Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Nov;34(2):373–377. doi: 10.1128/iai.34.2.373-377.1981

Effect of proteins on the immunogenicity of enterobacterial common antigen.

H M Kuhn, G Adamus, E Romanowska, H Mayer
PMCID: PMC350876  PMID: 7309231

Abstract

Enterobacterial common antigen isolated by two independent extraction procedures was found to precipitate with a number of basic or hydrophobic proteins. Complexes of enterobacterial common antigen with protamine sulfate, with methylated bovine serum albumin or with a fraction of outer membrane proteins of two different Shigella wild types proved to be highly immunogenic in rabbits upon intravenous immunization, in contrast to the enterobacterial common antigen preparations by themselves. This explains why crude isolates of enterobacterial common antigen usually are good immunogens in contrast to the isolated antigen, which was described to be either not or only very poorly immunogenic.

Full text

PDF
373

Images in this article

374Image
on p.374
376Image
on p.376

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agarwal M. K., Neter E. Effect of selected lipids and surfactants on immunogenicity of several bacterial antigens. J Immunol. 1971 Nov;107(5):1448–1456. [PubMed] [Google Scholar]
  2. Domingue G., Johnson E. Isolation of subcellular fractions containing immunogenic enterobacterial common antigen. Z Immunitatsforsch Exp Klin Immunol. 1974 Nov;148(1):23–38. [PubMed] [Google Scholar]
  3. Geyer R., Galanos C., Westphal O., Golecki J. R. A lipopolysaccharide-binding cell-surface protein from Salmonella minnesota. Isolation, partial characterization and occurrence in different Enterobacteriaceae. Eur J Biochem. 1979 Jul;98(1):27–38. doi: 10.1111/j.1432-1033.1979.tb13156.x. [DOI] [PubMed] [Google Scholar]
  4. Kiss P., Rinno J., Schmidt G., Mayer H. Structural studies on the immunogenic form of the enterobacterial common antigen. Eur J Biochem. 1978 Jul 17;88(1):211–218. doi: 10.1111/j.1432-1033.1978.tb12440.x. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lugowski C., Romanowska E. Enterobacterial common antigen: isolation from Shigella sonnei, purification and immunochemical characterization. Eur J Biochem. 1978 Nov 2;91(1):89–97. doi: 10.1111/j.1432-1033.1978.tb20941.x. [DOI] [PubMed] [Google Scholar]
  7. Marx A., Petcovici M. Immunochemical studies on purified common enterobacterial antigen (KUNIN). Zentralbl Bakteriol Orig A. 1975 Dec;233(4):486–494. [PubMed] [Google Scholar]
  8. Mayer H., Schmidt G. Chemistry and biology of the enterobacterial common antigen (ECA). Curr Top Microbiol Immunol. 1979;85:99–153. doi: 10.1007/978-3-642-67322-1_3. [DOI] [PubMed] [Google Scholar]
  9. Mäkelä P. H., Mayer H. Enterobacterial common antigen. Bacteriol Rev. 1976 Sep;40(3):591–632. doi: 10.1128/br.40.3.591-632.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Männel D., Mayer H. Isolation and chemical characterization of the enterobacterial common antigen. Eur J Biochem. 1978 May 16;86(2):361–370. doi: 10.1111/j.1432-1033.1978.tb12318.x. [DOI] [PubMed] [Google Scholar]
  11. Männel D., Mayer H. Serological and immunological properties of isolated enterobacterial common antigen. Eur J Biochem. 1978 May 16;86(2):371–379. doi: 10.1111/j.1432-1033.1978.tb12319.x. [DOI] [PubMed] [Google Scholar]
  12. NETER E., GORZYNSKI E. A., WESTPHAL O., LUDERITZ O., KLUMPP D. J. The effects of protamine and histone on enterobacterial lipopolysaccharides and hemolysis. Can J Microbiol. 1958 Aug;4(4):371–383. doi: 10.1139/m58-039. [DOI] [PubMed] [Google Scholar]
  13. Olson P. R., Weiblen B. J., O'Leary J. J., Moscowitz A. J., McCullough J. A simple technique for the inactivation of IgM antibodies using dithiothreitol. Vox Sang. 1976;30(2):149–159. doi: 10.1111/j.1423-0410.1976.tb02806.x. [DOI] [PubMed] [Google Scholar]
  14. Rinno J., Golecki J. R., Mayer H. Localization of enterobacterial common antigen:immunogenic and nonimmunogenic enterobacterial common antigen-containing Escherichia coli. J Bacteriol. 1980 Feb;141(2):814–821. doi: 10.1128/jb.141.2.814-821.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SUZUKI T., GORZYNSKI E. A., NETER E. SEPARATION BY ETHANOL OF COMMON AND SOMATIC ANTIGENS OF ENTEROBACTERIACEAE. J Bacteriol. 1964 Nov;88:1240–1243. doi: 10.1128/jb.88.5.1240-1243.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schnaitman C. A. Outer membrane proteins of Escherichia coli. 3. Evidence that the major protein of Escherichia coli O111 outer membrane consists of four distinct polypeptide species. J Bacteriol. 1974 May;118(2):442–453. doi: 10.1128/jb.118.2.442-453.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Whang H. Y., Mayer H., Schmidt G., Neter E. Immunogenicity of the common enterobacterial antigen produced by smooth and rough strains. Infect Immun. 1972 Oct;6(4):533–539. doi: 10.1128/iai.6.4.533-539.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Whang H. Y., Neter E. Antigen-associated immunosuppressant: effect of serum on immune response. Science. 1969 Jan 17;163(3864):290–291. doi: 10.1126/science.163.3864.290. [DOI] [PubMed] [Google Scholar]
  19. Whang H. Y., Neter E. Immunosuppression by endotoxin and its lipoid A component. Proc Soc Exp Biol Med. 1967 Mar;124(3):919–924. doi: 10.3181/00379727-124-31886. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES