficacy populations of the individual studies [15–18]. Data collected in the first 24 weeks of treatment from these studies were included in the analyses. Details of the study designs are summarized in Appendix Table 3 and the individual study publications [15–18].
Assessments
Hemoglobin A1c (HbA1c) was measured regularly in all studies. The laboratory assessments were performed by central laboratories: Bioanalytical Research Corporation-EU (Ghent, Belgium), Diabetes Diagnostics Laboratory (Columbia, MO, US), and Covance (Geneva, Switzerland, Singapore, or Indianapolis, IN, US).
Homeostasis model assessment IR (Homa IR) was calculated based on the following formula: Homa IR = (fasting insulin [μU/mL]) × (fasting glucose [mmol/L])/22.5. Glomerular filtration rate (GFR) was estimated by the Modification of Diet in Renal Disease study method (MDRD) [19].
Data Analysis
The total number of patients, and demography and baseline characteristics were recorded.
Efficacy analyses were stratified according to baseline IR stage (Homa IR <5 and ≥5), BMI (<27, ≥27 to <30, ≥30 kg/m2), T2DM duration (0 to <1, ≥1 to <5, ≥5 years), and duration of metformin use (0 to <1, ≥1 to <5, ≥5 years). Mean changes from baseline in HbA1c at the week 24 endpoint in the defined subgroups were analyzed using last observation carried forward (LOCF). Within-group comparisons (endpoint vs. baseline) were made using one-sample t test at a significance level of 0.05.
Efficacy data are reported for vildagliptin 50 mg b.i.d. (n = 2,478), with data from patients treated with a sulfonylurea (SU) in the pooled studies being provided as reference (n = 2,010). While the SU group had a comparable number of patients to the vildagliptin group, the two other comparator groups in the studies (placebo and thiazolidinedione [TZD]) comprised only 2.6% and 5.7%, respectively, of patients in the pooled population; thus, not representing meaningful reference groups to be analyzed.
Ethics and Good Clinical Practice
All study participants provided written informed consent. All protocols were approved by the independent ethics committee/institutional review board at each study site or country. All studies were conducted using good clinical practice and in accordance with the Declaration of Helsinki.
Results
Demography
Table 1 summarizes the demographic and baseline characteristics of patients in the pooled add-on to metformin population. The population studied was representative of a broad spectrum of T2DM patients, with a mean age of 58 years, mean HbA1c of 7.7%, mean fasting blood glucose (FPG) of 9.6 mmol/L, mean BMI of 32 kg/m2, and mean Homa IR of 4.3, respectively. The mean duration of diagnosed T2DM was 5.9 years, with a mean duration of metformin use of 3.0 years. Approximately 30% of patients had renal impairment (mostly mild), >65% had hypertension, and about 50% had dyslipidemia. The majority of patients (>80%) were Caucasian. The demographic and baseline characteristics were comparable across the vildagliptin group and the SU reference group.
Table 1.
Demography and baseline characteristics (pooled add-on to metformin population)
| Mean ± SD or n (%) | Vilda 50 mg b.i.d. + metformin | SU + metformin |
|---|---|---|
| n | 2,478 | 2,010 |
| Age (years) | 57.5 ± 9.4 | 58.0 ± 9.5 |
| ≥65 | 638 (25.7) | 595 (29.6) |
| Sex | ||
| Male | 1,347 (54.4) | 1,070 (53.2) |
| Female | 1,131 (45.6) | 940 (46.8) |
| Race | ||
| Caucasian | 2,089 (84.3) | 1,691 (84.1) |
| Black | 43 (1.7) | 25 (1.2) |
| Asian | 103 (4.2) | 86 (4.3) |
| Hispanic/Latino | 227 (9.2) | 190 (9.5) |
| All other | 16 (0.6) | 18 (0.9) |
| n | 2,477 | 2,010 |
| HbA1c (%) | 7.7 ± 1.0 | 7.6 ± 0.9 |
| n | 2,475 | 2,009 |
| FPG (mmol/L) | 9.7 ± 2.5 | 9.5 ± 2.5 |
| n | 2,472 | 2,003 |
| BMI (kg/m2) | 31.9 ± 5.3 | 31.5 ± 5.2 |
| ≤27 | 465 (18.8) | 421 (20.9) |
| ≤30 | 1,022 (41.2) | 878 (43.7) |
| >30 | 1,450 (58.5) | 1,125 (56.0) |
| >35 | 651 (26.3) | 468 (23.3) |
| Not recorded | 6 (0.2) | 7 (0.3) |
| n | 2,121 | 1,703 |
| Homa IR | 4.4 ± 4.2 | 4.2 ± 3.7 |
| <5 | 1,519 (61.3) | 1,267 (63.0) |
| ≥5 | 602 (24.3) | 436 (21.7) |
| Not recorded | 357 (14.4) | 307 (15.3) |
| n | 2,478 | 2,010 |
| Duration of T2DM (years) | 5.9 ± 5.1 | 6.0 ± 5.1 |
| 0 to <1 | 237 (9.6) | 202 (10.0) |
| ≥1 to <5 | 1,079 (43.5) | 850 (42.3) |
| ≥5 | 1,162 (46.9) | 958 (47.7) |
| n | 2,477 | 2,010 |
| Duration of metformin use (years) | 3.0 ± 3.0 | 3.0 ± 2.9 |
| 0 to <1 | 713 (28.8) | 591 (29.4) |
| ≥1 to <5 | 1,294 (52.2) | 1,047 (52.1) |
| ≥5 | 470 (19.0) | 372 (18.5) |
| Not recorded | 1 (0.0) | 0 (0.0) |
| n | 2,478 | 2,010 |
| Medical history | ||
| GFR (MDRD) (mL/min) per (1.73 m2) | ||
| Normal | 1,713 (69.1) | 1,367 (68.0) |
| Mild (≥50, ≤80) | 739 (29.8) | 626 (31.1) |
| Moderate (≥30, ≤50) | 23 (0.9) | 17 (0.8) |
| Severe (<30) | 2 (0.1) | 0 (0.0) |
| Not recorded | 1 (0.0) | 0 (0.0) |
| Hypertension | 1,586 (64.0) | 1,375 (68.4) |
| Dyslipidemia | 1,279 (51.6) | 992 (49.4) |
b.i.d. twice daily, BMI body mass index, FPG fasting plasma glucose, GFR glomerular filtration rate, HbA 1c hemoglobin A1c, Homa IR homeostasis model assessment insulin resistance, MDRD Modification of Diet in Renal Disease, SU sulfonylurea, T2DM type 2 diabetes mellitus, Vilda vildagliptin
Efficacy by Subgroups
Table 2 displays mean changes from baseline to week 24 in HbA1c with vildagliptin 50 mg b.i.d. according to Homa IR, BMI, T2DM duration, and duration of metformin use in the pooled add-on to metformin population.
Table 2.
Efficacy (HbA1c [%]) by Homa IR, BMI, T2DM duration and duration of metformin use
| Vilda 50 mg b.i.d. + metformin | SU + metformin | |||
|---|---|---|---|---|
| BL | Mean ∆ | BL | Mean ∆ | |
| BL Homa IR | ||||
| n | 1,507 | 1,237 | ||
| <5 (mean = 2.8/2.7) | 7.63 ± 0.02 | −0.65 ± 0.02* | 7.51 ± 0.03 | −0.76 ± 0.02* |
| n | 598 | 428 | ||
| ≥5 (mean = 8.6/8.3) | 8.07 ± 0.05 | −0.68 ± 0.04* | 7.80 ± 0.05 | −0.77 ± 0.05* |
| BL BMI | ||||
| n | 444 | 398 | ||
| <27 kg/m2 (mean = 24.9/25.1) | 7.80 ± 0.05 | −0.67 ± 0.04* | 7.66 ± 0.05 | −0.80 ± 0.04* |
| n | 556 | 453 | ||
| ≥27 to <30 kg/m2 (mean = 28.5/28.4) | 7.74 ± 0.04 | −0.67 ± 0.04* | 7.59 ± 0.04 | −0.79 ± 0.04* |
| n | 1,446 | 1,106 | ||
| ≥30 kg/m2 (mean = 35.3/35.1) | 7.72 ± 0.03 | −0.63 ± 0.02* | 7.58 ± 0.03 | −0.73 ± 0.03* |
| BL T2DM duration | ||||
| n | 236 | 198 | ||
| 0 to <1 year (mean = 0.6/0.6) | 7.47 ± 0.06 | −0.64 ± 0.05* | 7.32 ± 0.06 | −0.73 ± 0.06* |
| n | 1,061 | 829 | ||
| ≥1 to <5 years (mean = 2.9/2.9) | 7.65 ± 0.03 | −0.60 ± 0.03* | 7.49 ± 0.03 | −0.71 ± 0.03* |
| n | 1,155 | 936 | ||
| ≥5 years (mean = 9.7/9.8) | 7.88 ± 0.03 | −0.69 ± 0.03* | 7.75 ± 0.03 | −0.80 ± 0.03* |
| BL duration of metformin use | ||||
| n | 709 | 580 | ||
| 0 to <1 year (mean = 0.6/0.6) | 7.70 ± 0.04 | −0.63 ± 0.03* | 7.53 ± 0.04 | −0.75 ± 0.04* |
| n | 1,274 | 1,022 | ||
| ≥1 to <5 years (mean = 2.6/2.6) | 7.70 ± 0.03 | −0.63 ± 0.02* | 7.58 ± 0.03 | −0.74 ± 0.03* |
| n | 468 | 361 | ||
| ≥5 years (mean = 7.9/7.7) | 7.93 ± 0.05 | −0.70 ± 0.04* | 7.76 ± 0.05 | −0.82 ± 0.05* |
Data are mean ± SE
b.i.d. twice daily, BL baseline, BMI body mass index, HbA 1c hemoglobin A1c, Mean ∆ mean change from BL, n number of patients with observations at both baseline and endpoint, SU sulfonylurea, T2DM type 2 diabetes mellitus, Vilda vildagliptin
* P < 0.0001
The influence of IR on the efficacy of vildagliptin was assessed by stratification according to two baseline Homa IR categories, Homa IR <5 (mean = 2.8) and Homa IR ≥5 (mean = 8.6). In addition, three BMI categories were analyzed, BMI <27 kg/m2 (mean = 24.9 kg/m2), ≥27 to <30 kg/m2 (mean = 28.5 kg/m2), and ≥30 kg/m2 (mean = 35.3 kg/m2). HbA1c was reduced to a very similar extent independent of baseline Homa IR or BMI category. For example, HbA1c (± SE) was reduced by −0.67 ± 0.04% (baseline 7.80 ± 0.05%), −0.67 ± 0.04% (baseline 7.74 ± 0.04%), and −0.63 ± 0.02% (baseline 7.72 ± 0.03%) in the BMI subgroups of <27, ≥27 to <30, and ≥27 kg/m2, respectively.
The impact of disease progression on the efficacy of vildagliptin was evaluated by stratification according to three T2DM duration categories, 0 to <1 year (mean = 0.6 years), ≥1 to <5 years (mean = 2.9 years), and ≥5 years (mean = 9.7 years). In addition, the duration of metformin use was considered, with categories of 0 to <1 year (mean = 0.6 years), ≥1 to <5 years (mean = 2.6 years), and ≥5 years (mean = 7.9 years). Patients with shorter and longer duration of T2DM and metformin use had very similar decreases in HbA1c. For example, HbA1c (±SE) was reduced by −0.64 ± 0.05% (baseline 7.47 ± 0.06%), −0.60 ± 0.03% (baseline 7.65 ± 0.03%), and −0.69 ± 0.03% (baseline 7.88 ± 0.03%) in the T2DM duration subgroups of 0 to <1, ≥1 to <5, and ≥5 years, respectively.
Table 2 also displays efficacy results in the different sub-categories for the SU reference group. The results in patients receiving a SU were comparable to those seen in the vildagliptin group, with similar HbA1c reductions across the different Homa IR, BMI, T2DM duration, and duration of metformin use subgroups.
Discussion
The present paper evaluated the impact of IR, BMI, disease duration, and duration of metformin use on the efficacy of vildagliptin in patients with T2DM treated with metformin. The pooled analysis found similar efficacy of vildagliptin independent of these factors, indicating that patients with higher IR, more obese patients, and patients with long-standing T2DM can benefit from treatment with the DPP-4 inhibitor, vildagliptin, in combination with metformin.
These data can provide relevant information for the use of vildagliptin in clinical practice, in particular given the not uncommon perception that treatment with DPP-4 inhibitors may be less beneficial with increasing disease progression, due to loss of beta-cell function, and with increasing IR, where beta-cell function is proportionally less important. The notion of DPP-4 inhibitors being more beneficial in patients with shorter disease duration may at least be in part related to the promise from animal models [5, 6] that these incretin-based therapies may be able to interfere with disease progression if used as an early intervention, when sufficient beta-cell function/mass can be still be preserved or restored, which raised particular attention when these drugs initially became available. On the other hand, consistent with the findings of the present report, vildagliptin has meanwhile been shown to be efficacious across a wide disease spectrum, including add-on therapy to insulin as well as patients with renal impairment, both involving patients with long-standing T2DM (mean T2DM duration 13–17 years) [20–23].
In this context, it is important to keep in mind that vildagliptin, in addition to improving beta-cell function, also enhances alpha-cell function (i.e., suppresses inappropriate glucagon secretion as shown after oral glucose and meal ingestion [24–26]), with the changes in islet hormone (i.e., increased insulin and reduced glucagon) secretion leading to a profound effect to suppress hepatic glucose production [27]. Furthermore, vildagliptin is associated with reduced insulin resistance due to reduced lipotoxicity [28, 29]. Thus, a variety of pancreatic and extra-pancreatic effects are active to support the efficacy of vildagliptin [4].
Similar results to those seen with vildagliptin plus metformin were also seen in the group of patients treated with SUs in combination with metformin, who were analyzed as a reference group. The common perception is that both DPP-4 inhibitors and SUs tend to be more efficacious in lean than obese individuals and the reverse is true for TZDs. The data in this study do not support this perception about vildagliptin and SUs, and the authors are unaware of any data that does. In contrast, addition of the TZD, rosiglitazone, to metformin was indeed shown to be most efficacious in obese, insulin-resistant patients, as expected from the mechanism of action [30]. Similar results were also seen with pioglitazone added to SU therapy in a small Japanese study; HbA1c was decreased considerably more in the Homa IR ≥4 category compared to the <4 subgroup [31]. The current study does not address the question of whether a longer duration of SU use is associated with a reduced response, but indicates that addition of SUs to patients with a longer duration of T2DM is not associated with reduced efficacy. This is in contrast to one open-label study of glimepiride monotherapy [32], which showed a weak association of longer duration of diabetes with the likelihood of being a nonresponder (odds ratio [OR] = 1.096); however, the study was confounded by a strong correlation with previous oral antidiabetic drug (OAD) treatment (OR = 2.506).
In light of the applicability of the reported results, it is important to note that each of the categories investigated included a broad range of vildagliptin-treated patients, with means of IR categories from 2.8 to 8.6, of BMI from 24.9 to 35.3 kg/m2, and of disease duration from <1 year to nearly 10 years (overall range 0.1–40.1 years). Another important factor for the interpretation of the subgroup results is that HbA1c levels were well matched between the different categories, which is an important consideration given that HbA1c levels strongly influence response to treatment. Since metformin was used as a background drug, some limitations of the clinical applicability of the present results to other indications cannot be ruled out, and it will be of interest to investigate whether similar results will be seen with vildagliptin in combination with other drugs, as well as in monotherapy. While no such data are currently available for the subgroups of disease duration or IR stage, in a study with vildagliptin monotherapy in patients with mild hyperglycemia, the same treatment effect was seen in patients with baseline BMI < 30 and ≥30 kg/m2 [33].
Conclusion
Taken together, the present pooled analysis provides a comprehensive assessment of the efficacy of vildagliptin in subgroups of IR stage and BMI, as well as disease duration and duration of prior metformin use, showing similar efficacy of vildagliptin across a wide spectrum of these categories.
Acknowledgments
The authors acknowledge the patients, investigators, and staff at participating sites for all the studies. This work was funded by Novartis Pharmaceuticals Corporation. Dr. Schweizer is the guarantor for this article, and takes responsibility for the integrity of the work as a whole.
Conflict of interest
All authors are employees of Novartis.
Open Access
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Appendix
Table 3.
Vildagliptin add-on to metformin studies contributing to analyses
| Study No. | Study description | Randomized patients | Treatment duration (weeks) | Publication | |
|---|---|---|---|---|---|
| 1 | Placebo-controlled efficacy/safety study in T2DM patients inadequately controlled with metformin (HbA1c 7.5–11%) | 544 | 24 | [15] | |
| 2 | Active-controlled (glimepiride) long-term efficacy/safety study in T2DM patients treated with metformin (HbA1c >6.5–8.5%) | 3,118 | ≥104 | [16, 34] | |
| 3 | Active-controlled (gliclazide) long-term efficacy/safety study in T2DM patients inadequately controlled with metformin (HbA1c 7.5–11%) | 1,007 | 52 | [17] | |
| 4 | Active-controlled (pioglitazone) long-term efficacy/safety study in T2DM patients inadequately controlled with metformin (HbA1c 7.5–11%) | 576 | 52 | [18, 35] | |
HbA 1c hemoglobin A1c, T2DM type 2 diabetes mellitus
References
- 1.Mentlein R, Gallwitz B, Schmidt WE. Dipeptidyl-peptidase IV hydrolyses gastric inhibitory polypeptide, glucagon-like peptide-1(7–36)amide, peptide histidine methionine and is responsible for their degradation in human serum. Eur J Biochem. 1993;214:829–835. doi: 10.1111/j.1432-1033.1993.tb17986.x. [DOI] [PubMed] [Google Scholar]
- 2.Creutzfeldt W. The incretin concept today. Diabetologia. 1979;16:75–85. doi: 10.1007/BF01225454. [DOI] [PubMed] [Google Scholar]
- 3.Creutzfeldt W, Nauck M. Gut hormones and diabetes mellitus. Diabetes Metab Rev. 1992;8:149–177. doi: 10.1002/dmr.5610080206. [DOI] [PubMed] [Google Scholar]
- 4.Ahren B, Schweizer A, Dejager S, Villhauer EB, Dunning BE, Foley JE. Mechanisms of action of the dipeptidyl peptidase-4 inhibitor vildagliptin in humans. Diabetes Obes Metab. 2011;13:775–783. doi: 10.1111/j.1463-1326.2011.01414.x. [DOI] [PubMed] [Google Scholar]
- 5.Perfetti R, Hui H. The role of GLP-1 in the life and death of pancreatic beta cells. Horm Metab Res. 2004;36:804–810. doi: 10.1055/s-2004-826167. [DOI] [PubMed] [Google Scholar]
- 6.Duttaroy A, Voelker F, Merriam K, et al. The DPP-4 inhibitor vildagliptin increases pancreatic beta cell mass in neonatal rats. Eur J Pharmacol. 2011;650:703–707. doi: 10.1016/j.ejphar.2010.10.062. [DOI] [PubMed] [Google Scholar]
- 7.Dhillon S, Weber J. Saxagliptin. Drugs. 2009;69:2103–2114. doi: 10.2165/11201170-000000000-00000. [DOI] [PubMed] [Google Scholar]
- 8.Keating GM. Vildagliptin—a review of its use in type 2 diabetes mellitus. Drugs. 2010;70:2089–2112. doi: 10.2165/11206370-000000000-00000. [DOI] [PubMed] [Google Scholar]
- 9.Dhillon S. Sitagliptin: a review of its use in the management of type 2 diabetes mellitus. Drugs. 2010;70:489–512. doi: 10.2165/11203790-000000000-00000. [DOI] [PubMed] [Google Scholar]
- 10.Scott LJ. Linagliptin: in type 2 diabetes mellitus. Drugs. 2011;71:611–624. doi: 10.2165/11207400-000000000-00000. [DOI] [PubMed] [Google Scholar]
- 11.Ligueros-Saylan M, Foley JE, Schweizer A, Couturier A, Kothny W. An assessment of adverse effects of vildagliptin versus comparators on the liver, the pancreas, the immune system, the skin and in patients with impaired renal function from a large pooled database of Phase II and III clinical trials. Diabetes Obes Metab. 2010;12:495–509. doi: 10.1111/j.1463-1326.2010.01214.x. [DOI] [PubMed] [Google Scholar]
- 12.Schweizer A, Dejager S, Foley JE, Kothny W. Assessing the general safety and tolerability of vildagliptin: value of pooled analyses from a large safety database versus evaluation of individual studies. Vasc Health Risk Manag. 2011;7:49–57. doi: 10.2147/VHRM.S16925. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Schweizer A, Dejager S, Foley JE, Couturier A, Ligueros-Saylan M, Kothny W. Assessing the cardio-cerebrovascular safety of vildagliptin: meta-analysis of adjudicated events from a large Phase III type 2 diabetes population. Diabetes Obes Metab. 2010;12:485–494. doi: 10.1111/j.1463-1326.2010.01215.x. [DOI] [PubMed] [Google Scholar]
- 14.Schweizer A, Dejager S, Foley JE, Shao Q, Kothny W. Clinical experience with vildagliptin in the management of type 2 diabetes in a patient population ≥75 years: a pooled analysis from a database of clinical trials. Diabetes Obes Metab. 2011;13:55–64. doi: 10.1111/j.1463-1326.2010.01325.x. [DOI] [PubMed] [Google Scholar]
- 15.Bosi E, Camisasca RP, Collober C, Rochotte E, Garber AJ. Effects of vildagliptin on glucose control over 24 weeks in patients with type 2 diabetes inadequately controlled with metformin. Diabetes Care. 2007;30:890–895. doi: 10.2337/dc06-1732. [DOI] [PubMed] [Google Scholar]
- 16.Ferrannini E, Fonseca V, Zinman B, et al. Fifty-two-week efficacy and safety of vildagliptin versus glimepiride in patients with type 2 diabetes mellitus inadequately controlled on metformin monotherapy. Diabetes Obes Metab. 2009;11:157–166. doi: 10.1111/j.1463-1326.2008.00994.x. [DOI] [PubMed] [Google Scholar]
- 17.Filozof C, Gautier J-F. A comparison of efficacy and safety of vildagliptin and gliclazide in combination with metformin in patients with type 2 diabetes inadequately controlled with metformin alone: a 52-week, randomized study. Diabet Med. 2010;27:318–326. doi: 10.1111/j.1464-5491.2010.02938.x. [DOI] [PubMed] [Google Scholar]
- 18.Bolli G, Dotta F, Rochotte E, Cohen SE. Efficacy and tolerability of vildagliptin versus pioglitazone when added to metformin: a 24-week, randomized, double-blind study. Diabetes Obes Metab. 2008;10:82–90. doi: 10.1111/j.1463-1326.2007.00820.x. [DOI] [PubMed] [Google Scholar]
- 19.Rigalleau V, Lasseur C, Perlemoine C, et al. Estimation of glomerular filtration rate in diabetic subjects. Cockcroft formula or modification of diet in renal disease study equation? Diabetes Care. 2005;28:838–843. doi: 10.2337/diacare.28.4.838. [DOI] [PubMed] [Google Scholar]
- 20.Fonseca V, Schweizer A, Albrecht D, Baron MA, Chang I, Dejager S. Addition of vildagliptin to insulin improves glycemic control in type 2 diabetes. Diabetologia. 2007;50:1148–1155. doi: 10.1007/s00125-007-0633-0. [DOI] [PubMed] [Google Scholar]
- 21.Lukashevich V, Kozlovski P, Foley J, Kothny W. Vildagliptin combined with insulin reduces HbA1c without increasing risk of hypoglycemia and weight gain in patients with type 2 diabetes mellitus. Poster to be presented at: The 72nd Scientific Sessions of the American Diabetes Association; June 8–12, 2012; Philadelphia, PA, USA.
- 22.Lukashevich V, Schweizer A, Shao Q, Groop P-H, Kothny W. Safety and efficacy of vildagliptin versus placebo in patients with type 2 diabetes and moderate or severe renal impairment: a prospective 24-week randomized placebo-controlled trial. Diabetes Obes Metab. 2011;13:947–954. doi: 10.1111/j.1463-1326.2011.01467.x. [DOI] [PubMed] [Google Scholar]
- 23.Dejager S, Schweizer A. Incretin therapies in the management of patients with type 2 diabetes mellitus and renal impairment. Hosp Pract (Minneap). 2012;40:7–21. doi: 10.3810/hp.2012.04.965. [DOI] [PubMed] [Google Scholar]
- 24.He YL, Serra D, Wang Y, et al. Pharmacokinetics and pharmacodynamics of vildagliptin in patients with type 2 diabetes mellitus. Clin Pharmacokinet. 2007;46:577–588. doi: 10.2165/00003088-200746070-00003. [DOI] [PubMed] [Google Scholar]
- 25.Ahren B, Landin-Olsson M, Jansson P-A, Svensson M, Holmes D, Schweizer A. Inhibition of dipeptidylpeptidase-4 reduces glycemia, sustains insulin levels and reduces glucagon levels in type 2 diabetes. J Clin Endocrinol Metab. 2004;89:2078–2084. doi: 10.1210/jc.2003-031907. [DOI] [PubMed] [Google Scholar]
- 26.Hare KJ, Vilsboll T, Asmar M, Deacon CF, Knop FK, Holst JJ. The glucagonostatic and insulinotropic effects of glucagon-like peptide 1 contribute equally to its glucose-lowering action. Diabetes. 2010;59:1765–1770. doi: 10.2337/db09-1414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 27.Balas B, Baig MR, Watson C, et al. The dipeptidylpeptidase-4 inhibitor vildagliptin suppresses endogenous glucose production and enhances islet function after single-dose administration in type 2 diabetic patients. J Clin Endocrinol Metab. 2007;92:1249–1255. doi: 10.1210/jc.2006-1882. [DOI] [PubMed] [Google Scholar]
- 28.Azuma K, Radikova Z, Mancino J, et al. Measurements of islet function and glucose metabolism with the dipeptidyl peptidase 4 inhibitor vildagliptin in patients with type 2 diabetes. J Clin Endocrinol Metab. 2008;93:459–464. doi: 10.1210/jc.2007-1369. [DOI] [PubMed] [Google Scholar]
- 29.Boschmann M, Engeli S, Dobberstein K, et al. Dipeptidyl-peptidase-IV inhibition augments postprandial lipid mobilization and oxidation in type 2 diabetic patients. J Clin Endocrinol Metab. 2009;94:846–852. doi: 10.1210/jc.2008-1400. [DOI] [PubMed] [Google Scholar]
- 30.Jones TA, Sautter M, van Gaal LF, Jones NP. Addition of rosiglitazone to metformin is most effective in obese, insulin-resistant patients with type 2 diabetes. Diabetes Obes Metab. 2003;5:163–170. doi: 10.1046/j.1463-1326.2003.00258.x. [DOI] [PubMed] [Google Scholar]
- 31.Nagasaka S, Aiso Y, Yoshizawa K, Ishibashi S. Comparison of pioglitazone and metformin efficacy using homeostasis model assessment. Diabet Med. 2004;21:136–141. doi: 10.1111/j.1464-5491.2004.01083.x. [DOI] [PubMed] [Google Scholar]
- 32.Charpentier G, Vaur L, Halimi S, et al. Predictors of response to glimepiride in patients with type 2 diabetes mellitus. Diabetes Metab. 2001;27:563–571. [PubMed] [Google Scholar]
- 33.Scherbaum WA, Schweizer A, Mari A, et al. Efficacy and tolerability of vildagliptin in drug-naïve patients with type 2 diabetes and mild hyperglycemia. Diabetes Obes Metab. 2008;10:675–682. doi: 10.1111/j.1463-1326.2008.00850.x. [DOI] [PubMed] [Google Scholar]
- 34.Matthews DR, Dejager S, Ahren B, et al. Vildagliptin add-on to metformin produces similar efficacy and reduced hypoglycaemic risk compared with glimepiride, with no weight gain: results from a 2-year study. Diabetes Obes Metab. 2010;12:780–789. doi: 10.1111/j.1463-1326.2010.01233.x. [DOI] [PubMed] [Google Scholar]
- 35.Bolli G, Dotta F, Colin L, Minic B, Goodman M. Comparison of vildagliptin and pioglitazone in patients with type 2 diabetes inadequately controlled with metformin. Diabetes Obes Metab. 2009;11:589–595. doi: 10.1111/j.1463-1326.2008.01023.x. [DOI] [PubMed] [Google Scholar]