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. 1981 Nov;34(2):526–534. doi: 10.1128/iai.34.2.526-534.1981

Subpopulations of suppressor cells in chickens infected with cells of a transplantable lymphoblastic leukemia.

G A Theis
PMCID: PMC350899  PMID: 6458565

Abstract

Three distinct subpopulations of cells with suppressor activity were separated by Ficoll density gradient centrifugation methods from the spleens of 3- to 4- week-old chickens infected with cultured lymphoblastoid cells (JM-VLC), derived from JM-V leukemia: (i) a subpopulation of nonadherent cells, which separated in the T-cell-rich gradient fraction of leukemic chicken spleen, inhibited proliferative responses to concanavalin A in mixed cultures with normal chicken spleen cells; (ii) phagocytic cells (macrophages), which were the most effective suppressor cells of all subpopulations in the mixed culture assay, were recovered among the cells of greatest density in the spleens of both normal and leukemic chickens; (iii) JM-VLC cells in the buoyant gradient fractions of leukemic chicken spleens also effected suppression. In the later stages of lymphoproliferative disease, the number of spleen cells of buoyant density was increased, apparently as a result of infiltration of the spleens with JM-VLC cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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