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. 1981 Dec;34(3):780–786. doi: 10.1128/iai.34.3.780-786.1981

Induction of C3b-mediated phagocytosis in macrophages by distinct populations of lipopolysaccharide-stimulated lymphocytes.

D M Wrigley, P H Saluk
PMCID: PMC350939  PMID: 6977491

Abstract

Unelicited resident peritoneal macrophages do not significantly ingest erythrocytes coated with C3b. However, these resident macrophages can be induced to ingest via the C3b receptor when cocultured with peritoneal or splenic nonadherent cells obtained from mice previously injected with lipopolysaccharide. In this study, the extent of ingestion induced in resident macrophages was dependent on the number of stimulated nonadherent cells cocultured with the macrophages as well as on the amount of lipopolysaccharide injected in the mice from which the nonadherent cells were obtained. The ability of the stimulated nonadherent cells to convert resident macrophages to a state of C3b receptor-mediated ingestion was not abrogated by the inclusion of polymyxin B in the cocultivation medium. To further characterize these nonadherent cells, different lipopolysaccharide-stimulated cells were obtained by either nylon-wool filtration, depletion of C3b receptor-bearing cells, or depletion of Thy 1.2-positive cells. None of these populations by themselves were capable of inducing resident macrophages to ingest via the C3b receptor, whereas unfractionated cells were. However, coculture of resident macrophages with recombinations of splenic nylon-wool effluent (T cell-enriched) or bound (B cell-enriched) nonadherent cells from lipopolysaccharide-injected mice reconstituted the ability to induce ingestion via the C3b receptor. Taken together, these results suggest that one means by which lipopolysaccharide can induce C3b receptor-mediated ingestion by macrophages is through the cooperative effects of stimulated T and B lymphocytes.

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Selected References

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  1. Alexander P., Evans R. Endotoxin and double stranded RNA render macrophages cytotoxic. Nat New Biol. 1971 Jul 21;232(29):76–78. doi: 10.1038/newbio232076a0. [DOI] [PubMed] [Google Scholar]
  2. Berendt M. J., Mezrow G. F., Saluk P. H. Requirement for a radiosensitive lymphoid cell in the generation of lipopolysaccharide-induced rejection of a murine tumor allograft. Infect Immun. 1978 Sep;21(3):1033–1035. doi: 10.1128/iai.21.3.1033-1035.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berendt M. J., North R. J., Kirstein D. P. The immunological basis of endotoxin-induced tumor regression. Requirement for T-cell-mediated immunity. J Exp Med. 1978 Dec 1;148(6):1550–1559. doi: 10.1084/jem.148.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berendt M. J., Saluk P. H. Tumor inhibition in mice by lipopolysaccharide-induced peritoneal cells and an induced soluble factor. Infect Immun. 1976 Oct;14(4):965–969. doi: 10.1128/iai.14.4.965-969.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bianco C., Griffin F. M., Jr, Silverstein S. C. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. doi: 10.1084/jem.141.6.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doe W. F., Henson P. M. Macrophage stimulation by bacterial lipopolysaccharides. I. Cytolytic effect on tumor target cells. J Exp Med. 1978 Aug 1;148(2):544–556. doi: 10.1084/jem.148.2.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. El-Naggar A. K., Van Epps D. E., Williams R. C., Jr Human-B and T-lymphocyte locomotion in response to casein, C5a, and f-met-leu-phe. Cell Immunol. 1980 Dec;56(2):365–373. doi: 10.1016/0008-8749(80)90112-4. [DOI] [PubMed] [Google Scholar]
  8. Galelli A., Parant M., Chedid L. Role of radiosensitive and radioresistant cells in nonspecific resistance to infection of LPS-treated mice. J Reticuloendothel Soc. 1977 Feb;21(2):109–118. [PubMed] [Google Scholar]
  9. Gordon S., Unkeless J. C., Cohn Z. A. Induction of macrophage plasminogen activator by endotoxin stimulation and phagocytosis: evidence for a two-stage process. J Exp Med. 1974 Oct 1;140(4):995–1010. doi: 10.1084/jem.140.4.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Griffin F. M., Jr, Griffin J. A. Augmentation of macrophage complement receptor function in vitro. II. Characterization of the effects of a unique lymphokine upon the phagocytic capabilities of macrophages. J Immunol. 1980 Aug;125(2):844–849. [PubMed] [Google Scholar]
  11. Griffin J. A., Griffin F. M., Jr Augmentation of macrophage complement receptor function in vitro. I. Characterization of the cellular interactions required for the generation of a T-lymphocyte product that enhances macrophage complement receptor function. J Exp Med. 1979 Sep 19;150(3):653–675. doi: 10.1084/jem.150.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  13. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morland B., Kaplan G. Macrophage activation in vivo and in vitro. Exp Cell Res. 1977 Sep;108(2):279–288. doi: 10.1016/s0014-4827(77)80035-9. [DOI] [PubMed] [Google Scholar]
  15. Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nathan C., Nogueira N., Juangbhanich C., Ellis J., Cohn Z. Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi. J Exp Med. 1979 May 1;149(5):1056–1068. doi: 10.1084/jem.149.5.1056. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Neumann C., Sorg C. Sequential expression of functions during macrophage differentiation in murine bone marrow liquid cultures. Eur J Immunol. 1980 Nov;10(11):834–840. doi: 10.1002/eji.1830101107. [DOI] [PubMed] [Google Scholar]
  18. Nogueira N., Cohn Z. A. Trypanosoma cruzi: in vitro induction of macrophage microbicidal activity. J Exp Med. 1978 Jul 1;148(1):288–300. doi: 10.1084/jem.148.1.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. North R. J., Spitalny G. Inflammatory lymphocyte in cell-mediated antibacterial immunity: factors governing the accumulation of mediator T cells in peritoneal exudates. Infect Immun. 1974 Sep;10(3):489–498. doi: 10.1128/iai.10.3.489-498.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nowakowski M., Edelson P. J., Bianco C. Activation of C3H/HeJ macrophages by endotoxin. J Immunol. 1980 Nov;125(5):2189–2194. [PubMed] [Google Scholar]
  21. Ruco L. P., Meltzer M. S. Macrophage activation for tumor cytotoxicity: development of macrophage cytotoxic activity requires completion of a sequence of short-lived intermediary reactions. J Immunol. 1978 Nov;121(5):2035–2042. [PubMed] [Google Scholar]
  22. Sober A. J., Haynie M., David J. R. Enhanced uptake of 3H-glucosamine by macrophages stimulated by macrophage-activating factor. J Immunol. 1979 May;122(5):1731–1735. [PubMed] [Google Scholar]
  23. Trizio D., Cudkowicz G. Separation of T and B lymphocytes by nylon wool columns: evaluation of efficacy by functional assays in vivo. J Immunol. 1974 Oct;113(4):1093–1097. [PubMed] [Google Scholar]
  24. Vassalli J. D., Reich E. Macrophage plasminogen activator: induction by products of activated lymphoid cells. J Exp Med. 1977 Feb 1;145(2):429–437. doi: 10.1084/jem.145.2.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wahl L. M., Wahl S. M., Mergenhagen S. E., Martin G. R. Collagenase production by lymphokine-activated macrophages. Science. 1975 Jan 24;187(4173):261–263. doi: 10.1126/science.163038. [DOI] [PubMed] [Google Scholar]

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