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. 1981 Oct;34(1):6–10. doi: 10.1128/iai.34.1.6-10.1981

Interactions between Histoplasma capsulatum and macrophages from normal and treated mice: comparison of the mycelial and yeast phases in alveolar and peritoneal macrophages.

C L Kimberlin, A R Hariri, H O Hempel, N L Goodman
PMCID: PMC350811  PMID: 7298193

Abstract

Interactions between macrophages (alveolar and peritoneal) from normal, vaccinated (with heat-killed yeast cells), and Mycobacterium bovis BCG-treated mice and the mycelial and yeast phases of Histoplasma capsulatum were observed. Phagocytosis of microconidia, small hyphal fragments, and yeast cells occurred 4 to 6 h after the infection of macrophage cultures. Conversion to the yeast phase began at 6 to 7 h and was complete after a 72-h incubation at 37 degrees C. Macrophages surrounded and adhered to macroconidia and large hyphal elements. More macrophages (65 to 68%) from BCG-treated mice contained fungi at 24 h than did macrophages from normal or vaccinated mice. Although there was no increase in the number of fungi in macrophages from vaccinated mice, only the macrophages from BCG-treated mice contained fewer fungi after 48 h of infection with the mycelial phase of H. capsulatum. Fungal growth was not inhibited in any of the macrophage cultures when infected with the yeast phase. The macrophages infected with yeast cells were destroyed after 48 to 72 h in the culture. Only BCG-treated macrophages survived infection with the mycelial phase, whereas macrophages from normal and vaccinated mice were destroyed by the infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berry C. L. The production of disseminated histoplasmosis in the mouse: the eff4ects of changes in reticulo-endothelial function. J Pathol. 1969 Mar;97(3):441–457. doi: 10.1002/path.1710970304. [DOI] [PubMed] [Google Scholar]
  2. Blanden R. V., Lefford M. J., Mackaness G. B. The host response to Calmette-Guérin bacillus infection in mice. J Exp Med. 1969 May 1;129(5):1079–1107. doi: 10.1084/jem.129.5.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boros D. L., Warren K. S. Delayed hypersensitivity-type granuloma formation and dermal reaction induced and elicited by a soluble factor isolated from Schistosoma mansoni eggs. J Exp Med. 1970 Sep 1;132(3):488–507. doi: 10.1084/jem.132.3.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boros D. L., Warren K. S. The bentonite granuloma. Characterization of a model system for infectious and foreign body granulomatous inflammation using soluble mycobacterial, histoplasma and schistosoma antigens. Immunology. 1973 Mar;24(3):511–529. [PMC free article] [PubMed] [Google Scholar]
  5. FURCOLOW M. L. Airborne histoplasmosis. Bacteriol Rev. 1961 Sep;25:301–309. doi: 10.1128/br.25.3.301-309.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goodwin R. A., Jr, Des Prez R. M. State of the art: histoplasmosis. Am Rev Respir Dis. 1978 May;117(5):929–956. doi: 10.1164/arrd.1978.117.5.929. [DOI] [PubMed] [Google Scholar]
  7. HOWARD D. H. INTRACELLULAR GROWTH OF HISTOPLASMA CAPSULATUM. J Bacteriol. 1965 Feb;89:518–523. doi: 10.1128/jb.89.2.518-523.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holt P. G. Alveolar macrophages. I. A simple technique for the preparation of high numbers of viable alveolar macrophages from small laboratory animals. J Immunol Methods. 1979;27(2):189–198. doi: 10.1016/0022-1759(79)90264-3. [DOI] [PubMed] [Google Scholar]
  9. Howard D. H. Further studies on the inhibition of Histoplasma capsulatum within macrophages from immunized animals. Infect Immun. 1973 Oct;8(4):577–581. doi: 10.1128/iai.8.4.577-581.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Howard D. H., Otto V., Gupta R. K. Lymphocyte-mediated cellular immunity in histoplasmosis. Infect Immun. 1971 Nov;4(5):605–610. doi: 10.1128/iai.4.5.605-610.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Leake E. S., Myrvik Q. N. Changes in morphology and in lysozyme content of free alveolar cells after the intravenous injection of killed BCG in oil. J Reticuloendothel Soc. 1968 Feb;5(1):33–53. [PubMed] [Google Scholar]
  12. Montarroso A. M., Myrvik Q. N. Effect of BCG vaccination on the IgG and complement receptors on rabbit alveolar macrophages. J Reticuloendothel Soc. 1978 Aug;24(2):93–99. [PubMed] [Google Scholar]
  13. Sher N. A., Chaparas S. D., Greenberg L. E., Bernard S. Effects of BCG, Corynebacterium parvum, and methanol-extration residue in the reduction of mortality from Staphylococcus aureus and Candida albicans infections in immunosuppressed mice. Infect Immun. 1975 Dec;12(6):1325–1330. doi: 10.1128/iai.12.6.1325-1330.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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