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. 2012 Nov 13;3(6):e00459-12. doi: 10.1128/mBio.00459-12

FIG 3 .

FIG 3 

Overexpression of RNAIII reduces fitness. (A) Relative competitive fitness in TSB of the WT strain carrying the pTX vector expressing RNAIII or agrA or without an insert. A relative competitive fitness level of <1 is a result of decreased fitness of pTX-related gene expression. Three stars indicate a significant difference (P < 0.001) from the WT strain carrying the pTX vector without an insert. Means with error bars indicating 95% confidence intervals are shown. (B) RNAIII expression levels in TSB of WT cells carrying the pTX vector, pTX::RNAIII, or pTX::agrA and WT cells not carrying the pTX vector quantified at ODs of 0.15 (white), 0.6 (gray), and 1.2 (black). The relative expression level of RNAIII is set to the WT strain’s RNAIII expression levels at an OD of 0.15. Means with standard deviations are shown. (C) Relative competitive fitness in TSB of ΔRNAIII mutant strains carrying the pTX vector expressing RNAIII or agrA or without an insert. Means with error bars indicating 95% confidence intervals are shown. A star indicates a significant difference (P < 0.05) from the ΔRNAIII mutant strain carrying the pTX vector without an insert. dRn, baseline subtracted fluorescent reading normalized to the reference dye.