Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1981 Nov;34(2):508–512. doi: 10.1128/iai.34.2.508-512.1981

Characterization of Micropolyspora faeni antigens.

V P Kurup, E Y Ting, J N Fink, N J Calvanico
PMCID: PMC350896  PMID: 6171521

Abstract

Culture filtrate antigens of Micropolyspora faeni grown in a synthetic medium in a stirred fermentor were characterized. The culture filtrate antigens were fractionated by preparative isoelectric focusing with a pH gradient of 3.5 to 5.5. The fractions were pooled according to their reaction with rabbit anti-M. faeni sera. A pool containing two major antigens which were resolved by analytical isoelectric focusing and polyacrylamide disc gel electrophoresis was obtained. One antigen was stainable with Coomassie blue and periodic acid-Schiff stain and was determined to have a mass of 51,000 daltons. The other antigen was stainable only with Coomassie blue and was determined to have a mass of 29,000 daltons. When used at 1 mg/ml, this pool reacted with the sera from all patients with farmer's lung disease by immunodiffusion but failed to react with control sera.

Full text

PDF
508

Images in this article

510Image
on p.510
510Image
on p.510
510Image
on p.510
510Image
on p.510
511Image
on p.511

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. Edwards J. H. The double dialysis method of producing farmer's lung antigens. J Lab Clin Med. 1972 Apr;79(4):683–688. [PubMed] [Google Scholar]
  4. Edwards J. H. The isolation of antigens associated with farmer's lung. Clin Exp Immunol. 1972 Jul;11(3):341–355. [PMC free article] [PubMed] [Google Scholar]
  5. Fletcher S. M., Rondle C. J., Murray I. G. The extracellular antigens of Micropolyspora faeni: their significance in farmer's lung disease. J Hyg (Lond) 1970 Sep;68(3):401–409. doi: 10.1017/s0022172400042303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KOBAYASHI M., STAHMANN M. A., RANKIN J., DICKIE H. A. Antigens in moldy hay as the cause of farmer's lung. Proc Soc Exp Biol Med. 1963 Jun;113:472–476. doi: 10.3181/00379727-113-28400. [DOI] [PubMed] [Google Scholar]
  7. Kurup V. P., Fink J. N. A scheme for the identification of thermophilic actinomycetes associated with hypersensitivity pneumonitis. J Clin Microbiol. 1975 Jul;2(1):55–61. doi: 10.1128/jcm.2.1.55-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kurup V. P., Fink J. N. Antigens of Micropolyspora faeni strains. Int Arch Allergy Appl Immunol. 1979;60(2):140–147. doi: 10.1159/000232335. [DOI] [PubMed] [Google Scholar]
  9. Kurup V. P., Fink J. N. Extracellular antigens of Micropolyspora faeni grown in synthetic medium. Infect Immun. 1977 Feb;15(2):608–613. doi: 10.1128/iai.15.2.608-613.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. LaBerge D. E., Stahmann M. A. Antigens from Thermopolyspora polyspora involved in farmer's lung. Proc Soc Exp Biol Med. 1966 Feb;121(2):463–468. doi: 10.3181/00379727-121-30805. [DOI] [PubMed] [Google Scholar]
  11. PEPYS J., JENKINS P. A., FESTENSTEIN G. N., GREGORY P. H., LACEY M. E., SKINNER F. A. FARMER'S LUNG. THERMOPHILIC ACTINOMYCETES AS A SOURCE OF "FARMER'S LUNG HAY" ANTIGEN. Lancet. 1963 Sep 21;2(7308):607–611. doi: 10.1016/s0140-6736(63)90398-2. [DOI] [PubMed] [Google Scholar]
  12. PEPYS J., JENKINS P. A. PRECIPITIN (F.L.H.) TEST IN FARMER'S LUNG. Thorax. 1965 Jan;20:21–35. doi: 10.1136/thx.20.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Radola B. J. Isoelectric focusing in layers of granulated gels. II. Preparative isoelectric focusing. Biochim Biophys Acta. 1975 Mar 28;386(1):181–195. doi: 10.1016/0005-2795(75)90258-5. [DOI] [PubMed] [Google Scholar]
  14. Roberts R. C. Fractionation and chemical characterization studies on Micropolyspora faeni antigens. Ann N Y Acad Sci. 1974;221:199–204. doi: 10.1111/j.1749-6632.1974.tb28217.x. [DOI] [PubMed] [Google Scholar]
  15. Svendsen P. J. Fused rocket immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:69–70. doi: 10.1111/j.1365-3083.1973.tb03781.x. [DOI] [PubMed] [Google Scholar]
  16. Weber K., Pringle J. R., Osborn M. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol. 1972;26:3–27. doi: 10.1016/s0076-6879(72)26003-7. [DOI] [PubMed] [Google Scholar]
  17. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. Glycoprotein staining following electrophoresis on acrylamide gels. Anal Biochem. 1969 Jul;30(1):148–152. doi: 10.1016/0003-2697(69)90383-2. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES