Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Oct;34(1):30–35. doi: 10.1128/iai.34.1.30-35.1981

Nonspecific suppression of primary antibody responses and presence of plastic-adherent suppressor cells in Toxoplasma gondii-infected mice.

Y Suzuki, N Watanabe, A Kobayashi
PMCID: PMC350816  PMID: 6457801

Abstract

The effect of Toxoplasma infection on primary antibody responses to both T-dependent and T-independent antigens was examined in mice. Drastic suppression of primary responses to sheep erythrocytes (SRBC) occurred when mice were immunized 7 days after infection. The suppression was observed in both 2-mercaptoethanol-sensitive and -resistant hemagglutinin responses. Anti-dinitrophenol (DNP) immunoglobulin E and G1 responses to DNP-conjugated keyhole limpet hemocyanin were also suppressed by infection. It was suggested that the suppressive effect is nonspecific for the antigens and immunoglobulin classes produced. Anti-DNP responses to DNP-Ficoll, a T-independent antigen, were suppressed by infection, but the suppressive effect was weaker than that on the responses to SRBC. This suggests that both T and B cells are suppressed by infection. In vitro responses of infected mouse spleen cells to SRBC and DNP-Ficoll confirmed the results of in vivo experiments. In addition, the presence of plastic-adherent suppressor cells was demonstrated in the spleen cells of infected mice, which suppressed the ability of normal mouse spleen cells to mount an SRBC-specific plaque-forming cell response. These plastic-adherent suppressor cells appeared to be a major cause of nonspecific suppression of primary antibody responses in Toxoplasma-infected mice.

Full text

PDF
30

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bloom B. R. Games parasites play: how parasites evade immune surveillance. Nature. 1979 May 3;279(5708):21–26. doi: 10.1038/279021a0. [DOI] [PubMed] [Google Scholar]
  3. Corrêa M., Narayanan P. R., Miller H. C. Suppressive activity of splenic adherent cells from Plasmodium chabaudi-infected mice. J Immunol. 1980 Aug;125(2):749–754. [PubMed] [Google Scholar]
  4. Huldt G., Gard S., Olovson S. G. Effect of Toxoplasma gondii on the thymus. Nature. 1973 Aug 3;244(5414):301–303. doi: 10.1038/244301a0. [DOI] [PubMed] [Google Scholar]
  5. Ishizaka K. Cellular events in the IgE antibody response. Adv Immunol. 1976;23:1–75. doi: 10.1016/s0065-2776(08)60318-1. [DOI] [PubMed] [Google Scholar]
  6. Kirchner H., Holden H. T., Herberman Splenic suppressor macrophages induced in mice by injection of Corynebacterium parvum. J Immunol. 1975 Nov;115(5):1212–1216. [PubMed] [Google Scholar]
  7. Klimpel G. R., Henney C. S. BCG-induced suppressor cells. I. Demonstration of a macrophage-like suppressor cell that inhibits cytotoxic T cell generation in vitro. J Immunol. 1978 Feb;120(2):563–569. [PubMed] [Google Scholar]
  8. Kolb J. P., Arrian S., Zolla-Pazner S. Suppression of the humoral immune response by plasmacytomas: mediation by adherent mononuclear cells. J Immunol. 1977 Feb;118(2):702–709. [PubMed] [Google Scholar]
  9. Mendes R. P., Takehara H. A., Mota I. Trypanosoma cruzi: homocytotrophic antibody response in mice immunized with unrelated antigens. Exp Parasitol. 1979 Dec;48(3):345–351. doi: 10.1016/0014-4894(79)90118-8. [DOI] [PubMed] [Google Scholar]
  10. Mota I., Wong D. Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci. 1969 Aug 15;8(16):813–820. doi: 10.1016/0024-3205(69)90099-x. [DOI] [PubMed] [Google Scholar]
  11. Ovary Z., Caiazza S. S., Kojima S. PCA reactions with mouse antibodies in mice and rats. Int Arch Allergy Appl Immunol. 1975;48(1):16–21. doi: 10.1159/000231289. [DOI] [PubMed] [Google Scholar]
  12. Pierce C. W., Johnson B. M., Gershon H. E., Asofsky R. Immune responses in vitro. 3. Development of primary gamma-M, gamma-G, and gamma-A plaque-forming cell responses in mouse spleen cell cultures stimulated with heterologous erythrocytes. J Exp Med. 1971 Aug 1;134(2):395–416. doi: 10.1084/jem.134.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  14. Ruskin J., McIntosh J., Remington J. S. Studies on the mechanisms of resistance to phylogenetically diverse intracellular organisms. J Immunol. 1969 Aug;103(2):252–259. [PubMed] [Google Scholar]
  15. Strickland G. T., Ahmed A., Sell K. W. Blastogenic response of Toxoplasma-infected mouse spleen cells to T- and B-cell mitogens. Clin Exp Immunol. 1975 Oct;22(1):167–176. [PMC free article] [PubMed] [Google Scholar]
  16. Strickland G. T., Petitt L. E., Voller A. Immunodepression in mice infected with Toxoplasma gondii. Am J Trop Med Hyg. 1973 Jul;22(4):452–455. doi: 10.4269/ajtmh.1973.22.452. [DOI] [PubMed] [Google Scholar]
  17. Strickland G. T., Sayles P. C. Depressed antibody responses to a thymus-dependent antigen in toxoplasmosis. Infect Immun. 1977 Jan;15(1):184–190. doi: 10.1128/iai.15.1.184-190.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Varesio L., Holden H. T. Suppression of lymphokine production. I. Macrophage-mediated inhibition of MIF production. Cell Immunol. 1980 Nov;56(1):16–28. doi: 10.1016/0008-8749(80)90077-5. [DOI] [PubMed] [Google Scholar]
  19. Wellhausen S. R., Mansfield J. M. Characteristics of the splenic suppressor cell--target cell interaction in experimental African trypanosomiasis. Cell Immunol. 1980 Sep 1;54(2):414–424. doi: 10.1016/0008-8749(80)90221-x. [DOI] [PubMed] [Google Scholar]
  20. Wellhausen S. R., Mansfield J. M. Lymphocyte function in experimental African trypanosomiasis. II. Splenic suppressor cell activity. J Immunol. 1979 Mar;122(3):818–824. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES