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. 1981 Oct;34(1):140–143. doi: 10.1128/iai.34.1.140-143.1981

Mitogenicity of influenza hemagglutinin glycoproteins and influenza viruses bearing H2-hemagglutinin.

R B Armstrong, G M Butchko, S C Kiley, M A Phelan, F A Ennis
PMCID: PMC350833  PMID: 7298178

Abstract

The hemagglutinin glycoprotein is responsible for the mitogenic effect of influenza A viruses of the H2N2 subtype. This was indicated by the ability of viruses bearing the H2-hemagglutinin glycoprotein, regardless of its associated neuraminidase, to induce lymphocyte proliferation in normal spleen cell suspensions and by the ability of antisera with specificity for the H2-hemagglutinin to block this response. Moreover, purified hemagglutinin from representative viruses from the H0N1, H1N1, H2N2, H3N2, and influenza B subtypes were also shown to be mitogenic.

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Selected References

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  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyle W. An extension of the 51Cr-release assay for the estimation of mouse cytotoxins. Transplantation. 1968 Sep;6(6):761–764. doi: 10.1097/00007890-196809000-00002. [DOI] [PubMed] [Google Scholar]
  3. Brand C. M., Skehel J. J. Crystalline antigen from the influenza virus envelope. Nat New Biol. 1972 Aug 2;238(83):145–147. doi: 10.1038/newbio238145a0. [DOI] [PubMed] [Google Scholar]
  4. Butchko G. M., Armstrong R. B., Ennis F. A. Specificity studies on the proliferative response of thymus-derived lymphocytes to influenza viruses. J Immunol. 1978 Dec;121(6):2381–2385. [PubMed] [Google Scholar]
  5. Butchko G. M., Armstrong R. B., Martin W. J., Ennis F. A. Influenza A viruses of the H2N2 subtype are lymphocyte mitogens. Nature. 1978 Jan 5;271(5640):66–67. doi: 10.1038/271066a0. [DOI] [PubMed] [Google Scholar]
  6. Compans R. W., Klenk H. D., Caliguiri L. A., Choppin P. W. Influenza virus proteins. I. Analysis of polypeptides of the virion and identification of spike glycoproteins. Virology. 1970 Dec;42(4):880–889. doi: 10.1016/0042-6822(70)90337-5. [DOI] [PubMed] [Google Scholar]
  7. Dowdle W. R., Galphin J. C., Coleman M. T., Schild G. C. A simple double immunodiffusion test for typing influenza viruses. Bull World Health Organ. 1974;51(3):213–215. [PMC free article] [PubMed] [Google Scholar]
  8. Kirchner H., Darai G., Hirt H. M., Keyssner K., Munk K. In vitro mitogenic stimulation of murine spleen cells by herpes simplex virus. J Immunol. 1978 Feb;120(2):641–645. [PubMed] [Google Scholar]
  9. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  10. Phelan M. A., Mayner R. E., Bucher D. J., Ennis F. A. Purification of influenza virus glycoproteins for the preparation and standardization of immunological potency testing reagents. J Biol Stand. 1980;8(3):233–242. doi: 10.1016/s0092-1157(80)80039-4. [DOI] [PubMed] [Google Scholar]
  11. Schild G. C. Evidence for a new type-specific structural antigen of the influenza virus particle. J Gen Virol. 1972 Apr;15(1):99–103. doi: 10.1099/0022-1317-15-1-99. [DOI] [PubMed] [Google Scholar]
  12. Schild G. C., Pereira H. G. Characterization of the ribonucleoprotein and neuraminidase of influenza A viruses by immunodiffusion. J Gen Virol. 1969 Apr;4(3):355–363. doi: 10.1099/0022-1317-4-3-355. [DOI] [PubMed] [Google Scholar]
  13. Sharon N. Lectins. Sci Am. 1977 Jun;236(6):108-16, 118-9. doi: 10.1038/scientificamerican0677-108. [DOI] [PubMed] [Google Scholar]
  14. Woodruff J. J., Woodruff J. F. Influenza A virus interaction with murine lymphocytes. I. The influence of influenza virus A/Japan 305 (H2N2) on the pattern of migration of recirculating lymphocytes. J Immunol. 1976 Sep;117(3):852–858. [PubMed] [Google Scholar]

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